2020
DOI: 10.3390/ijms21155544
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Dairy-Inspired Coatings for Bone Implants from Whey Protein Isolate-Derived Self-Assembled Fibrils

Abstract: To improve the integration of a biomaterial with surrounding tissue, its surface properties may be modified by adsorption of biomacromolecules, e.g., fibrils. Whey protein isolate (WPI), a dairy industry by-product, supports osteoblastic cell growth. WPI’s main component, β-lactoglobulin, forms fibrils in acidic solutions. In this study, aiming to develop coatings for biomaterials for bone contact, substrates were coated with WPI fibrils obtained at pH 2 or 3.5. Importantly, WPI fibrils coatings withstood auto… Show more

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Cited by 8 publications
(19 citation statements)
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“…Table 1 shows a summary of the research concerning surface modifications of biomaterials using inorganic and composite coatings to improve osteoconductive and osteoinductive properties of the biomaterials for bone regeneration. [70,71], whey protein isolate [72], collagen [73], and BMP-2 [74]-are organic materials which may be used in the coating process of metallic and ceramic biomaterials for bone tissue engineering applications. Organic coatings are not only characterized by high cytocompatibility and biodegradability, but they may also prevent metallic implants and ceramic materials against corrosion and uncontrolled degradability, respectively [75].…”
Section: Inorganic and Composite Coatingsmentioning
confidence: 99%
See 1 more Smart Citation
“…Table 1 shows a summary of the research concerning surface modifications of biomaterials using inorganic and composite coatings to improve osteoconductive and osteoinductive properties of the biomaterials for bone regeneration. [70,71], whey protein isolate [72], collagen [73], and BMP-2 [74]-are organic materials which may be used in the coating process of metallic and ceramic biomaterials for bone tissue engineering applications. Organic coatings are not only characterized by high cytocompatibility and biodegradability, but they may also prevent metallic implants and ceramic materials against corrosion and uncontrolled degradability, respectively [75].…”
Section: Inorganic and Composite Coatingsmentioning
confidence: 99%
“…Osteoconductive and osteoinductive properties of organic coatings have been confirmed in several studies ( Table 2). For instance, Rabe et al [72] coated glass biomaterial with whey protein isolate fibrils to improve its biological properties. They confirmed that coated samples not only enhanced spreading and re-organization of the cytoskeleton of BMDSCs, but also increased tissue non-specific ALP activity.…”
Section: Inorganic and Composite Coatingsmentioning
confidence: 99%
“…For instance, whey proteins (a by-product of cheesemaking) can form amyloid-like protein nanofibrils, at low pH (≤2), low ionic strength, and high temperature (≥80°C), and the resulting structures are observed to be highly stable under various environmental [94]. These fibrils alone can make different forms of materials, such as hydrogels (via the addition of divalent cation salts), microcapsules (via a layer-by-layer self-assembly technique), and coatings (via adsorption) [95][96][97].…”
Section: Protein Nanofibrilsmentioning
confidence: 99%
“…Collagen and fibronectin are well known to have binding sites for cells and have been extremely widely employed as biomaterial [37,38]. However, they are expensive proteins, and therefore, whey protein could constitute an alternative due to its low cost: WPI preparations, such as BiPro from Davisco Inc. and used in previous studies [39], typically cost tens of US dollars per kg, while collagen preparations used to coat biomaterial surfaces, such as those from Sigma Aldrich and BD Biosciences [40,41], would typically cost hundreds of US dollars per g. Due to a high surface/volume ratio, fibrillar structures are interesting candidates to coat materials since this increases the adherence at the surface. Moreover, compared with globular proteins which may change their conformation after adsorption on the material's surface, fibrillar proteins are unlikely to [42,43].…”
Section: Protein Coatingsmentioning
confidence: 99%