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AbstractScope: Enteroendocrine cell lines are routinely assayed in simple buffers at ~20% oxygen to screen foods for bioactives that boost satiety hormone levels. However, in vivo, enteroendocrine cells are exposed to different phases of food digestion and function at low oxygen concentration ranging from 7.5% in the stomach to 0.5% in the colon-rectal junction. Conclusion: This demonstrates the importance of considering physiological oxygen levels and food digestion along GI tract for reliable in vitro analysis of gut hormone secretion.
Methods and results