Damage and protection of UC blood cells during cryopreservation

Number of viable CD34+ cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation

“…Viability of mononuclear cells is determined using the exclusion dye 7-amino actinomycin D and can be easily combined with absolute CD34 + cell counting to enumerate viable CD34 + cells. 13,20 The accuracy of CD34 + cell enumeration and viability testing was extremely high in a recent study of thawed cord blood samples, 21 providing important validation of the single platform methodology.…”

Cryopreservation of Hematopoietic and Non-Hematopoietic Stem Cells – A Review for the Clinician

“…The controlled rate freezing(CRF) procedure still remains the defined standard in many countries (S. M. Watt, Austin, & S. Armitage, 2007). The principal rationale for CRF as choice is the limited cell damage during the freezing process (Donaldson et al, 1996;Douay, 1985;Yang et al, 2001), particularly at the eutectic transition point. At the eutectic transition point the liquid phase transits into a solid phase and fusion heat is released.…”

“…For bone marrow, peripheral blood as well as cord blood stem cells, it has been shown that clonogenic assays, such as the BFU-E and CFU-GM are compromised fairly early during the course of cryopreservation. In contrast, the recovery of nucleated cells (NC) and CD34+ cells remains relatively well preserved (Attarian, Feng, Buckner, MacLeod, & Rowley, 1996;Hal E Broxmeyer et al, 2003;Buchanan et al, 2004;Cilloni et al, 1999;E J Woods et al, 2000;Xiao & Douglas C Dooley, 2003;Yang et al, 2001;X. B. Zhang et al, 2003).…”

“…The most frequently used cell counting assays are the total nucleated cell count and the count of CD34 positive cells (Kurtz, Seetharaman, N. Greco, & Moroff, 2007;Yang et al, 2001). Increasing numbers of both have been shown to correlate positively with the hematopoietic recovery potential in HSC transplantation (Kurtz, Seetharaman, N. Greco, & Moroff, 2007;Yang et al, 2001).…”

Critical role of flow cytometry in evaluating peripheral blood hematopoietic stem cell grafts

“…This method has also been shown to provide accurate CD34 ϩ counts in postcryopreserved cord blood samples (22 (23) have shown that in postthawed samples, a variable number of 7-AAD-/Syto-16 dull apoptotic cells can be detected. In this study, the nonnecrotic (7-AAD-) nonapoptotic (Syto-16 bright) fraction contained all the viable cells as measured by CFU and transwell migration assays (23).…”