Damage and recovery of hair cells in fish canal (but not superficial) neuromasts after gentamicin exposure

Song, Jiakun; Yan, Hong; Popper, Arthur N.

doi:10.1016/0378-5955(95)00170-0

Cited by 100 publications

(95 citation statements)

References 27 publications

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“…In contrast to mammals, many fish species have the ability to regenerate their inner ear and lateral line hair cells after damage (Kaus, 1987;Lombarte et al, 1993;Song et al, 1995;Scholik and Yan, 2001;Faucher et al, 2006;Smith et al, 2006). Given their capacity for regeneration, fish inner ear hair cells are nowadays a suitable model for investigations on hair cell regeneration and hearing function in animals.…”

Section: Introductionmentioning

confidence: 99%

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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a b s t r a c tFish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: (i) systemic (intravenous) and (ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.

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Section: Introductionmentioning

confidence: 99%

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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“…Jones and Corwin (1996) observed mature hair cells arising over one week in the axolotl after laser-induced damage. However, in the adult oscar, a teleost fish, new hair cell stereocilia bundles located in canal neuromasts were observed as early as two days following prolonged aminoglycoside exposure (Song et al 1995). In addition, behavioral recovery in adult Cyprinodontidae fish is reported to occur one to three days after aminoglycoside treatment (Kaus 1987).…”

Section: Lateral Line Hair Cell Regenerationmentioning

confidence: 99%

“…A final possibility is that there may be hair cell heterogeneity within a neuromast at this point in lateral line development. Based on differential ototoxic sensitivity, type I and type II hair cells have been described in the fish inner ear as well as in canal and superficial neuromasts, respectively, of the adult lateral line (Yan et al 1991;Song et al 1995). Differentiating among these possibilities will require further research.…”

Section: Aminoglycoside-induced Hair Cell Deathmentioning

confidence: 99%

“…Scores as a function of neomycin concentration yielded significant differences at each successive dosage (p < 0.001) except for the comparisons between 250 and 500 lM using phalloidin or acetylated tubulin labeling, which revealed equivalent effects. (Jorgensen 1991;Song et al 1995;Jones and Corwin 1996). We examined neuromast hair cells at 12 and 24 h after neomycin exposure using DASPEI to further understand the changes in hair cell populations in p4 and p5 zebrafish larvae after aminoglycoside treatments.…”

Section: Neomycin Toxicity Of Lateral Line Hair Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Neomycin-Induced Hair Cell Death and Rapid Regeneration in the Lateral Line of Zebrafish ( Danio rerio )

Harris

Cheng

Cunningham

et al. 2003

JARO - Journal of the Association for Research in Otolaryngolog

438

573

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Mechanoreceptive hair cells are extremely sensitive to aminoglycoside antibiotics, including neomycin. Hair cell survival was assessed in larval wild-type zebrafish lateral line neuromasts 4 h after initial exposure to a range of neomycin concentrations for 1 h. Each of the lateral line neuromasts was scored in live fish for the presence or absence of hair cells using the fluorescent vital dye DASPEI to selectively label hair cells. All neuromasts were devoid of DASPEI-labeled hair cells 4 h after 500 lM neomycin exposure. Vital DASPEI staining was proportional to the number of hair cells per neuromast identified in fixed larvae using immunocytochemistry for acetylated tubulin and phalloidin labeling. The time course of hair cell regeneration in the lateral line neuromasts was also analyzed following neomycin-induced damage. Regenerated hair cells were first observed using live DASPEI staining 12 and 24 h following neomycin treatment. The potential role of proliferation in regenerating hair cells was analyzed. A 1 h pulse-fix protocol using bromodeoxyuridine (BrdU) incorporation was used to identify S-phase cells in neuromasts. BrdU incorporation in neomycin-damaged neuromasts did not differ from control neuromasts 4 h after drug exposure but was dramatically upregulated after 12 h. The proliferative cells identified during a 1 h period at 12 h after neomycin treatment were able to give rise to new hair cells by 24-48 h after drug treatment. The results presented here provide a standardized preparation for studying and identifying genes that influence vertebrate hair cell death, survival, and regeneration following ototoxic insults.

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“…temporary blinding (Pitcher et al, 1976;Partridge and Pitcher, 1980), and chemical (e.g. Karlsen and Sand, 1987;Kaus, 1987;Song et al, 1995) or physical (e.g. Montgomery et al, 1997; ablation of the lateral line submodalities.…”

Section: What Is the Optimal Design For Fish Passage Structures?mentioning

confidence: 99%

Experimentation at the interface of fluvial geomorphology, stream ecology and hydraulic engineering and the development of an effective, interdisciplinary river science

Rice

Lancaster

Kemp

2009

Earth Surf Processes Landf

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One "2020 vision" for fluvial geomorphology is that it sits alongside stream ecology and hydraulic engineering as a key element of an integrated, interdisciplinary river science. A challenge to this vision is that scientists from these three communities may approach problems from different perspectives with different questions and have different methodological outlooks. Refining interdisciplinary methodology is important in this context, but raises a number of issues for geomorphologists, ecologists and engineers alike. In particular, we believe that it is important that there is greater dialogue about the nature of mutually-valued questions and the adoption of mutually-acceptable methods. As a contribution to this dialogue we examine the benefits and challenges of using physical experimentation in flume laboratories to ask interdisciplinary questions. Working in this arena presents the same challenges that experimental geomorphologists and engineers are familiar with (scaling up results, technical difficulties, realism) and some new ones including recognising the importance of biological processes, identifying hydraulically meaningful biological groups, accommodating the singular behaviour of individuals, understanding biological as well as physical stimuli, and the husbandry and welfare of live organisms. These issues are illustrated using two examples from flume experiments designed (1) to understand how the movement behaviours of aquatic insects through the near-bed flow field of gravelly river beds may allow them to survive flood events, and (2) how an understanding of the way in which fish behaviours and swimming capability are affected by flow conditions around artificial structures can lead to the design of effective fish passages. In each case, an interdisciplinary approach has been of substantial mutual benefit and led to greater insights than discipline-specific work would have produced. Looking forward to 2020, several key challenges for experimentalists working on the interface of fluvial geomorphology, stream ecology and hydraulic engineering are identified.3

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Damage and recovery of hair cells in fish canal (but not superficial) neuromasts after gentamicin exposure

Cited by 100 publications

References 27 publications

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Neomycin-Induced Hair Cell Death and Rapid Regeneration in the Lateral Line of Zebrafish ( Danio rerio )

Experimentation at the interface of fluvial geomorphology, stream ecology and hydraulic engineering and the development of an effective, interdisciplinary river science

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