Damage and survivorship of fall armyworm (Lepidoptera: Noctuidae) on transgenic field corn expressing Bacillus thuringiensis Cry proteins

Hardke, J. T.; Leonard, B. R.; Huang, Fangneng; Jackson, R. E.

doi:10.1016/j.cropro.2010.10.005

Cited by 76 publications

(53 citation statements)

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“…Insecticide spraying caused a significant spraying the damage scores of hybrids with protein Cry1Ab were similar to those with Cry1F technology, this type of management increases production costs (Table 5). The fact that the hybrid expressing protein Cry1F is more effective in reducing S. frugiperda damage than hybrids expressing protein Cry1Ab was also reported by Siebert et al (2008aSiebert et al ( , 2008b and Hardke et al (2011).…”

Section: Resultsmentioning

confidence: 48%

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Eficácia de milho transgênico tratado com inseticida no controle da lagarta-do-cartucho no milho safrinha no estado de São Paulo, Brasil

et al. 2017

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Several genetically modified maize events expressing insecticidal proteins from Bacillus thuringiensis (Bt) have been commercially available in Brazil, intended to control of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). The objective of this study was to evaluate the efficiency of transgenic (Bt) and conventional (non-Bt) hybrids in the control of fall armyworm, treated or not with insecticides throughout three late growing seasons. To this end, trials were conducted at three locations in the state of São Paulo, in a randomized complete block design, in factorial arrangements with 5x4, 5x4 and 8x4 factors, respectively. The first factor consisted of the number of commercial hybrids of different companies. The second factor was represented by the different control managements of insect (non-Bt hybrid with and without insecticide; Bt hybrid with and without insecticide). The damage caused by fall armyworm was evaluated on a visual 1-9 scale, from score 0 (no damage) to 9 (totally destroyed whorl), and grain yield (kg ha -1 ). The data were analyzed by analysis of variance and the means compared by the Tukey test at 5% probability for each parameter evaluated at each location. For the Bt hybrids, the damage scores attributed to fall armyworm were lower and the proteins Cry 1F, Cry 1A105 and VIP3Aa20 were the most efficient in reducing leaf damage. Insecticide applications proved efficient in reducing leaf damage caused by the pest. Application of insecticides to Bt hybrids generally reduced leaf damage, especially for Cry1Ab-producing hybrids, which were the least effective in reducing pest damage without insecticides.Index terms: Zea mays; Spodoptera frugiperda; pest management. RESUMODiversas tecnologias de milho geneticamente modificado (Bt) foram liberadas comercialmente desde 2007 visando principalmente o controle da lagarta-do-cartucho, Spodoptera frugiperda (Lepidoptera: Noctuidae). O objetivo deste trabalho foi avaliar a eficiência de híbridos transgênicos (Bt) e convencionais (não Bt) no controle lagarta-do-cartucho, submetidas ou não a inseticidas. Para isso, foram conduzidos ensaios em três localidades do estado de São Paulo nas safrinhas de 2009, 2010 e 2011, com delineamento experimental de blocos casualizados, em esquema fatorial 5x4, 5x4 e 8x4, respectivamente. O primeiro fator correspondeu ao número de híbridos comerciais de diferentes empresas. O segundo fator foi constituído pela utilização de diferentes manejos de controle do inseto. Para a avaliação dos danos ocasionados pela lagarta-do-cartucho, foram realizadas amostragens ao acaso de 20 plantas por parcela, aplicando-se uma escala de notas visuais, atribuindo notas que variam de 0 (sem dano) a 9 (cartucho totalmente destruído) e obtido a produtividade de grãos. Os dados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade, para cada parâmetro avaliado em cada localidade. Os híbridos transgênicos proporcionaram redução nas notas de danos atribuídas a lagarta-do-cartu...

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Section: Resultsmentioning

confidence: 48%

“…In the Northwest, the experiment was installed in Votuporanga, in the Central North in the city of Pindorama and in Mid Paranapanema, in the municipality of Maracaí in 2009and 2011and in Paraguaçu Paulista in 2010 In the experiments carried out in Pindorama the crops were irrigated.…”

Section: Methodsmentioning

confidence: 99%

Eficácia de milho transgênico tratado com inseticida no controle da lagarta-do-cartucho no milho safrinha no estado de São Paulo, Brasil

et al. 2017

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“…Keller et al (1996) demonstraram que o suco intestinal dos últimos ínstares de S. littoralis tem alta atividade proteolílica, capaz de degradar completamente a proteína Cry 1C. Hardke et al (2011) também observaram alta sobrevivência larval de S. frugiperda -cerca de 83% -, ao se alimentar do híbrido DKC 67-03 YGCB.…”

Section: Resultsunclassified

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Mendes

Boregas

Lopes

et al. 2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar parâmetros biológicos da lagarta-do-cartucho do milho (Spodoptera frugiperda), alimentada com híbridos de milho Bt, que expressam a toxina Cry 1A(b), e com seus respectivos isogênicos não Bt. Os experimentos foram realizados no laboratório da Embrapa Milho e Sorgo, em Sete Lagoas, MG. Os parâmetros avaliados foram: sobrevivência de larvas após 48 horas, sobrevivência da fase larval e pré-imaginal, biomassa de larvas aos 14 dias de idade, biomassa de pupas, período de desenvolvimento larval, e não preferência alimentar de larvas do primeiro ínstar. Larvas de S. frugiperda apresentam menor sobrevivência nas primeiras 48 horas de alimentação e durante toda a fase larval, na maioria dos híbridos de milho Bt, em comparação ao milho não Bt. A biomassa de larvas e pupas foi sempre menor no milho Bt, e o período larval e o pré-imaginal, maior. Houve interação entre a toxina Cry 1A(b) e a base genética dos híbridos transgênicos, quanto à sobrevivência e à biomassa larval. Larvas recém-eclodidas de S. frugiperda apresentam preferência pela alimentação em milho não Bt.Termos para indexação: Spodoptera frugiperda, manejo de resistência de insetos, manejo integrado de pragas, resistência de plantas, transgenia. Fall armyworm responses to genetically modified maize expressing the toxin Cry 1A(b)Abstract -The objective of this work was to evaluate biological parameters of maize fall armyworm (Spodoptera frugiperda) fed with Bt maize hybrids expressing the Cry 1A(b) toxin, and with its isogenic non-Bt hybrids. The experiments were carried out in the laboratory of Embrapa Milho e Sorgo, Sete Lagoas, MG, Brazil. The parameters evaluated were: survival of larvae after 48 hours, survival of larval and preimaginal phases, larvae biomass at 14 days of age, pupae biomass, larval development period, and feeding non-preference of first instar larvae. Larvae of S. frugiperda show less resistance in the first 48 hours of feeding and during the entire larval phase in most Bt maize hybrids, in comparison to non-Bt maize. The biomass of larvae and pupae developed in Bt maize was always smaller, and the larval and preimaginal period were longer. There was interaction between the Cry 1A(b) toxin and the genetic base of the transgenic hybrids regarding survival and larval biomass. Newly hatched S. frugiperda larvae show preference for feeding on non-Bt maize.

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“…Smith) (Lepidoptera: Noctuidae), is an insect native to the Americas, occurring from the South and Central Americas to North America (Hardke et al, 2011). This species has a polyphagous feeding habit, and its larval diet includes a broad variety of host plants such as maize, cotton, and rice (Yu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Methodology Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda

Pavinato¹,

Martinelli²,

Lima³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We developed six microsatellite markers for the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The SSR loci were isolated with enriched genomic library protocol by using native individuals as a genome source for markers. These loci were characterized in 48 individuals and they were tested for the ability to identify candidate migrants exchanged among the samples. The number of alleles per locus ranged from 5 to 18 (10.8 on average). The observed polymorphism information content ranged from 0.172 to 0.891. Beside the lower efficiency to obtain SSR loci, the six microsatellites were polymorphic and sufficiently discriminant for the genetic studies of S. frugiperda; it allowed us to identify migrants with both NJ clustering and the Bayesian methods. These markers will be useful for molecular SSRs for genetic studies of fall armyworm ecology studies of this highly polyphagous species in order to understand the processes that determine genetic differentiation in the complex agro-ecosystems that it infests and improve local integrated pest management practices.

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Damage and survivorship of fall armyworm (Lepidoptera: Noctuidae) on transgenic field corn expressing Bacillus thuringiensis Cry proteins

Cited by 76 publications

References 24 publications

Eficácia de milho transgênico tratado com inseticida no controle da lagarta-do-cartucho no milho safrinha no estado de São Paulo, Brasil

Eficácia de milho transgênico tratado com inseticida no controle da lagarta-do-cartucho no milho safrinha no estado de São Paulo, Brasil

Respostas da lagarta‑do‑cartucho a milho geneticamente modificado expressando a toxina Cry 1A(b)

Methodology Microsatellite markers for genetic studies of the fall armyworm, Spodoptera frugiperda

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