Damage of Erythrocytes by Activated Oxygen Generated in Hypoxic Rat Liver

Abstract

The implication of activated oxygen in the interaction between hypoxic rat liver and circulating erythrocytes was investigated. Reduced species of oxygen generated in hypoxic liver owing to accelerated purine nucleotide degradation via xanthine oxidase initiate alterations of plasma membrane and glutathione system of erythrocytes. Osmotic fragility, hemolysis rate and erythrocytic GSSG:GSH ratio may be considered as appropriate indicators of oxidative load in liver and other tissues. Addition of erythrocytes to the perfusion medium attenuates the GSSG efflux of hypoxic liver from 2.7 +/- 0.5 nmol x g w.w.-1 x min-1 to 1.4 +/- 0.2 nmol x g w.w.-1 x min-1 Thus, circulating erythrocytes protect the liver against oxidative attack.

Blood glutathione redox status and global methylation of peripheral blood mononuclear cell DNA in Bangladeshi adults

“…40 A study in rats showed that an increase in ROS in liver initiated changes in the erythrocytic GSH/GSSG ratio; the authors concluded that the erythrocytic GSH/GSSG ratio reflects oxidative stress in liver and other tissues. 41 Additionally, an intact transsulfuration pathway has been identified in blood cells, including macrophages 42 and T cells, 43 and transsulfuration pathway flux is upregulated in naïve and activated T cells after exposure to peroxide. 43 Even concentrations, folic acid as pteroylglutamic acid was used for calibration, and its 125 I-labeled analog was used as the tracer; for vitamin B 12 , cyanocobalamin was used for calibration, and its 57 Co-labeled analog was used as the tracer.…”

Nonenzymatic antioxidants of blood in multiple sclerosis

“…In addition to protecting the host cell, erythrocyte glutathione (GSH) can also efficiently defend other tissues (e.g., lung, liver) [41,47]. The protective mechanism by GSH results in an increased formation and subsequent translocation into plasma of oxidized glutathione (GSSG).…”

Oxidative stress induced by bloodless limb surgery on humans

“…In addition to protecting themselves, eythrocytes can efficiently defend lung, liver and the immature tissues of premature infants from oxidant attack [10][11][12]. Tracheal insufflation of a small number of intact erythrocytes prevents oxidative lung injury, glutathione having been the beneficial erythrocyte constituent [10].…”