Damage prevention effect of milk-derived peptides on UVB irradiated human foreskin fibroblasts and regulation of photoaging related indicators

Xie, Yujia; Wang, Juan; Li, Zheng; Peng, Xiaojie; Li, Mingyi; Luan, Yuanyuan; Xiao, Shanshan; Zhang, Shaohui

doi:10.1016/j.foodres.2022.111798

Cited by 10 publications

(4 citation statements)

References 53 publications

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“…The MMP-1 and MMP-9 secretions are shown in Figure 6b,c. The level of MMP-1 and MMP-9 in MC groups strikingly improved 3.3 times and 5.5 times compared to the NC group, which is consistent with other reports [38], indicating the degradation of the dermal extracellular matrix, particularly types I and III. This result is in accordance with the pathological analysis, which hints at an impediment to collagen synthesis.…”

Section: Biomarkers Related To Collagen Stability and Degradationsupporting

confidence: 91%

The Protective Effects of Silkworm (Bombyx mori) Pupae Peptides on UV-Induced Skin Photoaging in Mice

Lin,

Fan,

et al. 2024

Foods

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Silkworm (Bombyx mori) pupae are popular edible insects with high nutritional and therapeutic value. Currently, there is growing interest in the comprehensive application of silkworm pupae. In this study, peptides that exhibited anti-photoaging activity were obtained from silkworm pupae protein, aiming to investigate the protective effects and potential mechanisms of silkworm pupae peptides (SPPs) on skin photoaging. The results showed that SPPs were composed of 900 short peptides and could effectively alleviate skin photoaging progression. They significantly eliminated excessive production of ROS and MDA; meanwhile, they also renovated the antioxidant enzyme activities. The biomarkers related to collagen synthesis and degradation, including hydroxyproline, interstitial collagenase, and gelatinase, demonstrated that SPPs could suppress collagen degradation. Histopathological results showed that SPPs could reduce the inflammatory infiltrate and the thickness of the dermis and epidermis, as well as increase the collagen bundles and muscle fibers. The histopathological and biochemical results confirmed that SPPs could alleviate photoaging by inhibiting abnormal skin changes, reducing oxidative stress, and immune suppression. Overall, these data prove the protective effects of SPPs against the photoaging process, suggesting their potential as an active ingredient in skin photoaging prevention and therapy.

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Section: Biomarkers Related To Collagen Stability and Degradationsupporting

confidence: 91%

The Protective Effects of Silkworm (Bombyx mori) Pupae Peptides on UV-Induced Skin Photoaging in Mice

Lin,

Fan,

et al. 2024

Foods

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“…Distillers' grains underwent lyophilization using the Christ Alpha 1-2 LD plus lyophilizer and then were finely made into a powder using the Lingdan LD-T300 grinder. The isolation of protein from the distillers' grains followed the way outlined by Wang et al (2019) (minor modifications) [15,16]. The concrete method of separating proteins is displayed in the Supplementary Materials.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The closer the probability of a peptide being biologically active predicted by Peptide Ranker to 1, the more confident Peptide Ranker is that the peptide is biologically active. Peptides with a score above or equal to 0.6 were more likely to be biologically active [16,17]. Based on the scoring results, ten peptides with scores greater than 0.6 were selected, which were RFDR, PDVGHPM, YPLPR, AFEPLR, FEPLR, WLDY, FEPL, FDGVLRGP, NDPF, and WNLN.…”

Section: Screening Of Main Bioactive Peptides In Distillers' Grains P...mentioning

confidence: 99%

Identification of Novel Peptides in Distillers’ Grains as Antioxidants, α-Glucosidase Inhibitors, and Insulin Sensitizers: In Silico and In Vitro Evaluation

Ding,

Zheng,

Zhao

et al. 2024

Nutrients

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Distillers’ grains are rich in protein and constitute a high-quality source of various bioactive peptides. The purpose of this study is to identify novel bioactive peptides with α-glucosidase inhibitory, antioxidant, and insulin resistance-ameliorating effects from distiller’s grains protein hydrolysate. Three novel peptides (YPLPR, AFEPLR, and NDPF) showed good potential bioactivities, and the YPLPR peptide had the strongest bioactivities, whose IC50 values towards α-glucosidase inhibition, radical scavenging rates of 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were about 5.31 mmol/L, 6.05 mmol/L, and 7.94 mmol/L, respectively. The glucose consumption of HepG2 cells treated with YPLPR increased significantly under insulin resistance condition. Moreover, the YPLPR peptide also had a good scavenging effect on intracellular reactive oxygen species (ROS) induced by H2O2 (the relative contents: 102.35% vs. 100%). Molecular docking results showed that these peptides could stably combine with α-glucosidase, ABTS, and DPPH free radicals, as well as related targets of the insulin signaling pathway through hydrogen bonding and van der Waals forces. This research presents a potentially valuable natural resource for reducing oxidative stress damage and regulating blood glucose in diabetes, thereby increasing the usage of distillers’ grains peptides and boosting their economic worth.

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“…Increasing evidence suggested that dietary strategies that inhibiting MMP activity and ROS production have the potential to alleviate skin photoaging (Cao et al, 2020; Solway et al, 2020; Yao et al, 2016). Studies have showed that ingestion of collagen, elastin or their hydrolysates has the potential to attenuate UV‐induced photoaging in the skin (Wang et al, 2021; Xie et al, 2022). Collagen hydrolysates (e.g., Decapterus (Jiang et al, 2014), Elastin of tuna cardiac arterial bulbs (Kong et al, 2023), tilapia (Sun et al, 2013), cod skin gelatin (Lu et al, 2017), and abalone hydrolysate (Nguyen et al, 2013)) supplementations have been found to show beneficial effects on downregulating MMP‐1 activity and reducing ROS level, thereby attenuating UV‐induced collagen degradation and cell anoikis.…”

Section: Introductionmentioning

confidence: 99%

GLGPGVG derived from bovine elastin repairs UV‐induced photoaging in human foreskin fibroblasts and its potential bioavailability

Sun,

Liu,

Liu

et al. 2024

eFood

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Studies have indicated the potential of oral elastin hydrolysates in repairing photoaging skin. To reveal the underlying mechanism, this study examined how two elastin‐derived peptides (Gly‐Leu‐Gly‐Pro‐Gly‐Val‐Gly [GLGPGVG] and Pro‐Tyr [PY]) repair ultraviolet (UV)‐induced damage in human foreskin fibroblasts (HFFs) and explored their digestive stability using UPLC‐MS/MS. Results showed that UV‐damaged HFFs treated with GLGPGVG showed higher superoxide dismutases (SOD) activity and cell viability compared with PY treatment. Furthermore, GLGPGVG even reversed UV‐induced increase in reactive oxygen species, MMP‐12 (elastase), elastin mRNA and intercellular Ca2+ levels, and decrease in elastin content. Intriguingly, in vitro digestion products from GLGPGVG retained approximately 60% of elastase inhibitory activity. Furthermore, a portion of GLGPGVG was observed to pass through the Caco‐2 monolayer intact. These findings revealed that elastin‐derived peptide GLGPGVG holds promise for passing through the gastrointestinal tract and exerting protective effects against photoaging through increasing SOD activity and inhibiting MMP‐12 expression.

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Damage prevention effect of milk-derived peptides on UVB irradiated human foreskin fibroblasts and regulation of photoaging related indicators

Cited by 10 publications

References 53 publications

The Protective Effects of Silkworm (Bombyx mori) Pupae Peptides on UV-Induced Skin Photoaging in Mice

The Protective Effects of Silkworm (Bombyx mori) Pupae Peptides on UV-Induced Skin Photoaging in Mice

Identification of Novel Peptides in Distillers’ Grains as Antioxidants, α-Glucosidase Inhibitors, and Insulin Sensitizers: In Silico and In Vitro Evaluation

GLGPGVG derived from bovine elastin repairs UV‐induced photoaging in human foreskin fibroblasts and its potential bioavailability

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