Damaging effect of cumulus denudation on rabbit oocytes

Lu, Jinhua; Wang, Junzuo; Wang, Huili; Sui, Hong-Shu; Liu, Yong; Li, Jingjing; Liang, Bo; Tan, Jing-He

doi:10.1016/j.fertnstert.2009.05.066

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…Cumulus cells seem to act as a barrier, which protects the oocytes from in vitro-induced lipotoxic effects, such as reactive oxygen species formation, caspase 3 activation, and mitochondria deterioration [27]. Denuding cumulus cells has been known to severely damage oocytes, leading to their apoptosis or degeneration in rabbits [28]. On the other hand, in the present study, any differences in the state of apoptosis of oocytes were not observed when the CCs were removed 20 hours and 44 hours after the start of the culture.…”

Section: Discussionmentioning

confidence: 99%

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes

et al. 2016

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Melatonin improves the maturation and developmental ability of bovine oocytes by up-regulating GJA4 to enhance gap junction intercellular communication

Tang

Xu²,

Huang³

et al. 2021

Reprod. Fertil. Dev.

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Melatonin (MT) increases oocyte maturation by reducing reactive oxygen species level and enhancing oocyte antioxidant capacity. However, the mechanisms via which MT works are still poorly understood. In the present study, the effects of MT on the maturation rate and development ability of bovine oocytes were investigated. Then, the transcriptome of oocytes treated by MT was sequenced. Finally, the expression of gap junction protein alpha 4 (GJA4) protein and cAMP level were detected in bovine oocytes, and isoprenaline (enhancer of gap junctional intercellular communication (GJIC)) and heptanol (inhibitor of GJIC) were used to investigate the effect of MT on GJIC activity in bovine oocytes. Our results showed that MT significantly improved the maturation, developmental ability and mRNA expression of GJA4 of bovine oocytes. Meanwhile, MT significantly increased GJA4 protein level and cAMP level in bovine oocytes. In contrast to heptanol, both isoproterenol and MT significantly increased GJIC activity, nuclear maturation and the development ability of bovine oocytes. However, MT significantly restored the nuclear maturation and developmental ability of oocytes treated by heptanol. In conclusion, our results showed that MT improves the maturation and developmental ability of bovine oocytes by enhancing GJIC activity via up-regulating GJA4 protein expression in IVM progress.

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Effect of cumulus cells and duration of culture on nuclear maturation of oocytes of pubertal and pre-pubertal individuals from Sus scrofa domesticus

Lopukhov

2024

jour

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Objective: to evaluate the nuclear maturation ability of pig oocytes in relation to the morphology of the surrounding cumulus cells, the duration of in vitro maturation (IVM) and the physiological status of cumulus-oocyte complexes (COCs) donor animals.Materials and methods. СOCs collected from cycling (pubertal) and non-cycling (prepubertal) pigs ovaries were categorized according to the number of cumulus cell (CC) layers: COC I – more then 4, COC II – 3-4, COC III – 1-2 and СOC with granulosa cells (GCOC). Oocytes within COCs were matured during the first 22 h of culture in the TC-199 medium with 25 mM HEPES, 3.05 mM D-glucose, 0.91 mM sodium pyruvate, 0.57 mM cysteine, 0.4% BSA, 10% follicular fluid, 0.5 μg/mL FSH, 0.5 μg/mL LH and 50 μg/mL gentamicin sulfate and thereafter in medium of the same composition but without hormones. After 42, 44, 46, 48 hours of culture the maturation of oocytes of all categories and age groups was assessed by the sign of their release of the first polar body. Results. It was shown that at least 42 hours of in vitro culture of pubertal pig oocytes are required to ensure a high level of nuclear maturation. The duration of the in vitro maturation was found to be 44-48 hours for pre-pubertal pigs. Oocytes surrounded by 1-2 layers of CC, when cultured for less than 48 hours, are inferior in nuclear maturation to oocytes with a more multilayered cumulus. The presence of GC (granulosa cells) around COC retards retards the progression of nuclear maturation when cultured for less than 42 hours in prepubertal and 44 hours in pubertal gilts. Oocytes from cycling pigs reach peak maturation earlier than those from non-cycling gilts and can be used in assisted reproductive technologies (ART). Conclusions. It was concluded that both morphology of the surrounding cumulus cells and physiologic status of COC donor animals should be considered when choosing the duration of pig oocytes culture for the purpose of their in vitro maturation.

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Damaging effect of cumulus denudation on rabbit oocytes

Cited by 3 publications

References 46 publications

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes

Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes

Melatonin improves the maturation and developmental ability of bovine oocytes by up-regulating GJA4 to enhance gap junction intercellular communication

Effect of cumulus cells and duration of culture on nuclear maturation of oocytes of pubertal and pre-pubertal individuals from Sus scrofa domesticus

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