Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro

Bo, Huaben; He, Junhua; Wang, Xiao; Du, Ruilan; Bei, Haikang; Wang, Jinquan; Wu, Fenglin; Zhang, Wenfeng; Chen, Qizhu

doi:10.1016/j.jep.2018.11.018

Cited by 17 publications

(4 citation statements)

References 32 publications

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“…The isomer-specific effect was also found in the study of Lee et al [40], in which trans-10,cis-12-CLA suppressed cell proliferation and induced apoptosis in human colorectal cancer, whereas cis-9,trans-11-CLA showed no such effect. The qPCR and transcriptome results further indicated that the inhibitory effects were based on the mechanisms of downregulating the mRNA expression of CDK17, WNT10A and MMP13, as well as the molecular function of fibroblast growth factor-activated receptor activity; pathways of Hippo, PI3K-Akt, TGF-β, Rap1 signaling and focal adhesion in RECs, which are positively involved in cell proliferation and migration [41][42][43][44][45][46][47][48][49]. Accumulating evidence suggests that inflammatory mediators, especially cytokines, such as TNF-α, IL-1β and IL-6, play important roles in regulating the initiation and progression of cell proliferation, survival, migration and apoptosis via their effects on epithelial signaling pathways, such as signal transducer and activator of transcription 3 (STAT3), c-Jun NH2-terminal kinase (JNK), mitogen-activated protein kinase (MAPK) and NF-κB [25,50].…”

Section: Discussionmentioning

confidence: 96%

Isomer-Specific Effects of cis-9,trans-11- and trans-10,cis-12-CLA on Immune Regulation in Ruminal Epithelial Cells

Yang

Zhu

et al. 2021

Animals

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In this study, we used transcriptomics and qPCR to investigate the potential immunoprotective effects of different conjugated linoleic acid (CLA) isomers, the natural rumen microbial metabolites, on lipopolysaccharide (LPS)-induced inflammation of ruminal epithelial cells (RECs) in vitro. The results showed that 100 μM trans-10,cis-12-CLA exerted higher anti-inflammatory effects than cis-9,trans-11-CLA by significantly downregulating the expression of genes related to inflammation, cell proliferation and migration in RECs upon LPS stimulation. Transcriptomic analyses further indicated that pretreatment with trans-10,cis-12-CLA, but not cis-9,trans-11-CLA, significantly suppressed the biological signals of GO terms’ response to LPS, the regulation of signal transduction and cytokine production and KEGG pathways NF-κB, chemokine, NOD-like receptor, Hippo, PI3K-Akt, TGF-β and Rap1 signaling in RECs upon LPS stimulation. Furthermore, pretreatment with trans-10,cis-12-CLA significantly reduced the expression of lipogenic genes and the biosynthesis of the unsaturated fatty acid pathway in RECs compared with the LPS group, however, cis-9,trans-11-CLA exhibited the opposite results. These results suggest the distinct isomer differences of CLA in the regulation of inflammatory responses and adipocytokine signaling in RECs and will provide important references for determining their target use in the future.

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Section: Discussionmentioning

confidence: 96%

Isomer-Specific Effects of cis-9,trans-11- and trans-10,cis-12-CLA on Immune Regulation in Ruminal Epithelial Cells

Yang

Zhu

et al. 2021

Animals

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“…We identified 4 pathways from upregulated DEPs and 14 pathways from downregulated DEPs. The down-regulated genes encoding epithelial intercellular connexin in the tight junction pathway and focal adhesion pathway indicate that there is interference in the permeability between epithelial barriers, which may be closely related to the regulation of intercellular barriers in septic lung injury (56)(57)(58).…”

Section: Discussionmentioning

confidence: 99%

Proteomic and phosphorylated proteomic landscape of injured lung in juvenile septic rats with therapeutic application of umbilical cord mesenchymal stem cells

Wang

Luo²,

Li³

et al. 2022

Front. Immunol.

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Acute lung injury (ALI) is the most common complication of sepsis. Intravenous injection of HUMSCs can regulate the level of circulating endothelial cytokines and alleviate lung injury in juvenile septic rats. In this study, we performed proteomic and phosphorylated proteomic analysis of lung tissue of juvenile septic rats after Human Umbilical Cord Mesenchymal Stem Cells (HUMSCs) intervention for the first time, and screened the potential proteins and pathways of HUMSCs for therapeutic effect. The 4D proteome quantitative technique was used to quantitatively analyze the lung tissues of septic rats 24 hours (3 biological samples) and 24 hours after HUMSCs intervention (3 biological samples). A total of 213 proteins were identified as differentially expressed proteins, and 971 phosphorylation sites changed significantly. Based on the public database, we analyzed the functional enrichment of these proteins and phosphorylated proteins. In addition, Tenascin-C may be the key differential protein and ECM receptor interaction pathway may be the main signal pathway by using various algorithms to analyze the protein-protein interaction network. Phosphorylation analysis showed that tight junction pathway was closely related to immune inflammatory reaction, and EGFR interacted most, which may be the key differential phosphorylated protein. Finally, 123 conserved motifs of serine phosphorylation site (pS) and 17 conserved motifs of threonine (pT) phosphorylation sites were identified by motif analysis of phosphorylation sites. Results from proteomics and phosphorylated proteomics, the potential new therapeutic targets of HUMSCs in alleviating lung injury in juvenile septic rats were revealed.

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“…We recently demonstrated that curcumin treatment (5 μM) on human bone marrow–derived MSCs can enhance MMP13-mediated osteogenic differentiation, reduce MMP1 expression, and upregulate immunomodulatory gene IDO1 expression although it would inhibit MSC proliferation and migration in high concentration (10 μM or above) 130 . Treatment of a Chinese medicinal herbal formula, Danggui Buxue Tang (a mixture containing Astragali radix and Angelicae sinensis radix ), can increase the number of bone marrow stromal cells and promote cell adhesion and migration via focal adhesion and PI3K/Akt signaling pathway activation 131 . Moreover, treatment of Chinese medicinal herbal formula Du-Huo-Ji-Sheng-Tang and its active constituent Ligusticum chuanxiong hort can delay human MSC aging process by decreasing cell senescence and increasing its osteogenic activity 132 .…”

Section: Enhancement Of the Neuroprotective Effects For Adult Stem Ce...mentioning

confidence: 99%

Diversified Treatment Options of Adult Stem Cells for Optic Neuropathies

et al. 2022

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Optic neuropathies refer to a group of ocular disorders with abnormalities or dysfunction of the optic nerve, sharing a common pathophysiology of retinal ganglion cell (RGC) death and axonal loss. RGCs, as the retinal neurons in the central nervous system, show limited capacity in regeneration or recovery upon diseases or after injuries. Critically, there is still no effective clinical treatment to cure most types of optic neuropathies. Recently, stem cell therapy was proposed as a potential treatment strategy for optic neuropathies. Adult stem cells, including mesenchymal stem cells and hematopoietic stem cells, have been applied in clinical trials based on their neuroprotective properties. In this article, the applications of adult stem cells on different types of optic neuropathies and the related mechanisms will be reviewed. Research updates on the strategies to enhance the neuroprotective effects of human adult stem cells will be summarized. This review article aims to enlighten the research scientists on the diversified functions of adult stem cells and consideration of adult stem cells as a potential treatment for optic neuropathies in future clinical practices.

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Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro

Cited by 17 publications

References 32 publications

Isomer-Specific Effects of cis-9,trans-11- and trans-10,cis-12-CLA on Immune Regulation in Ruminal Epithelial Cells

Isomer-Specific Effects of cis-9,trans-11- and trans-10,cis-12-CLA on Immune Regulation in Ruminal Epithelial Cells

Proteomic and phosphorylated proteomic landscape of injured lung in juvenile septic rats with therapeutic application of umbilical cord mesenchymal stem cells

Diversified Treatment Options of Adult Stem Cells for Optic Neuropathies

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