Dangguishaoyao-San attenuates LPS-induced neuroinflammation via the TLRs/NF-κB signaling pathway

Ding, Ruirui; Chen, Wang; Guo, Cong-Ying; Liao, Wei-Tao; Yang, Xia; Liao, Feng-Er; Lin, Jiantao; Mei, Hanfang; Zeng, Yu

doi:10.1016/j.biopha.2018.05.108

Cited by 23 publications

(9 citation statements)

References 32 publications

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“…Subsequently, the free NF-κB dimer translocates to the nucleus and promoting the transcription of various pro-inflammatory enzymes (Mercurio and Manning, 1999;Ghosh and Hayden, 2008;Lappas et al, 2002;Ren et al, 2019;Cai et al, 2018). Many studies have found that inhibition of NF-ĸB pathway can effectively reduce LPS-induced inflammation (Fu et al, 2018;Ding et al, 2018). In this study, we also found that the expression of phosphorylated IκB and NF-κB were remarkably upregulated in LPS-stimulated group, which are consistent with previous studies.…”

Section: Discussionsupporting

confidence: 91%

Myricitrin blocks activation of NF-κB and MAPK signaling pathways to protect nigrostriatum neuron in LPS-stimulated mice

Yang

Liu

Xiao

et al. 2019

Journal of Neuroimmunology

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Myricitrin, a bioactive and natural flavonoids, is well known for its anti-inflammatory and antioxidant properties. However, the anti-neuroinflammation and possible mechanism has not been fully elucidated. Therefore, the present study was to investigate the possible mechanism of its neuroprotection and anti-neuroinflammation in the nigrostriatum of LPS-stimulated mice. The results showed that myricitrin improved neuron injury and raised the expressions of PSD-95 protein and TH protein in the nigrostriatum of LPS-stimulated mice. In addition, myricitrin decreased the production of pro-inflammatory factors including IL-1β, IL-6 and TNFα, decreased the level of chemokine MCP-1, and suppressed the expressions of COX-2 and iNOS. Meanwhile, myricitrin suppressed HMGB1, TLR4, and MyD88 expression in the nigrostriatum of LPS-stimulated mice. Furthermore, myricitrin inhibited NF-κB and MAPK signaling pathways activated by LPS. In conclusion, our studies suggest that myricitrin blocks activation of protects NF-κB and MAPK signaling pathways to nigrostiatum neuron from injury in LPS-stimulated mice and is beneficial to treatment nigrostriatum inflammation of PD.

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Section: Discussionsupporting

confidence: 91%

Myricitrin blocks activation of NF-κB and MAPK signaling pathways to protect nigrostriatum neuron in LPS-stimulated mice

Yang

Liu

Xiao

et al. 2019

Journal of Neuroimmunology

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“…The anti-inflammatory activity of the extracts was evaluated pretreating BV-2 cells with different concentrations (1-100 μ g mL −1 ) of the extracts for 24 h before exposing the cells to lipopolysaccharide (LPS) to induce inflammation ( Figure 8 ). LPS is the most widely used inflammatory mediator to activate microglial cells in vitro and triggers the proinflammatory signaling cascade [ 78 , 79 ]. LPS treatment significantly reduced cell viability with respect to control cells by ~40%.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Angeloni

Freschi

Marrazzo

et al. 2021

Oxidative Medicine and Cellular Longevity

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Spent coffee grounds (SCGs), waste products of coffee beverage production, are rich in organic compounds such as phenols. Different studies have demonstrated phenol beneficial effects in counteracting neurodegenerative diseases. These diseases are associated with oxidative stress and neuroinflammation, which initiates the degeneration of neurons by overactivating microglia. Unfortunately, to date, there are no pharmacological therapies to treat these pathologies. The aim of this study was to evaluate the phenolic content of 4 different SCG extracts and their ability to counteract oxidative stress and neuroinflammation. Caffeine and 5-O-caffeoylquinic acid were the most abundant compounds in all extracts, followed by 3-O-caffeoylquinic acid and 3,5-O-dicaffeoylquinic acid. The four extracts demonstrated a different ability to counteract oxidative stress and neuroinflammation in vitro. In particular, the methanol extract was the most effective in protecting neuron-like SH-SY5Y cells against H2O2-induced oxidative stress by upregulating endogenous antioxidant enzymes such as thioredoxin reductase, heme oxygenase 1, NADPH quinone oxidoreductase, and glutathione reductase. The water extract was the most effective in counteracting lipopolysaccharide-induced neuroinflammation in microglial BV-2 cells by strongly reducing the expression of proinflammatory mediators through the modulation of the TLR4/NF-κB pathway. On these bases, SCG extracts could represent valuable nutraceutical sources for the treatment of neurodegeneration.

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“…Figure 6 illustrated that lots of compounds in DSS act on the targets of NF-ĸB signaling pathway, indicating the potential AD therapeutic mechanism mediated by DSS. Recent in vitro study showed that DSS has a protective effect on neuroinflammation in lipopolysaccharide (LPS)-stimulated BV-2 microglia cells through the TLRs/NF-κB signaling pathway [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Systems pharmacology-based approach to investigate the mechanisms of Danggui-Shaoyao-san prescription for treatment of Alzheimer’s disease

Chen

et al. 2020

BMC Complement Med Ther

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Background Alzheimer’s disease (AD) is the most common cause of dementia in the elderly, characterized by a progressive and irreversible loss of memory and cognitive abilities. Currently, the prevention and treatment of AD still remains a huge challenge. As a traditional Chinese medicine (TCM) prescription, Danggui-Shaoyao-san decoction (DSS) has been demonstrated to be effective for alleviating AD symptoms in animal experiments and clinical applications. However, due to the complex components and biological actions, its underlying molecular mechanism and effective substances are not yet fully elucidated. Methods In this study, we firstly systematically reviewed and summarized the molecular effects of DSS against AD based on current literatures of in vivo studies. Furthermore, an integrated systems pharmacology framework was proposed to explore the novel anti-AD mechanisms of DSS and identify the main active components. We further developed a network-based predictive model for identifying the active anti-AD components of DSS by mapping the high-quality AD disease genes into the global drug-target network. Results We constructed a global drug-target network of DSS consisting 937 unique compounds and 490 targets by incorporating experimental and computationally predicted drug–target interactions (DTIs). Multi-level systems pharmacology analyses revealed that DSS may regulate multiple biological pathways related to AD pathogenesis, such as the oxidative stress and inflammatory reaction processes. We further conducted a network-based statistical model, drug-likeness analysis, human intestinal absorption (HIA) and blood-brain barrier (BBB) penetration prediction to uncover the key ani-AD ingredients in DSS. Finally, we highlighted 9 key ingredients and validated their synergistic role against AD through a subnetwork. Conclusion Overall, this study proposed an integrative systems pharmacology approach to disclose the therapeutic mechanisms of DSS against AD, which also provides novel in silico paradigm for investigating the effective substances of complex TCM prescription.

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Dangguishaoyao-San attenuates LPS-induced neuroinflammation via the TLRs/NF-κB signaling pathway

Abstract: This study demonstrated that DSS has a protective effect on neuroinflammation in LPS-induced BV-2 microglia cells through the TLRs/NF-κB signaling pathway.

Cited by 23 publications

References 32 publications

Myricitrin blocks activation of NF-κB and MAPK signaling pathways to protect nigrostriatum neuron in LPS-stimulated mice

Myricitrin blocks activation of NF-κB and MAPK signaling pathways to protect nigrostriatum neuron in LPS-stimulated mice

Antioxidant and Anti‐Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Systems pharmacology-based approach to investigate the mechanisms of Danggui-Shaoyao-san prescription for treatment of Alzheimer’s disease

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