Danhong injection enhances angiogenesis after myocardial infarction by activating MiR-126/ERK/VEGF pathway

Li, Si-Nai; Li, Ping; Liu, Weihong; Shang, Juju; Qiu, Sheng-Lei; Zhou, Mingxue; Liu, Hongxu

doi:10.1016/j.biopha.2019.109538

Cited by 39 publications

(30 citation statements)

References 37 publications

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“… 44 , 45 ERK1 and ERK2 are critical serine-threonine kinases of the MAPK family, and their important roles in regulating angiogenesis have been widely reported. 46 , 47 In this research, EOPF and perillaldehyde increased phosphorylation of ERK1/2 in sunitinib-treated HUVECs. EOPF and perillaldehyde might activate the MAPK pathways, promoting angiogenesis in HUVECs.…”

Section: Discussionmentioning

confidence: 55%

Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells

Zhou¹,

Dai²,

Peng³

et al. 2021

DDDT

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Purpose Perilla frutescens (L.) Britt., a traditional edible-medicinal herb in China, has been used to treat cardiovascular and cerebrovascular (cardio-cerebrovascular) diseases for thousands of years. However, knowledge of the mechanisms underlying the effects of essential oil from P. frutescens (EOPF) in the treatment of cardio-cerebrovascular diseases is lacking. The promotion of angiogenesis is beneficial in the treatment of ischemic cardio-cerebrovascular diseases. The current study investigated the pro-angiogenic role of EOPF and its main component perillaldehyde in sunitinib-injured transgenic Tg (flk1:EGFP) zebrafish embryos and human umbilical vein endothelial cells (HUVECs) for the first time. Materials and Methods The pro-angiogenic effects of EOPF and perillaldehyde were observed in vivo using transgenic Tg (flk1:EGFP) zebrafish embryos and in vitro using HUVECs. Cell viability, proliferation, migration, tube formation, and protein levels were detected by MTT, EdU staining, wound healing, transwell chamber, and Western blot assays, respectively. Results EOPF and perillaldehyde exerted a significant stimulatory effect on the formation of zebrafish intersegmental vessels (ISVs). Moreover, EOPF and perillaldehyde promoted proliferation, migration, and tube formation in sunitinib-treated HUVECs. Additionally, our findings uncovered that the pro-angiogenic effects of EOPF and perillaldehyde were mediated by increases in the expression ratios of p-ERK1/2 to ERK1/2 and Bcl-2 to Bax. Conclusion The present study is the first report to provide clear evidence that EOPF and perillaldehyde promote angiogenesis by stimulating repair of sunitinib-injured ISVs in zebrafish embryos and promoting proliferation, migration, and tube formation in sunitinib-injured HUVECs. The underlying mechanisms are related to increased p-ERK1/2 to ERK1/2 and Bcl-2 to Bax expression ratios. EOPF and perillaldehyde may be used in the treatment of cardio-cerebrovascular diseases, which is consistent with the traditional application of P. frutescens .

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Section: Discussionmentioning

confidence: 55%

Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells

Zhou¹,

Dai²,

Peng³

et al. 2021

DDDT

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“…42 Moreover, the essential developmental role of miR-126 has been confirmed due to its activation of the survival kinases ERK1/2 and Akt (VEGF signaling pathway) as well its role in increasing pro-angiogenic signaling. 43,44 Recent evidence has indicated that under diabetic conditions and the existence of increasing risk factors for type 2 diabetes, the expression level of miR-126 could decrease. 45 Furthermore, the recent investigation of miRNA patterns revealed that the upregulation of miR-210 could rescue the cardiac function after myocardial disease by increasing angiogenesis in the heart tissue.…”

Section: Discussionmentioning

confidence: 99%

The Combination Effect of Voluntary Exercise and Crocin on Angiogenic miRNAs in High-Fat Diet/Low-Dose STZ-Induced Type2 Diabetes in Rats: miR-126 and miR-210

2020

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Background: As one of the major complications of diabetes, cardiovascular disease might result in early death in people with diabetes. miR-126 and 210 expressions undergo alterations in cardiac disease and cause heart failure. Methods: Animals were divided into the 5 groups of control (Con), diabetes (Dia), diabeticcrocin (Dia-Cro), diabetic-voluntary exercise (Dia-Exe), and diabetic-crocin-voluntary exercise (Dia-Cro-Exe). Type 2 diabetes was induced by the use of a high-fat diet (4 weeks) and injection of streptozotocin (STZ) (i.p, 35 mg/kg). Animals received crocin orally (50 mg/kg), and voluntary exercise was performed alone or together for 8 weeks. QRT–PCR method was used to determine the levels of miR-210 and miR-126 in cardiac tissue. Results: The levels of miR-210 and miR-126 in the cardiac tissue augmented in both the crocin and voluntary exercise groups in comparison with the non-treated group (p<0.001). The use of combination therapy with exercise and crocin magnified their effects on miR-210 and miR-126 levels (p<0.001). Moreover, MiR-210 levels were lower in the crocin group compared to the exercise group (p<0.001). Conclusion: The results indicated that voluntary exercise combined with crocin might provide a novel therapeutic plan for cardiovascular disease through increasing miR-210 and miR-126 expression.

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“…Since myocardial cells are non-regenerative cells, the heart muscle cannot repair itself after myocardial infarction; thus, many scholars have focused on the treatment of myocardial infarction with stem cells [17][18][19]. Whether transplanting stem cells to heart tissue or using stem-cell patches to treat MI, oxygen is essential to improve the efficiency of vascularization among the affected tissues [20][21][22]. It is worth noting that hypoxia may also occur in the process of tissue regeneration; in particular, poor angiogenesis can result in cell death in heart tissue [23,24].…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic biomaterials: applications of photosynthesis in algae as oxygenerator in biomedical therapies

et al. 2021

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For most organisms, molecular oxygen is indispensable for normal physiological metabolism; in humans, prolonged hypoxia in tissues can induce many diseases, exemplified by cardiovascular disease, chronic wounds, and tissue necrosis. Therefore, the oxygen in our environment is vital for life. As a main source of oxygen in the natural world that transforms light energy into chemical energy and oxygen, photosynthesis has been widely studied in scientific research and used in production of food, fuel, and medicine. In recent years, photosynthesis has become more closely involved in biomedicine and has been widely used in photodynamic therapy, tissue regeneration, transplantation, and in treatment of specific diseases. This review summarizes innovative applications of photosynthesis in biomedical research and highlights the theory and implications of clinical treatment for specific diseases.

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Danhong injection enhances angiogenesis after myocardial infarction by activating MiR-126/ERK/VEGF pathway

Cited by 39 publications

References 37 publications

Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells

Pro-Angiogenic Effects of Essential Oil from Perilla frutescens and Its Main Component (Perillaldehyde) on Zebrafish Embryos and Human Umbilical Vein Endothelial Cells

The Combination Effect of Voluntary Exercise and Crocin on Angiogenic miRNAs in High-Fat Diet/Low-Dose STZ-Induced Type2 Diabetes in Rats: miR-126 and miR-210

Photosynthetic biomaterials: applications of photosynthesis in algae as oxygenerator in biomedical therapies

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