Danhong Injection Enhances the Therapeutic Efficacy of Mesenchymal Stem Cells in Myocardial Infarction by Promoting Angiogenesis

Chen, Jingrui; Wei, Jing; Huang, Yu‐Ting; Ma, Yuxian; Ni, Jingyu; Li, Min; Zhu, Yan; Gao, Xiumei; Fan, Guanwei

doi:10.3389/fphys.2018.00991

Cited by 20 publications

(10 citation statements)

References 67 publications

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“…Danhong injection (DHI), a traditional Chinese medicine, was extracted from Salviae miltiorrhizae Radix and Carthami tinctorii Flos. The main bioactive constituents in DHI include salvianic acid A, salvianic acid B, protocatechuic aldehyde, and rosmarinic acid [7]. In a previous study, DHI was demonstrated to exert many effects, such as anti-inflammatory, antioxidant, anticoagulatory, hypolipidemic, antiapoptotic, vasodilatory, and angiogenesis-promoting actions [8, 9], and DHI is widely used in the treatment of cerebral ischemia and cardiovascular diseases [10].…”

Section: Introductionmentioning

confidence: 99%

Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model

Wei

et al. 2019

Oxidative Medicine and Cellular Longevity

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Background. Among all the common complications that occur after abdominal surgery, intestinal adhesion is perhaps the most unpleasant one. However, current methods to treat and prevent intestinal adhesion are limited; thus, exploring new methods to prevent and treat intestinal adhesion is greatly needed. In this study, we demonstrated that Danhong injection (DHI) may be used as a promising method to prevent and treat intra-abdominal adhesion in a rat model. Materials and Methods. Forty-eight rats were randomly divided into six groups. Except for the sham-operated group, all rats underwent cecal abrasion to establish an adhesion model. After the operation, the rats in the DHI-treated groups received different doses of DHI via the tail vein daily, while the other group was treated with the same volume of saline solution. Seven days after the operation, all rats were sacrificed, and the degree of adhesion was evaluated by Nair’s scoring system. The extent of inflammation in the adhesion tissue was detected by HE staining and the expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β (TGF-β). The collagen deposition was assessed by Sirius red staining and α-SMA, MMP9, t-PA, and PAI-1 levels. Oxidative stress was indicated by the level of reactive oxygen species (ROS) in adhesion tissues and by immunohistochemical labeling of Nrf2. Furthermore, rat primary peritoneal mesothelial cells (RPMCs) were treated with H2O2 and DHI, and NF-κB phosphorylation was detected to illustrate the effect of DHI on oxidative stress. Results. The intra-abdominal adhesion scores were significantly decreased in the groups treated with a high dose of DHI compared with the control groups, and the degree of inflammation, fibrosis, and oxidative stress was also significantly decreased. DHI treatment significantly reduced the levels of TNF-α, TGF-β1, and PAI and increased the expression levels of MMP9, Nrf2, and t-PA in the adhesion tissues. ROS levels and NF-κB phosphorylation were significantly reduced in DHI-treated RPMCs compared with the control RPMCs. Conclusion. DHI alleviates the formation of postoperative intra-abdominal adhesions by inhibiting inflammation, collagen deposition, and oxidative stress in a rat model and may serve as a promising drug to prevent intra-abdominal adhesions.

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Section: Introductionmentioning

confidence: 99%

Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model

Wei

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…We proposed that THSWD could ameliorate the microenvironment after MI as it not only raised the expression of IGF-1 and bFGF but also enhanced angiogenesis and activated the PI3K/Akt signaling pathway. Recent evidence suggested that Danhong injection enhanced the residence of MSCs in the cardiac tissue, promoted angiogenesis, and reduced myocardial infarct size [45]. We also found that Guanxin Danshen formulation enhanced the effects of MSC transplantation for the treatment of MI [46].…”

Section: Discussionmentioning

confidence: 59%

Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction

Luo

Xiao

et al. 2019

Cardiology Research and Practice

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Cardiovascular disease has been established as a major cause of morbidity and mortality worldwide, resulting in a huge burden to patients, families, and society. Traditional Chinese Medicine (TCM) presents several advantages for the prevention and treatment of cardiovascular diseases including multitargets, multi-ingredients, fewer side effects, and low cost. In this study, a rat model of myocardial infarction (MI) was established by ligating the anterior descending branch of the left coronary artery, and the effect of the Taohong Siwu decoction (THSWD) on cardiac function was evaluated in MI rats. Following the intragastric administration of THSWD, the cardiac function was examined using echocardiography. The infarct size and collagen deposition in the infarct area were measured using Masson's trichrome staining, and the number of CD31- and α-SMA-positive blood vessels in the peri-infarct and infarct area was evaluated by immunofluorescent staining. The mRNA expression of bFGF, IGF-1, and HGF was detected using RT-PCR assay. Cell apoptosis in the infarcted area was assessed by TUNEL staining, and the p-Akt level was detected using the western blot assay. The mitochondrial ROS production was measured using MitoSOX staining, and mitochondrial dynamics and mitophagy were evaluated with western blotting 7 days after THSWD treatment. THSWD increased the ejection fraction (EF) and fractional shortening (FS) values in the rat hearts; however, no statistical difference was found between the THSWD and MI groups 4 weeks after treatment. Furthermore, THSWD significantly decreased the value of the left ventricular end-systolic volume (LVESV). Compared with the model group, THSWD significantly increased the expression of IGF-1 and bFGF, reduced collagen deposition, promoted angiogenesis, reduced cell apoptosis, and activated the PI3K/Akt signaling pathway. Notably, THSWD significantly decreased mitochondrial ROS production and Fis1 expression. No statistical differences were observed in the expression of mitochondrial LC3B and Mfn1 between the THSWD and control groups. In summary, THSWD may possess a beneficial effect on cardiac function by improving the local ischemic microenvironment and by decreasing mitochondrial fission after MI. Hence, this may present a promising auxiliary strategy in the treatment of ischemic cardiomyopathy such as MI.

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“…13 Several studies have demonstrated that after intravenous delivery, BM-MSCs migrate to the site of bone or cartilage damage, myocardial infarction, and ischemic cerebral injury. [14][15][16] Although MSCs were initially shown to promote tissue repair by trans-differentiation of specific cell types required to repair damaged tissue, current data suggest paracrine signaling as the primary mediator of the reparative effects of MSCs. 17 Practically, attracting more cells to a damaged tissue would enhance the recovery of the injured tissue because of the increase in paracrine factors.…”

Section: Introductionmentioning

confidence: 99%

<p>Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats</p>

Wei

Zhang

et al. 2020

IJN

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Introduction: Mesenchymal stem cells (MSCs) are a promising resource for tissue regeneration and repair. However, their clinical application is hindered by technical limitations related to MSC enrichment at the target sites. Methods: MSCs were labeled with magnetic Fe 3 O 4 nanoparticles (NPs). We analyzed the effects of NP on cell proliferation, stem cell characteristics, and cytokine secretion. Furthermore, we induced NP-labeled MSC migration with an external magnetic field toward laser-induced skin wounds in rats and evaluated the associated anti-inflammatory effects. Results: Fe 3 O 4 NP application did not adversely affect MSC characteristics. Moreover, Fe 3 O 4 NP-labeled MSCs presented increased anti-inflammatory cytokine and chemokine production compared with unlabeled MSCs. Furthermore, MSCs accumulated at the injury site and magnetic targeting promoted NP-labeled MSC migration toward burn injury sites in vivo. On day 7 following MSC injection, reduced inflammation and promoted angiogenesis were observed in the magnetically targeted MSC group. In addition, anti-inflammatory factors were upregulated, whereas pro-inflammatory factors were downregulated within the magnetically targeted MSC group compared with those in the PBS group. Conclusion: This study demonstrates that magnetically targeted MSCs contribute to cell migration to the site of skin injury, improve anti-inflammatory effects and enhance angiogenesis compared with MSC injection alone. Therefore, magnetically targeted MSC therapy may be an effective treatment approach for epithelial tissue injuries.

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Danhong Injection Enhances the Therapeutic Efficacy of Mesenchymal Stem Cells in Myocardial Infarction by Promoting Angiogenesis

Cited by 20 publications

References 67 publications

Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model

Danhong Injection Alleviates Postoperative Intra-abdominal Adhesion in a Rat Model

Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction

<p>Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats</p>

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