Danos causados por diferentes potenciais de inóculo de Aspergillus ochraceus no vigor de sementes de soja

Rocha, Fernando da Silva; Catão, Hugo César Rodrigues Moreira; Brandão, Antônio de Amorim; Gomes, Luíz Antônio Augusto

doi:10.5433/1679-0359.2014v35n6p2895

Cited by 9 publications

(9 citation statements)

References 18 publications

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“…When whole seeds and dissected fractions (tegument and embryo) were analyzed as for the concentration of C. the inoculum potential of pathogens can be accounted as a determinant factor for seed quality. The studies with soybean and Aspergillus ochraceus of Rocha et al (2014) corroborate the results of this work. They also noticed that, when the inoculum potential was high, seed quality and vigor were committed.…”

Section: Quantification Of C Lindemuthianum In Seed Fractions By Reasupporting

confidence: 88%

“…Based on this outcome, the cPCR technique can be applied for routine laboratory analyses, because it is easily reproducible and takes little time to provide reliable results. Similar findings of other authors endorse this recommendation (Chen et al, 2007;Gutiérrez et al, 2014;Mesquita et al, 1998;Pacheco et al, 2014).…”

Section: Specificity Sensitivity and Limit Of Detection Of The Convsupporting

confidence: 80%

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Molecular detection of Colletotrichum lindemuthianum in bean seed samples

2018

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Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.Index terms: Phaseolus vulgaris, anthracnose, PCR.Detecção molecular de Colletotrichum lindemuthianum em amostras de sementes de feijão RESUMO -Colletotrichum lindemuthianum é o agente causal da antracnose do feijoeiro, tendo a semente como uma forma de disseminação. O uso de sementes de feijão livres do patógeno é uma das estratégias de manejo, e o uso de técnicas de detecção precisas e rápidas torna-se indispensável para a análise sanitária prévia de sementes. Neste estudo, as técnicas de PCR convencional (cPCR) e PCR em tempo real (qPCR) foram utilizadas para a detecção e quantificação de C. lindemuthianum em amostras de sementes de feijão. Para isso, sementes foram inoculadas por meio de sua exposição às colônias do fungo por tempos variados, 0, 36, 72, 108, e 144 horas, cada período correspondendo a um potencial de inóculo, P0, P36, P72, P108 e P144. As sementes inoculadas foram misturadas com sementes sadias, obtendo-se incidências de 0,25% 0,50%, 1%, 10% e 100% de sementes para cada potencial de inóculo em amostras de 400 sementes. Tanto a cPRC como a qPCR foram eficazes na detecção do fungo. A partir das amostras de sementes com 10% de incidência e com P36, foi possível detectar o fungo por cPCR. Pela técnica de qPCR o patógeno foi detectado em amostras a partir da incidência de 0,25% no menor potencial de inóculo P36.Termos para indexação: Phaseolus vulgaris, antracnose, PCR.1

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Section: Quantification Of C Lindemuthianum In Seed Fractions By Reasupporting

confidence: 88%

Section: Specificity Sensitivity and Limit Of Detection Of The Convsupporting

confidence: 80%

Molecular detection of Colletotrichum lindemuthianum in bean seed samples

2018

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“…Aspergillus ochraceus on soybean seed surface caused high seed tissues degradation and reduced the seed vigor (ROCHA et al, 2014). The seeds are an effective source for dissemination and transmission of pathogens, often introducing new pathogens into disease free areas, and reducing the physiological quality of seed lots from these areas as well (NEERGAARD, 1979).…”

Section: R1mentioning

confidence: 99%

Physiological and phytosanitary quality of common bean seeds used by small growers in Minas Gerais state, Brazil

Lopes

Brandão

Araujo

et al. 2018

R. Agrogeoambiental

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The physiological and phytosanitary qualities of common bean seeds used on a small farming system depend on the cultural practices, drying system and storage conditions employed. The objective of this work was to evaluate eight lots of common bean seeds using germination, vigor and phytosanitary tests. The lots were produced in 2005 and 2006 in "Zona da Mata", Minas Gerais State, Brazil, and analyzed for moisture content, germination quality, first germination count, seedling vigor, seedling emergence, electric conductivity, seedling dry weight, seedling length, hypochlorite test and blotter test. The experimental design was completely randomized with four replications. The mean seed moisture content from all lots was 8.4 %. Four lots were classified as high vigor, one as intermediate vigor and three lots as low vigor. The first germination count and electrical conductivity tests were the best for evaluating bean seed physiological quality. All seed lots were infested by fungi, mainly Alternaria, Cladosporium and Fusarium.

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“…and attributed the reduced germination of cowpea seeds and the virulence of these fungi to enzymatic activity. Rocha et al (23) studied the physiological quality of soybean seeds at three inoculum levels and found 80% reduction in germination and seedling vigor and total tissue deterioration in seeds with incidence of up to or above 50% for A. ochraceus. In this study, the percentage of germinated seeds and elongated embryos treated with fungicide, compared to control, showed that the incidence of Curvularia sp., Penicillium spp.…”

Section: Resultsmentioning

confidence: 99%

“…The genus Curvularia is considered of weak pathogens but is common in several seeds of plants of economic importance (24). However, considerable fungal infestations can compromise seed germination and vigor (23). Furthermore, Curvularia pallecens, detected in the seeds of various cereals, produces a large quantity of amylase and its virulence is associated with greater production of this enzyme (9).…”

Section: Resultsmentioning

confidence: 99%

Health quality and germination of Butia capitata seeds treated with bactericide and fungicide

et al. 2016

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Propagation of "coquinho-azedo" (Butia capitata) is limited due to dormancy and high incidence of fungi in its seeds. The aim of this study was to evaluate the effect of treating "coquinho-azedo" seeds with different concentrations of bactericide and/or fungicide on the incidence of fungi and on the germination of seeds. Thus, B. capitata seeds were immersed in four doses of the bactericide kasugamycin (0, 1.5, 3.0 and 4.5 mL/L) and/or in four concentrations of the fungicide carbendazim+ thiram (0, 2.92, 5.84 e 8.75 mL/ Kg), totaling 16 treatments. After the treatments, the operculum was removed from the seeds, which were placed on trays containing autoclaved sand, covered with transparent plastic film and kept in the nursery. In the germination test, treatments were distributed in randomized block design, factorial arrangement Keywords: Carbendazim, thiram, "coquinho-azedo", fungus, incidence. ABSTRACTof 4x4 (bactericidal doses x fungicide doses), with four replicates of 50 seeds per plot. For the test of seed health quality, the Blotter test was employed in completely randomized design, using 20 replicates and 10 seeds. Health quality and germination were evaluated at 15 and 40 days after the treatment of seeds, respectively. There was no interaction between the bactericide and the fungicide on germination and control of fungi associated with seeds. However, there was an effect of fungicide concentrations on the evaluated parameters. We observed a negative correlation between the concentrations of the fungicide carbendazim + thiram and fungal incidence, and a positive correlation between the fungicide concentrations and seed germination. Therefore, fungicide treatment reduces the fungal incidence and provides better germination of B. capitata seeds. RESUMOA propagação do coquinho-azedo (Butia capitata) é limitada devido a dormência e a alta incidência de fungos em suas sementes. O objetivo deste trabalho foi avaliar o efeito do tratamento de sementes de coquinho-azedo com diferentes concentrações de bactericida e/ou fungicida na incidência de fungos e na germinação das sementes. Para isto, sementes de coquinho-azedo foram imersas em quatro doses do bactericida kasugamicina (0, 1.5, 3.0 e 4.5 mL/L) e/ou em quatro concentrações do fungicida carbendazim + thiram (0, 2.92, 5.84 e 8.75 mL/Kg), totalizando 16 tratamentos. Após o tratamento, retirou-se o opérculo das sementes, as quais foram colocadas em bandejas contendo areia autoclavada, revestidas com filme plástico transparente, e mantidas em viveiro. No teste de germinação, os tratamentos foram distribuídos em delineamento de blocos casualizados em esquema fatorial 4 x 4 (doses Lacerda, V. R.; Mendes, D.S.T.; Rocha, F.S.; Fernandes, M.F.G.; Lopes, P.S.N.; Civil, N.; Catão, H.C.R.M. Qualidade sanitária e germinação de sementes de Butia capitata tratadas com bactericida e fungicida. Summa Phytopathologica, v.42, n.4, p.303-307, 2016. do bactericida e doses do fungicida), com 4 repetições de 50 sementes por parcela. Para o teste de sanidade foi empregado o méto...

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Danos causados por diferentes potenciais de inóculo de Aspergillus ochraceus no vigor de sementes de soja

Cited by 9 publications

References 18 publications

Molecular detection of Colletotrichum lindemuthianum in bean seed samples

Molecular detection of Colletotrichum lindemuthianum in bean seed samples

Physiological and phytosanitary quality of common bean seeds used by small growers in Minas Gerais state, Brazil

Health quality and germination of Butia capitata seeds treated with bactericide and fungicide

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