Danthron ameliorates obesity and MAFLD through activating the interplay between PPARα/RXRα heterodimer and adiponectin receptor 2

Ma, Chuanrui; Wang, Zhongyan; Xia, Ronglin; Wei, Lingling; Zhang, Chao; Zhang, Jing; Zhao, Linna; Wu, Han; Kang, Lin; Yang, Shu

doi:10.1016/j.biopha.2021.111344

Cited by 14 publications

(11 citation statements)

References 58 publications

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“…To further investigate the effects of perillartine on fat accumulation in vivo , mice were fed a normal diet or a high-fat diet supplemented with perillartine, with normal and high-fat diets serving as controls. 25–27 Compared with the high-fat diet, which showed rapid weight gain, the addition of perillartine could slow down the rate of weight gain in a dose-dependent manner, and there was a significant difference in body weight between the groups after 6 weeks of the high-fat diet. However, no difference in body weight was observed between the three groups given the normal diet (Fig.…”

Section: Resultsmentioning

confidence: 99%

Perillartine protects against metabolic associated fatty liver in high-fat diet-induced obese mice

Xiao

et al. 2023

Food Funct.

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Section: Resultsmentioning

confidence: 99%

Perillartine protects against metabolic associated fatty liver in high-fat diet-induced obese mice

Xiao

et al. 2023

Food Funct.

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“…To identify small molecules facilitating the induction of NCCs to differentiate into CEC destined cells, we built a chemical library of 30 small molecules that targeted almost all the key pathways of stem cell differentiation and corneal development ( Table 1 ). The final concentration of each compound added to the medium was based on previously reported data and testing for the ED50s in the NCCs via Cell Counting Kit-8 (CCK-8) assay ( Miner et al, 2003 ; Bouzakri et al, 2004 ; Moreno et al, 2008 ; Johnson et al, 2012 ; Yap et al, 2012 ; Cho et al, 2013 ; Han et al, 2014 ; Schelleman et al, 2014 ; Kampa-Schittenhelm et al, 2017 ; Pinkosky et al, 2020 ; Zhang et al, 2020 ; Gampala et al, 2021 ; Ma et al, 2021 ). For initial screening, NCCs derived from dual-SMAD inhibition of hiPSCs for 6 days were cultured in a 24-well plate and treated with small molecules for another 3 days.…”

Section: Resultsmentioning

confidence: 99%

Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells

Chen

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Purpose: Corneal endothelial cells (CECs) serve as a barrier and foothold for the corneal stroma to maintain the function and transparency of the cornea. Loss of CECs during aging or disease states leads to blindness, and cell replacement therapy using either donated or artificially differentiated CECs remains the only curative approach.Methods: Human induced pluripotent stem cells (hiPSCs) that were cultured in chemically defined medium were induced with dual-SMAD inhibition to differentiate into neural crest cells (NCCs). A small-molecule library was screened to differentiate the NCCs into corneal endothelial-like cells. The characteristics of these cells were identified with real-time PCR and immunofluorescence. Western blotting was applied to detect the signaling pathways and key factors regulated by the small molecules.Results: We developed an effective protocol to differentiate hiPSCs into CECs with defined small molecules. The hiPSC-CECs were characterized by ZO-1, AQP1, Vimentin and Na+/K+-ATPase. Based on our small-molecule screen, we identified a small-molecule combination, A769662 and AT13148, that enabled the most efficient production of CECs. The combination of A769662 and AT13148 upregulated the PKA/AKT signaling pathway, FOXO1 and PITX2 to promote the conversion of NCCs to CECs.Conclusion: We established an efficient small molecule-based method to differentiate hiPSCs into corneal endothelial-like cells, which might facilitate drug discovery and the development of cell-based therapies for corneal diseases.

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“…Since Nisch regulates the expression of ACSL3, PPARα, and CHREBP, activation of PPARα/AMPK expression of Nisch may be a novel approach to inhibit obesity and hepatic steatosis, and thus, Nisch may play an important role in obesity. Activation of PPARα leads to activation of downstream enzymes important in fat metabolism and glucose homeostasis [34], which can help diabetic obese patients. Additionally, a study indicates that PPARα increases adiponectin secretion that leads to increased hepatic fatty acid oxidation, which inhibits obesity-induced fatty liver [35].…”

Section: Discussionmentioning

confidence: 99%

Nischarin Deletion Reduces Oxidative Metabolism and Overall ATP: A Study Using a Novel NISCHΔ5-6 Knockout Mouse Model

Nguyen

Yousefi

Okpechi

et al. 2022

IJMS

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Nischarin (Nisch) is a cytosolic scaffolding protein that harbors tumor-suppressor-like characteristics. Previous studies have shown that Nisch functions as a scaffolding protein and regulates multiple biological activities. In the current study, we prepared a complete Nisch knockout model, for the first time, by deletion of exons 5 and 6. This knockout model was confirmed by Qrt–PCR and Western blotting with products from mouse embryonic fibroblast (MEF) cells. Embryos and adult mice of knockouts are significantly smaller than their wild-type counterparts. Deletion of Nisch enhanced cell migration, as demonstrated by wound type and transwell migration assays. Since the animals were small in size, we investigated Nisch’s effect on metabolism by conducting several assays using the Seahorse analyzer system. These data indicate that Nisch null cells have lower oxygen consumption rates, lower ATP production, and lower levels of proton leak. We examined the expression of 15 genes involved in lipid and fat metabolism, as well as cell growth, and noted a significant increase in expression for many genes in Nischarin null animals. In summary, our results show that Nischarin plays an important physiological role in metabolic homeostasis.

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Danthron ameliorates obesity and MAFLD through activating the interplay between PPARα/RXRα heterodimer and adiponectin receptor 2

Cited by 14 publications

References 58 publications

Perillartine protects against metabolic associated fatty liver in high-fat diet-induced obese mice

Perillartine protects against metabolic associated fatty liver in high-fat diet-induced obese mice

Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells

Nischarin Deletion Reduces Oxidative Metabolism and Overall ATP: A Study Using a Novel NISCHΔ5-6 Knockout Mouse Model

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