DAPK promotor methylation is an early event in colorectal carcinogenesis

Mittag, Falk; Kuester, Doerthe; Vieth, Michael; Peters, Brigitte; Stolte, Bernd; Roessner, Albert; Schneider‐Stock, Regine

doi:10.1016/j.canlet.2005.08.034

Cited by 58 publications

(37 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous studies report a significant correlation of alteration of protein expressions in tumor surrounding benign tissues of several cancers with different stages of metastatic disease or local progression. In their molecular behavior the adjacent benign tissues seem to act like malignant tissue [18][19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Alteration of bone sialoprotein expression in osseous metastasized renal cell carcinomas and the tumor surrounding tissue

Mittag

Hennenlotter²,

Minkley³

et al. 2011

Clin Exp Metastasis

View full text Add to dashboard Cite

Bone sialoprotein (BSP) regulates bone metabolism by directly influencing the activity of osteoblasts and osteoclasts. A significant correlation between the tissue expression of BSP in tumors and the occurrence of bone metastases was found in different cancers. Aim of this study was to identify the BSP expression in renal cell carcinomas (RCC) according to their stage of metastatic disease. Tissue samples of patients with RCC who underwent partial resection or nephrectomy were separated into three groups, each with 10 patients showing either no metastases (group I), only soft tissue metastases (group II) or bone metastases (group III) at date of surgery. Immunohistochemical analysis of BSP expression in tumor tissue and corresponding renal parenchyma was performed and evaluated with an established semiquantitative scoring system. BSP expression was detected both in tumor tissue and renal parenchyma. Concerning the expression in malignant tissue, no significant difference could be found between the three groups whereas the corresponding renal parenchyma showed a staining score of 164, 198 and 224 for group I, II and III (P = 0.07). RCC staged T3 showed only a little higher BSP expression than those staged T1/2 (P < 0.21), while the corresponding parenchyma of T3 tumors showed significantly higher expressions (P = 0.02). This pilot study revealed a correlation between expression of BSP and tumor staging and type of metastases, especially for osseous metastases in RCC. Alternation of BSP expression could be detected particularly in renal parenchyma and linked to the type of metastases.

show abstract

Section: Discussionmentioning

confidence: 99%

Alteration of bone sialoprotein expression in osseous metastasized renal cell carcinomas and the tumor surrounding tissue

Mittag

Hennenlotter²,

Minkley³

et al. 2011

Clin Exp Metastasis

View full text Add to dashboard Cite

show abstract

“…Loss of DAPK activity leads to protection from interferon-␥-induced cell death, and overexpression of DAPK leads to apoptosis (30). The inactivation of DAPK by promoter hypermethylation has also been suggested to play an important role in the early steps of tumor progression to colorectal carcinoma (32). Thus, downregulation of calmodulin may contribute to colorectal tumorigenesis via inactivation of DAPK.…”

Section: Discussionmentioning

confidence: 99%

Identification of Early Intestinal Neoplasia Protein Biomarkers Using Laser Capture Microdissection and MALDI MS

Xu¹,

Beauchamp

et al. 2009

Molecular & Cellular Proteomics

View full text Add to dashboard Cite

Obtaining protein profiles from a homogeneous cell population in tissues can significantly improve our capability in protein biomarker discovery. In this study, unique protein profiles from the top and bottom sections of mouse crypts and Apc Min؉/؊ adenomas were obtained using laser capture microdissection (LCM) combined with MALDI MS. Statistically significant protein peaks with differential expression were selected, and a set of novel protein biomarkers were identified. Immunohistochemistry was performed to confirm the differentially expressed protein biomarkers found by LCM combined with MALDI MS. To validate the relevance of the findings in human colorectal cancer (CRC), S100A8 was further confirmed in human CRC using immunohistochemistry. In addition, S100A8 was found to have an increased expression at different human CRC stages (Duke's A-D) compared with controls at both protein (n ‫؍‬ 168 cases) and RNA (n ‫؍‬ 215 cases) levels. Overall LCM combined with MALDI MS is a promising method to identify intestinal protein biomarkers from minute amounts of tissue. The novel protein biomarkers identified from the top and bottom crypts will increase our knowledge of the specific protein changes taking place during cell migration from the crypt bottom to top. In addition, the identified cancer protein biomarkers will aid in the exploration of colorectal tumorigenesis mechanisms as well as in the advancement of molecularly based diagnosis of colorectal cancer. Molecular & Cellular Proteomics 8:936 -945, 2009.

show abstract

“…(ii) Second, the cancer cell can silence or lose a downstream proapoptotic partner of the receptor. As an example, death-associated protein kinase 1(figure 3B), a proapoptotic partner of UNC5B, is silenced by hypermethylation of its promoter in CRC 80. (iii) Third, an autocrine production of ligand can be selected by the tumour, that is, the cancer cell or the associated stroma, and this would keep the receptor fooled in an occupied state (figure 2A).…”

Section: Therapeutic Perspectives Linked To Dependence Receptorsmentioning

confidence: 99%

Dependence receptors and colorectal cancer

Mehlen

Tauszig-Delamasure

2014

Gut

View full text Add to dashboard Cite

The research on colorectal cancer (CRC) biology has been leading the oncology field since the early 1990s. The search for genetic alterations has allowed the identification of the main tumour suppressors or oncogenes. Recent work obtained in CRC has unexpectedly proposed the existence of novel category of tumour suppressors, the so-called 'dependence receptors'. These transmembrane receptors behave as Dr Jekyll and Mr Hyde with two opposite sides: they induce a positive signalling (survival, proliferation, differentiation) in presence of their ligand, but are not inactive in the absence of their ligand and rather trigger apoptosis when unbound. This trait confers them a conditional tumour suppressor activity: they eliminate cells that grow abnormally in an environment offering a limited quantity of ligand. This review will describe how receptors such as deleted in colorectal carcinoma (DCC), uncoordinated 5 (UNC5), rearranged during transfection (RET) or TrkC constrain CRC progression and how this dependence receptor paradigm may open up therapeutical perspectives.

show abstract

DAPK promotor methylation is an early event in colorectal carcinogenesis

Cited by 58 publications

References 17 publications

Alteration of bone sialoprotein expression in osseous metastasized renal cell carcinomas and the tumor surrounding tissue

Alteration of bone sialoprotein expression in osseous metastasized renal cell carcinomas and the tumor surrounding tissue

Identification of Early Intestinal Neoplasia Protein Biomarkers Using Laser Capture Microdissection and MALDI MS

Dependence receptors and colorectal cancer

Contact Info

Product

Resources

About