Daptomycin and Rifampin Alone and in Combination Prevent Vascular Graft Biofilm Formation and Emergence of Antibiotic Resistance in a Subcutaneous Rat Pouch Model of Staphylococcal Infection

Cirioni, Oscar; Mocchegiani, Federico; Ghiselli, Roberto; Silvestri, Carmela; Gabrielli, Eleonora; Marchionni, Elisa; Orlando, Fiorenza; Nicolini, Daniele; Risaliti, Andrea; Giacometti, Andrea

doi:10.1016/j.ejvs.2010.08.009

Cited by 65 publications

(48 citation statements)

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“…Unlike other studies 8,12,13 , we demonstrated that rifampicin alone is worse than vancomycin for inhibiting staphylococci embedded in biofilm. On the other hand, rifampicin in combination with vancomycin at 15mg/l inhibited bacterial grown in biofilm and, therefore, improved vancomycin activity, because of rifampicin's better biofilm penetration 10,20 .…”

Section: Discussioncontrasting

confidence: 44%

“…Otherwise, in our setting, vancomycin is preferable as antimicrobial coverage, and rifampicin is unusually prescribed. Because studies have demonstrated that rifampicin in combination with other drugs might be more effective 12,13 despite contradictory results 15 , we decided to investigate rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA.…”

Section: Discussionmentioning

confidence: 99%

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Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Reiter

Sambrano

Villa

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Introduction: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. Methods: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. Results: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log 10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). Conclusions: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin.

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Section: Discussioncontrasting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Reiter

Sambrano

Villa

et al. 2012

Rev. Soc. Bras. Med. Trop.

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“…The combination of DPT with rifampin appears useful for treating biofilm, as a consequence of a synergistic mechanism, as showed by Cirioni et al [36] Indeed, authors found that the combination of these molecules had higher efficacy than that of each single compound for treating a rat model of Staphylococcal infection. Authors suggest that the positive interaction between DPT and rifampin could be explained by the mechanism of action of DPT, which opens channels in the cell membrane, promoting entry of hydrophobic drugs such as rifampin.…”

Section: Daptomycin Lock Therapymentioning

confidence: 84%

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

Vassallo

Dunais

Roger³

2015

Infection

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“…As is the case for many antibiotics, daptomycin has been shown to exhibit a significant bactericidal activity against planktonic cells (7)(8)(9). However, the eradication of adherent bacteria is rarely achieved despite the large number of in vitro and animal studies in which daptomycin activity was evaluated (10)(11)(12)(13)(14)(15)(16). Besides the results of the literature that appear controversial (17), direct comparison between studies is not directly possible, since the biofilm growth and treatment protocols used differ greatly.…”

mentioning

confidence: 93%

New Insight into Daptomycin Bioavailability and Localization in Staphylococcus aureus Biofilms by Dynamic Fluorescence Imaging

Boudjemaa

Briandet

Revest

et al. 2016

Antimicrob Agents Chemother

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Staphylococcus aureus is one of the most frequent pathogens responsible for biofilm-associated infections (BAI), and the choice of antibiotics to treat these infections remains a challenge for the medical community. In particular, daptomycin has been reported to fail against implant-associated S. aureus infections in clinical practice, while its association with rifampin remains a good candidate for BAI treatment. To improve our understanding of such resistance/tolerance toward daptomycin, we took advantage of the dynamic fluorescence imaging tools (time-lapse imaging and fluorescence recovery after photobleaching [FRAP]) to locally and accurately assess the antibiotic diffusion reaction in methicillin-susceptible and methicillin-resistant S. aureus biofilms. To provide a realistic representation of daptomycin action, we optimized an in vitro model built on the basis of our recently published in vivo mouse model of prosthetic vascular graft infections. We demonstrated that at therapeutic concentrations, daptomycin was inefficient in eradicating biofilms, while the matrix was not a shield to antibiotic diffusion and to its interaction with its bacterial target. In the presence of rifampin, daptomycin was still present in the vicinity of the bacterial cells, allowing prevention of the emergence of rifampin-resistant mutants. Conclusions derived from this study strongly suggest that S. aureus biofilm resistance/tolerance toward daptomycin may be more likely to be related to a physiological change involving structural modifications of the membrane, which is a strain-dependent process. Staphylococcus aureus is a Gram-positive bacterial species shown to be the most frequent cause of biofilm-associated infections (BAI) (1) and one of the major causes of morbidity and mortality in hospitals and communities (2). Unlike planktonic cells, biofilms exhibit specific phenotypic traits allowing them to resist host defenses and antibiotic treatments (3), which frequently leads to chronic infections such as endocarditis, sinusitis, and osteomyelitis and also to implant-associated infections (4).Among the most recent clinically used antibiotics, daptomycin is a cyclic lipopeptide approved for the treatment of serious staphylococcal infections such as bacteremia and implant-related infections (5). Daptomycin is a calcium-dependent antibiotic that acts by insertion into the Gram-positive cytoplasmic membranes where it forms oligomeric pores, causing potassium ion leakage and subsequent membrane depolarization, leading ultimately to cell death (6). As is the case for many antibiotics, daptomycin has been shown to exhibit a significant bactericidal activity against planktonic cells (7-9). However, the eradication of adherent bacteria is rarely achieved despite the large number of in vitro and animal studies in which daptomycin activity was evaluated (10-16). Besides the results of the literature that appear controversial (17), direct comparison between studies is not directly possible, since the biofilm growth and treatment protocols...

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Daptomycin and Rifampin Alone and in Combination Prevent Vascular Graft Biofilm Formation and Emergence of Antibiotic Resistance in a Subcutaneous Rat Pouch Model of Staphylococcal Infection

Abstract: Daptomycin is an important candidate for prevention of staphylococcal biofilm-related infection and rifampin could serve as an interesting anti-staphylococcal antibiotic enhancer.

Cited by 65 publications

References 32 publications

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Antimicrobial lock therapy in central-line associated bloodstream infections: a systematic review

New Insight into Daptomycin Bioavailability and Localization in Staphylococcus aureus Biofilms by Dynamic Fluorescence Imaging

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