Daptomycin and vancomycin non-susceptible methicillin-resistant Staphylococcus aureus clonal lineages from bloodstream infection in a Brazilian teaching hospital

Damasco, Andreia Paredes; Costa, Thaina Miranda da; Morgado, Priscylla Guimarães Migueres; Guimarães, Lorrayne Cardoso; Cavalcante, Fátima Gonçalves; Nouér, Simone Aranha; Santos, Kátia Regina Netto dos

doi:10.1016/j.bjid.2019.03.003

Cited by 13 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over recent years, the substitution of well-established MRSA lineages has been described in hospital environments [7,8]. In Brazilian hospitals, the previously predominant MRSA lineage, known as the Brazilian Endemic Clone (BEC/ST239) has been fully replaced by the New York/Japan lineage among BSI isolates (USA100/ST5 or ST105) [9][10][11]. Lately, our group also detected the emergence of USA1100/ST30, a CA-MRSA lineage in Brazilian hospital settings [8,10].…”

Section: Introductionmentioning

confidence: 54%

“…According to Kourtis et al [32] CA-MRSA infections provide a reservoir that contributes to the incidence of healthcare-associated diseases and fuels transmission both within and outside healthcare settings. Our group has been carrying out a surveillance of S. aureus BSI in this hospital (HUCFF) for years and, although we have described the presence of community S. aureus lineages in this hospital since 2009 [8,10,[33][34][35] we had not yet seen the presence and spread of USA300/ST8/SCCme-cIVa. Some Brazilian studies have shown the presence of CA-MRSA isolates in hospitals recovered from different clinical sources, including occasionally USA300/ ST8/SCCmecIV isolates [36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

“…Isolates with four or fewer bands of difference or minimum of 80% similarity were classified as belonging to the same genotype [ 25 ]. The clonal lineages were defined by comparison with national [ 10 ] and international clones [ 26 ]. Representative isolates from each genotype, identified in PFGE, underwent Multilocus Sequence Typing (MLST) [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

“…In Brazilian hospitals, the previously predominant MRSA lineage, known as the Brazilian Endemic Clone (BEC/ST239) has been fully replaced by the New York/Japan lineage among BSI isolates (USA100/ST5 or ST105) [9][10][11]. Lately, our group also detected the emergence of USA1100/ST30, a CA-MRSA lineage in Brazilian hospital settings [8,10]. Curiously, in Brazil, the pandemic clone USA300/ST8/SCCmecIVa and the USA300-Latin American Variant (LV)/SCCmecIVc-e lineages have remained rare [11].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pandemic clone USA300 in a Brazilian hospital: detection of an emergent lineage among methicillin-resistant Staphylococcus aureus isolates from bloodstream infections

Augusto

Fernandes

Oliveira

et al. 2022

Antimicrob Resist Infect Control

Self Cite

View full text Add to dashboard Cite

Background Staphylococcus aureus is one of the leading causes of bloodstream infections (BSI) worldwide. In Brazil, the hospital-acquired methicillin-resistant S. aureus USA100/SCCmecII lineage replaced the previously well-established clones. However, the emergence of community-associated (CA) MRSA lineages among hospitalized patients is an increasing issue. Methods Consecutive S. aureus isolates recovered from BSI episodes of patients admitted between January 2016 and December 2018 in a Brazilian teaching hospital were tested for antimicrobial resistance, their genotypic features were characterized, and the clinical characteristics of the patients were evaluated. Results A total of 123 S. aureus isolates were recovered from 113 patients. All isolates were susceptible to linezolid, teicoplanin and vancomycin and 13.8% were not susceptible to daptomycin. Vancomycin MIC50 and MIC90 of 2 mg/L were found for both MRSA and MSSA isolates. The MRSA isolation rate was 30.1% (37/123), and 51.4% of them carried the SCCmec type II, followed by SCCmecIV (40.5%). Among the 37 MRSA isolates, the main lineages found were USA100/SCCmecII/ST5 and ST105 (53.7%) and USA800/ST5/SCCmecIV (18.9%). Surprisingly, six (16%) CA-MRSA isolates, belonging to USA300/ST8/SCCmecIVa that carried PVL genes and the ACME cassette type I, were detected. These six patients with USA300 BSI had severe comorbidities, including cancer, and most had a Charlson score ≥ 5; furthermore, they were in wards attended by the same health professionals. MRSA isolates were associated with hospital acquired infections (p = 0.02) in more elderly patients (p = 0.03) and those diagnosed with hematologic cancer (p = 0.04). Among patients diagnosed with MRSA BSI, 19 (54.3%) died. Conclusions The pandemic MRSA USA300 was detected for the first time in the Brazilian teaching hospital under study, and its cross-transmission most probably occurred between patients with BSI. This lineage may already be circulating among other Brazilian hospitals, which highlights the importance of carrying out surveillance programs to fight multidrug resistant and hypervirulent isolates.

show abstract

Section: Introductionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pandemic clone USA300 in a Brazilian hospital: detection of an emergent lineage among methicillin-resistant Staphylococcus aureus isolates from bloodstream infections

Augusto

Fernandes

Oliveira

et al. 2022

Antimicrob Resist Infect Control

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous published bacterial isolates of S. lugdunensis (541s and 546s) and S. aureus (63a, 1636a, 1176a and 1348a) [9,39,40] were selected for the study. These isolates belonged to the Laboratory of Hospital Infection collection at the Federal University of Rio de Janeiro.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Lugdulysin of Staphylococcus lugdunensis, a metalloprotease that inhibits and disrupts protein biofilm of Staphylococcus aureus

Martínez

Vázquez

Takeyama

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background Staphylococcus lugdunensis is a commensal skin microorganism that, unlike other coagulase-negative staphylococci, presents increasing clinical importance. This species yields a metalloprotease called lugdulysin that may contribute to its higher degree of virulence. This study aimed to determine the biochemical characterization of the lugdulysin produced by S. lugdunensis clinical isolates and investigate its effect on the formation and disruption of biofilm of Staphylococcus aureus isolates. The protease was isolated and characterized for its optimal pH and temperature, activity in the presence of inhibitors and enzymatic kinetics. The influence of metal cofactor supplementation on proteolysis was also evaluated, with and without inhibitors. Finally, the protease capacity to inhibit and disrupt biofilms of different S. aureus lineages and biofilm matrix was analyzed. Results The protease optimal pH and temperature were 7.0 and 37° C, respectively. EDTA inhibited the protease, and the activity was not recovered by divalent ion supplementation. In addition, divalent ions did not change enzymatic activity without inhibitors, which was stable for up to 3 hours. Its structure was determined via homology modelling. The protease significantly inhibited the formation and disrupted established biofilms of S. aureus isolates with protein biofilm. Conclusions This study confirmed features of the lugdulysin metalloprotease and showed that this S. lugdunensis virulence factor may be a new competition mechanism and/or modulation of the staphylococcal biofilm.

show abstract

Antibiotic resistance and biofilm synthesis genes in airborne Staphylococcus in commercial aircraft cabins

et al. 2021

View full text Add to dashboard Cite

Daptomycin and vancomycin non-susceptible methicillin-resistant Staphylococcus aureus clonal lineages from bloodstream infection in a Brazilian teaching hospital

Cited by 13 publications

References 13 publications

Pandemic clone USA300 in a Brazilian hospital: detection of an emergent lineage among methicillin-resistant Staphylococcus aureus isolates from bloodstream infections

Pandemic clone USA300 in a Brazilian hospital: detection of an emergent lineage among methicillin-resistant Staphylococcus aureus isolates from bloodstream infections

Lugdulysin of Staphylococcus lugdunensis, a metalloprotease that inhibits and disrupts protein biofilm of Staphylococcus aureus

Antibiotic resistance and biofilm synthesis genes in airborne Staphylococcus in commercial aircraft cabins

Contact Info

Product

Resources

About