Dark-based Optical Sectioning assists Background Removal in Fluorescence Microscopy
Ruijie Cao,
Yaning Li,
Wenyi Wang
et al.
Abstract:A fundamental challenge in fluorescence microscopy is the defocused background caused by scattering light, optical aberration, or limited axial resolution. Severe defocus backgrounds will submerge the in-focus information and cause artifacts in the following processing. Here, we leverage a priori knowledge about dark channels of biological structures and dual frequency separation to develop a single-frame defocus removal algorithm. It stably improves the signal-to-background ratio and structural similarity ind… Show more
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