Darkground microscopy of subgingival plaque from the top to the bottom of the periodontal pocket

Omar, A. A.; Newman, H. N.; Bulman, J S; Osborn, Jeffrey S.

doi:10.1111/j.1365-2710.1992.tb00794.x

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…Earlier cross-sectional studies using dark field microscopy for the evaluation of the % distribution of subgingival plaque morphotypes have demonstrated that subgingivai spirochetes and motile bacteria residing at the most apical level of the periodontal pocket, are associated with periodontai disease (Listgarten & Hellden !978, Lindhe et al 1980, Listgarten & Levin 1981, Armitage et al 1982, Tanner et al, 1984, Zappa et al 1986, Omar et al 1990. Some studies (Gusberti et al 1983, Ma-shimo et al 1983, Sandholm et al, 1989 have been published on the distribution of spirochetes and motile bacteria in the subgingiva] plaque of poorly controlled insulin-dependent diabetics (PIDD) and controlled insuUn-dependent diabetics (CIDD).…”

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confidence: 99%

Dark field microscopy of the subgingival microflora in insulin‐dependent diabetics

Seppala

Ainamo

1996

J Clinic Periodontology

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The subgingival microflora was assessed by means of dark field microscopy in 106 pockets of 47 subjects with long-term insulin-dependent diabetes mellitus (IDD). The microbiota of 55 healthy sulci (probing depth < 4 mm) and 51 periodontally diseased (probing depth > or = 4 and 6 mm) pockets were analyzed. The mean duration of the IDD of the diabetic subjects was 23.7 years (range from 10 to 41 years). The diabetic patients, aged 30-65 years, were under medical treatment at the III Department of Medicine, University of Helsinki Central Hospital, and at 2 clinics of the Helsinki Health Centre. Based upon their long-term medical records, 26 subjects were assessed to have poorly controlled insulin-dependent diabetes mellitus (PIDD) and 21 had controlled insulin-dependent diabetes (CIDD). The PIDD subjects exhibited higher mean blood glucose levels (12.9 +/- 4.6 mmol/l, mean +/- S.D.) than the CIDD subjects (7.9 +/- 3.6 mmol/l) (p < 0.001, t-test). The mean glycosylated hemoglobin HBA1 (HBA1c) levels were 11.2 +/- 4.6% (10.3 +/- 1.2%) and 8.8 +/- 1.8% (7.7 +/- 1.4%) for PIDD and CIDD subjects, respectively. These differences were statistically significant (p < 0.01 and p < 0.001, t-test). For each individual, site-specific recordings were made for plaque index and bleeding index scores, probing depth, loss of attachment and radiographic loss of alveolar bone. Dark field microscopy analysis of the presence of spirochetes, motile rods, cocci, non-motile rods, filaments and fusiforms was performed in the total of 106 pockets. According to the results of the dark field microscopy, the % of spirochetes and motile rods in the periodontally diseased pockets was significantly higher in the PIDD than in the CIDD subjects (9.2 +/- 13.4% and 10.8 +/- 14.3% versus 4.0 +/- 5.2% and 3.1 +/- 3.2%, p < 0.01 and p < 0.001, chi 2-test, respectively). Moreover, the PIDD subjects had lower mean %s of coccoid cells in periodontally diseased sites than the CIDD subjects (52.1 +/- 20.8% versus 60.7 +/- 9.0%, p < 0.001, chi 2-test).

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confidence: 99%

Dark field microscopy of the subgingival microflora in insulin‐dependent diabetics

Seppala

Ainamo

1996

J Clinic Periodontology

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“…Bacteriological study -Specimens: Were obtained gently and deep from the labial gingival crevice of both abutments (Könönen and Kumar 2015 (16) and Omar A.A. et al 1990) (25) using a sterile periodontal curette (Aas et al 2005) (25) .…”

Section: -Patients Were Not Smoker (Könönen and Kumar 2015)mentioning

confidence: 99%

Effect of Magnetic Attachment Flux on Sub-Gingival Pigmented Bacteroides Melaninogenicus Count in Magnetically Retained Overdenture Wearers

Amin

Bukhary

2018

Egyptian Dental Journal

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“…As técnicas de microscopia de contraste de fase e de campo escuro podem demonstrar diferenças de tamanho, forma e motilidade dos microrganismos presentes no biofilme dental. Porém, estes métodos microscópicos não diferenciam as espécies bacterianas (LOESCHE et al, 1985;BELTRAMI et al, 1987;OMAR et al, 1990;FURUICHI et al, 1996;DAHAN et al, 2004). O método de cultura, considerado o "padrão-ouro", é capaz de identificar diversos microrganismos presentes no biofilme dental, além de ser extremamente importante para a busca de novas espécies e para a determinação da susceptibilidade microbiana a diferentes antibióticos (NEWMAN e SOCRANSKY,1977;ALI et al, 1992;LIE et al, 1995 Hibridação DNA-DNA têm sido utilizada com sucesso na avaliação da composição da microbiota subgengival e de outros nichos da cavidade oral (HAFFAJEE et al, 1997a, b;FERES et al, 1999;CUGINI et al, 2000;XIMENEZ-FYVIE et al, 2000;FERES et al, 2001;FAVERI et al, 2006 (OLSEN et al, 1986;HUGENHOLTZ e PACE, 1996;HUGENHOLTZ et al, 1998;PASTER et al, 2001;KAZOR et al, 2003;KUMAR et al, 2005 …”

Section: Introductionunclassified

Diversidade bacteriana por análise clonal de 16S rRNA e pela hidridação DNA-DNA em amostras de biofilme subgengival de indivíduos portadores de periodontite agressiva.

Faveri¹

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Darkground microscopy of subgingival plaque from the top to the bottom of the periodontal pocket

Cited by 3 publications

References 29 publications

Dark field microscopy of the subgingival microflora in insulin‐dependent diabetics

Dark field microscopy of the subgingival microflora in insulin‐dependent diabetics

Effect of Magnetic Attachment Flux on Sub-Gingival Pigmented Bacteroides Melaninogenicus Count in Magnetically Retained Overdenture Wearers

Diversidade bacteriana por análise clonal de 16S rRNA e pela hidridação DNA-DNA em amostras de biofilme subgengival de indivíduos portadores de periodontite agressiva.

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