DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

Прошкина, Г. М.; Deyev, Sergey M.; Ryabova, A. V.; Tavanti, Francesco; Menziani, Maria Cristina; Cohen, Roy; Katrivas, Liat; Kotlyar, Alexander

doi:10.1021/acsami.9b10441

Cited by 17 publications

(20 citation statements)

References 23 publications

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“…In animal models, far-red fluorescent proteins, such as mCherry, can be applied to allow intravital visualization of a tumor [38]. Cherry and HER2-specific DARPin 9_29 were fused to obtain the recombinant protein DARPin-mCherry, which specifically stains HER2positive cancer cells [39] and is used for the functionalization of nanoparticles [40][41][42][43] as described below.…”

Section: Applications Of Darpins In Cancer Diagnosis and Therapymentioning

confidence: 99%

“…NaYF 4 : Yb 3 + Tm 3 + / NaYF 4 particles emitting ultraviolet radiation when exposed to infrared light were coated with the DARPin-mCherry protein [39], which allows visualization of cancer cells thanks to the far-red fluorescent mCherry module [40]. DARPin 9_29 and the DARPin-mCherry protein containing it were also used to coat 5-nm gold nanoparticles [41] and gold nanorods [42]. DARPin was efficiently coupled with the particle surface to form a crown consisting of approximately 35 protein molecules, thus reducing particle aggregation.…”

Section: Application Of Darpins In Nanoparticle Deliverymentioning

confidence: 99%

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DARPins: Promising Scaffolds for Theranostics

Shilova

Deyev

2019

Acta Naturae

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Monoclonal antibodies are the classical basis for targeted therapy, but the development of alternative binding proteins has made it possible to use non-immunoglobulin proteins as targeting modules. The advantages of DARPins, scaffold proteins based on ankyrin repeats, over antibodies are as follows: small size, stability over a wide range of temperatures and pH values, low aggregation tendency, and ease of production in heterologous expression systems. The differences in the structure of the paratope of DARPin and antibodies broaden the spectrum of target molecules, while the ease of creating hybrid fusion proteins allows one to obtain bispecific and multivalent constructs. In this article, we summarize recent data on the development of therapeutic and imaging compounds based on DARPins.

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Section: Applications Of Darpins In Cancer Diagnosis and Therapymentioning

confidence: 99%

Section: Application Of Darpins In Nanoparticle Deliverymentioning

confidence: 99%

DARPins: Promising Scaffolds for Theranostics

Shilova

Deyev

2019

Acta Naturae

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“…The IL-37 receptor complex is similar to the IL-18 receptor, another representative of the IL-1 family. IL-18 is one of the key pro-inflammatory cytokines acting as a pathogenetic factor in a number of diseases [41,42]. The IL-18 receptor complex consists of two chains: α (IL-18Rα) and β (IL-18Rβ), each of which has a TIR domain [43].…”

Section: Il-37 Receptor Complex and Signaling Pathwaysmentioning

confidence: 99%

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Shilovskiy¹,

Dyneva²,

Курбачева³

et al. 2019

Acta Naturae

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Cytokines of the interleukin-1 (IL-1) family play an important role in the realization of the protective functions of innate immunity and are the key mediators involved in the pathogenesis of a wide range of diseases, including various manifestations of allergy. The IL-1 family includes more than 11 members. However, the functions of many of them remain to be elucidated. Recently, new members of the IL-1 family have been discovered. In 2000, several independent research groups reported the discovery of a new interleukin of this family, which was named IL-37, or IL-1F7 (according to the new nomenclature). IL-37 was assigned to the IL-1 family based on its structural similarity with other members of this family. The study of its biological properties showed that its activity changes in inflammatory diseases, such as rheumatoid arthritis, psoriasis, as well as allergic diseases (allergic rhinitis, bronchial asthma, and atopic dermatitis). However, unlike most members of the IL-1 family, IL-37 acts as a negative regulator of inflammation. Activation of IL-37 suppresses inflammation, resulting in the suppression of inflammatory cytokines and chemokines, which in turn prevents infiltration of pro-inflammatory cells, mainly eosinophils and neutrophils. The exact molecular and cellular mechanisms of the anti-inflammatory effect of IL-37 in the development of allergic diseases (AD) have not been fully studied. This review summarizes and analyzes the accumulated experimental data on the role of IL-37 in the pathogenesis of AD, such as allergic rhinitis, bronchial asthma, and atopic dermatitis.

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“…In recent years, phototherapy has significantly advanced thanks to the use of lasers as light sources; nano-objects for the delivery of sensitizers [ 10 - 13 ]; targeted dyes [ 14 , 15 ]; increased dye circulation time in the blood [ 16 ]; and sustained release of dyes [ 17 ]. Also, conjugation of dyes with immunoadjuvants is promising in photoimmunotherapy because it leads to the triggering of a systemic immune response [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, nanotechnology has been actively used to develop photothermal sensitizing agents, such as gold nanoparticles [ 23 ], gold nanorods [ 10 ], upconverting nanoparticles [ 14 , 24 - 28 ], carbon nanotubes, graphene and its derivatives, and many others [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Near-infrared activated cyanine dyes as agents for photothermal therapy and diagnosis of tumors

et al. 2020

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Today, it has become apparent that innovative treatment methods, including those involving simultaneous diagnosis and therapy, are particularly in demand in modern cancer medicine. The development of nanomedicine offers new ways of increasing the therapeutic index and minimizing side effects. The development of photoactivatable dyes that are effectively absorbed in the first transparency window of biological tissues (700900 nm) and are capable of fluorescence and heat generation has led to the emergence of phototheranostics, an approach that combines the bioimaging of deep tumors and metastases and their photothermal treatment. The creation of near-infrared (NIR) light-activated agents for sensitive fluorescence bioimaging and phototherapy is a priority in phototheranostics, because the excitation of drugs and/or diagnostic substances in the near-infrared region exhibits advantages such as deep penetration into tissues and a weak baseline level of autofluorescence. In this review, we focus on NIR-excited dyes and discuss prospects for their application in photothermal therapy and the diagnosis of cancer. Particular attention is focused on the consideration of new multifunctional nanoplatforms for phototheranostics which allow one to achieve a synergistic effect in combinatorial photothermal, photodynamic, and/or chemotherapy, with simultaneous fluorescence, acoustic, and/or magnetic resonance imaging.

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DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

Cited by 17 publications

References 23 publications

DARPins: Promising Scaffolds for Theranostics

DARPins: Promising Scaffolds for Theranostics

The Role of Interleukin-37 in the Pathogenesis of Allergic Diseases

Near-infrared activated cyanine dyes as agents for photothermal therapy and diagnosis of tumors

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