Darwin’s “neuters” and the evolution of the sex continuum in a superorganism

Abstract

Ant castes are an amazing example of phenotypic plasticity. In worker-destined embryos of the antCardiocondyla obscurior, the default female developmental trajectory is interrupted even before the gonadal precursor cells acquire a sexual identity. miRNA and mRNA expression in embryos reveal three distinct phenotypic entities: males, females, and “neuters”, as Darwin coined the worker caste in “On the Origin of Species”. Based on these results we propose that haplodiploidy, in conjunction with insect sex determination, allows for the expression of a third dimension on the sex continuum, thus facilitating the evolution of individuals which develop traits their parents do not have.One sentence summarySex and caste differentiation begin during the same embryonic developmental window in the antCardiocondyla obscurior.