Das elektronenmikroskopische Bild der Epithelzellen aus embryonalen Hühnerlungen in der Gewebekultur

Merker, H. J.; Zimmermann, Bernd

doi:10.1007/bf02090945

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…These observations agree with the findings of Merker and Z immermann [22] and strongly support the suggestion of Ruefer et al [25] that Bisolvon may influence the metabolism of alveolar cells type II. An increased production might lead to an increased secretion of phospholi pids into the alveolar space, which could augment the amount of surfac tant available for spreading at the interface; nevertheless, our findings have only shown that the amount of osmophilic material in alveolar cells type II is increased.…”

Section: Ultrastructural Changes Of Lung Cells A) Alveolar Epithelialsupporting

confidence: 83%

“…The application of reliable morphometric methods has produced evi dence that there is more osmiophilic material in the alveolar cells type II after Bisolvon treatment; on the other hand, none of the authors [4,17,22,25], who have hitherto studied the surface activity of lung wash ings or the physiological or mechanical properties of the whole lungs of animals after administration of Bisolvon was able to demonstrate con clusive changes. Although it has never been unambiguously demonstrat ed, there is good evidence for the assumption that alveolar cells type II produce surface active material, so that these two statements seem to be contradictory.…”

Section: Theoretical Considerationsmentioning

confidence: 99%

“…Subsequent studies by means of the bubble-squeezing method did not lead to any conclusive results [4]; however, examination of lung washings with a Wilhelmy balance by R uefer et al [25] showed that Bisolvon treatment caused some alterations in the hysteresis loop; these authors suggested that Bisolvon could have an effect on the metabolism of alveolar cells; Merker and Z immermann [22] observed by means of tissue culture that pulmonary epithelial cells showed a tendency to develop towards type II alveolar cells under the influence of Bisolvon. It becomes evident that the observations reported by these authors were not conclusive, and partly even contradictory.…”

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confidence: 99%

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Morphometric Evaluation of Ultrastructural Changes in Type II Alveolar Cells of Rat Lung Produced by Bromhexine

Gil¹,

Thurnheer²

1971

Respiration

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The influence exerted by the secretolyticum Bisolvon® on rat lungs was examined. The lungs were fixed for electron microscopy by vascular perfusion of the fixatives in order to preserve the extracellular lining layer of alveoli (surfactant). The appearance of this lining layer was identical in Bisolvon-treated animals as compared with controls. But in Bisolvon-animals peculiar, unusually large forms of lamellated bodies of alveolar cells type II could be observed. A morphometric examination of this material demonstrated that the volume of lamellated bodies within alveolar cells type II, but especially the volume of alveolar epithelial cells type II, as compared with the total volume of lung tissue were clearly increased. The conclusion is drawn that these cells, supposed to represent the major source of alveolar surfactant, proliferate or differentiate under the influence of Bisolvon. The possible significance of this finding for the function of pulmonary surfactant is discussed. Additonally, concentric lamellar bodies, which appear to be proliferations of smooth endoplasmic reticulum, were found in alveolar cells type I.

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Section: Ultrastructural Changes Of Lung Cells A) Alveolar Epithelialsupporting

confidence: 83%

Section: Theoretical Considerationsmentioning

confidence: 99%

mentioning

confidence: 99%

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Morphometric Evaluation of Ultrastructural Changes in Type II Alveolar Cells of Rat Lung Produced by Bromhexine

Gil¹,

Thurnheer²

1971

Respiration

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“…Fell and co-workers, have been employed for this purpose as well as later methods with more sophisticated equipment [36,62,94,103,1851. Explanting lungs from day-1 1 mouse embryos in organ culture resulted in further branchings of the bronchial tract and in the development of lobules, but no further cellular differentiation occurred [7,45,117,133,150,1741. In general, the results of these early studies indicated that the more undifferentiated the organs are, the more satisfactory the differentiation obtained in vitro is.…”

Section: Introductionmentioning

confidence: 99%

Lung organoid culture

Zimmermann

1987

Differentiation

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“…More recently, Merker and Zimmermann [ 15] have shown in tissue culture that pulmonary epithelial cells show a tendency to develop towards pneumocytes of type II under the influ ence of bromexine. Their studies have led us to experimentally utilize bromexine be cause we wanted to discover if the admin istration of this drug in dogs in endotoxic shock can produce positive effects on the ultrastructural changes of the pulmonary parenchyma.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Changes of the Pulmonary Parenchyma after Experimentally Induced Endotoxic Shock in Dogs With and Without Drug Protection

Banna¹,

M²,

Murabito³

et al. 1985

Respiration

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The authors describe the ultrastructural alterations of the pulmonary parenchyma produced in dogs by endotoxic shock, and they examine the effects that a ‘secret-olytic’ drug (bromexine) has in modifying those changes. In the animals under shock there is a complete breakdown of the normal structure of the pulmonary parenchyma. According to the authors, these lesions are caused by the damage of the lining layer and of the cells which produce the constituents of the surfactant system. In dogs under shock and treated with bromexine the authors have seen a better organization of the pulmonary parenchyma: the cellular limits of the pneumocytes of types I and II were more clearly defined and the osmiophilic bodies were increased both in number and volume. The authors conclude that the damage of the lining layer and of the pneumocytes of type II plays an important role in the development of the ARDS and they say that bromexine can improve clinical and morphological aspects of that syndrome.

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Das elektronenmikroskopische Bild der Epithelzellen aus embryonalen Hühnerlungen in der Gewebekultur

Cited by 6 publications

References 17 publications

Morphometric Evaluation of Ultrastructural Changes in Type II Alveolar Cells of Rat Lung Produced by Bromhexine

Morphometric Evaluation of Ultrastructural Changes in Type II Alveolar Cells of Rat Lung Produced by Bromhexine

Lung organoid culture

Ultrastructural Changes of the Pulmonary Parenchyma after Experimentally Induced Endotoxic Shock in Dogs With and Without Drug Protection

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