Das Fettsäurespektrum einesCandida-Stammes nach Kultur aufn-Alkanen

Hornei, Sigrid; Köhler, Manfred; Weide, H.

doi:10.1002/jobm.3630120105

Cited by 29 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yeast cells were cultivated in a mineral salt medium (5 g NH 4 H 2 PO 4 , 2.5 g KH 2 PO 4 , 1 g MgSO 4 × 7 H 2 O, 0.02 g Ca(NO 3 ) 2 × 7 H 2 O, 2.0 mg FeCl 3 × 6 H 2 O, 0.5 mg H 3 BO 3 , 0.4 mg MnSO 4 × 5 H 2 O, 0.4 mg ZnSO 4 × 7 H 2 O, 0.2 mg Na 2 MoO 4 , 0.1 mg CuSO 4 × 5 H 2 O, 0.1 mg CoCl 2 , 0.1 mg KI in 1 L dest. H 2 O, pH 5.4 (Hornei et al [ 1972 ])) supplemented with 1% (v/v) vitamine solution (Van der Walt and van Kerken [ 1961 ]) and 1% (v/v) of the respective substrate (dodecane-2-one, dodec-1-ene, dodecane). Cultures grew until an OD 600nm of 3 at 30°C and 250 rpm for 31.5 h (dodecane), 32 h (dodec-1-ene), 40 h (dodecane-2-one, C. maltosa ), 290 h ( C. albicans ), 72 h ( C. catenulata ) 47 h ( C. tropicalis ), 189 h ( Y. lipolytica ) and 381 h ( T. asahii ).…”

Section: Methodsmentioning

confidence: 99%

Metabolism of alkenes and ketones by Candida maltosa and related yeasts

et al. 2014

View full text Add to dashboard Cite

Knowledge is scarce about the degradation of ketones in yeasts. For bacteria a subterminal degradation of alkanes to ketones and their further metabolization has been described which always involved Baeyer-Villiger monooxygenases (BVMOs). In addition, the question has to be clarified whether alkenes are converted to ketones, in particular for the oil degrading yeast Candida maltosa little is known. In this study we show the degradation of the aliphatic ketone dodecane-2-one by Candida maltosa and the related yeasts Candida tropicalis, Candida catenulata and Candida albicans as well as Trichosporon asahii and Yarrowia lipolytica. One pathway is initiated by the formation of decyl acetate, resulting from a Baeyer-Villiger-oxidation of this ketone. Beyond this, an initial reduction to dodecane-2-ol by a keto reductase was clearly shown. In addition, two different ways to metabolize dodec-1-ene were proposed. One involved the formation of dodecane-2-one and the other one a conversion leading to carboxylic and dicarboxylic acids. Furthermore the induction of ketone degrading enzymes by dodecane-2-one and dodec-1-ene was shown. Interestingly, with dodecane no subterminal degradation products were detected and it did not induce any enzymes to convert dodecane-2-one.

show abstract

Section: Methodsmentioning

confidence: 99%

Metabolism of alkenes and ketones by Candida maltosa and related yeasts

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Details on the mechanism of this conversion are not available. In general the occurrence of C16 and C 8 acids has been found (122,126,245) to be much lower for yeast grown on hydrocarbon (<C16) compared to glucose or acetate.…”

Section: Carbon Sourcementioning

confidence: 97%

“…The nature of the fatty acid component of cellular lipid reflects (Fig. 5) the composition of the alkane substrate (122,126,201,233,245,321,326) as well as the growth phase of the cells (201,321). Shorter-chain (<C13) n-alkanes are converted to various fatty acids apparently through the simultaneous operation of a chain elongation pathway and de novo synthesis after ,3-oxidation of the substrate.…”

Section: Carbon Sourcementioning

confidence: 99%

Lipids of yeasts.

Rattray¹,

Schibeci²,

Kidby³

1975

Bacteriological Reviews

191

View full text Add to dashboard Cite

“…Mineral salt medium for fungi (MSMHe) was used for cultivation, which consists of a basal medium [ 28 ] and a solution of trace elements [ 29 ]. Before autoclaving, the pH was adjusted to 5.4.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Mycoremediation of n-Alkanes and Branched-Chain Alkanes by Filamentous Fungi from Oil-Polluted Soil Samples in Kazakhstan

Gaid,

Pöpke,

Reinhard

et al. 2023

Microorganisms

View full text Add to dashboard Cite

For decades, researchers have focused on containing terrestrial oil pollution. The heterogeneity of soils, with immense microbial diversity, inspires them to transform pollutants and find cost-effective bioremediation methods. In this study, the mycoremediation potentials of five filamentous fungi isolated from polluted soils in Kazakhstan were investigated for their degradability of n-alkanes and branched-chain alkanes as sole carbon and energy sources. Dry weight estimation and gas chromatography–mass spectrometry (GC-MS) monitored the growth and the changes in the metabolic profile during degradation, respectively. Penicillium javanicum SBUG-M1741 and SBUG-M1742 oxidized medium-chain alkanes almost completely through mono- and di-terminal degradation. Pristane degradation by P. javanicum SBUG-M1741 was >95%, while its degradation with Purpureocillium lilacinum SBUG-M1751 was >90%. P. lilacinum SBUG-M1751 also exhibited the visible degradation potential of tetradecane and phytane, whereby in the transformation of phytane, both the mono- and di-terminal degradation pathways as well as α- and ß-oxidation steps could be described. Scedosporium boydii SBUG-M1749 used both mono- and di-terminal degradation pathways for n-alkanes, but with poor growth. Degradation of pristane by Fusarium oxysporum SBUG-M1747 followed the di-terminal oxidation mechanism, resulting in one dicarboxylic acid. These findings highlight the role of filamentous fungi in containing oil pollution and suggest possible degradation pathways.

show abstract

Das Fettsäurespektrum einesCandida-Stammes nach Kultur aufn-Alkanen

Cited by 29 publications

References 13 publications

Metabolism of alkenes and ketones by Candida maltosa and related yeasts

Metabolism of alkenes and ketones by Candida maltosa and related yeasts

Lipids of yeasts.

Characterization of the Mycoremediation of n-Alkanes and Branched-Chain Alkanes by Filamentous Fungi from Oil-Polluted Soil Samples in Kazakhstan

Contact Info

Product

Resources

About