To solve the problem of low efficiency of manual harvesting of green soybeans and lack of adaptable harvesters, in this study, a brushing-type green soybean harvester was designed. The comb-brushing type green soybean pod harvesting equipment is composed of a front-mounted separation drum, a full-width material delivery mechanism, a negative pressure cleaning system, and a stalk-pod separation system. Based on the operation requirements of the front-mounted brushing-type detachment drum, the drum parameters, parameters of comb arrangement, and structural parameters of the comb, the force analysis in detachment was performed. By taking the pod detachment rate and damage rate as the response indexes, the rotational speed of the drum, the travel speed of the device, and teeth distance as influencing factors, a three-factor five-level orthogonal rotary combination test was carried out by the software Design-Expert. By establishing mathematical regression models for various influencing factors and evaluation indicators, conducting variance analysis and significance analysis on the response indicators of each factor, the optimal parameters were obtained at a rotational speed of teeth of 397.36 rpm/min, minimum axial teeth distance of 4.8 mm and travel speed of the device of 0.5 m/s. Field test results showed that, under the optimal parameter combination, the pod detachment rate was 94%, the damage rate was 3.04%, the harvesting efficiency was greater than 0.187 hm2/h, and impurity content was less than 7.8%, all of which met the design and usage requirements. The research results can provide a reference for the design of soybean harvesters.