Context. Software is subject to the presence of faults, which impacts its quality as well as production and maintenance costs. Evolutionary fault localization uses data from the test activity (test spectra) as a source of information about defects, and its automation aims to obtain better accuracy and lower software repair cost. Motivation. Our analysis identified that the test spectra commonly used in research exhibit a high ratio of sample repetition, which impairs the training and evolution of Genetic Programming (GP) evolved heuristics. Problem. We investigate whether evolutionary training based on the uniqueness of suspiciousness scores can increase the ability to find software faults (defects), even in repeat-sample scenarios. Specifically, we examine whether the GP-evolved heuristic, which is based on the distinguishability of program elements in terms of faults, is really competitive. Methodology. The investigation formalized hypotheses, introduced three training strategies to guide the research and carried out an experimental evaluation, aiming to reach conclusions regarding the assessment of research questions and hypotheses. Analysis. The results have shown the competitiveness of all the proposed training strategies through evaluation metrics commonly used in the research field. Conclusion. Statistical analyses confirmed that the uniqueness of suspiciousness scores guides the generation of superior heuristics for fault localization.