Data in support of metabolic reprogramming in transformed mouse cortical astrocytes: A proteomic study

Abstract

2D-DIGE analysis coupled with mass spectrometry is a global, without a priori, comparative proteomic approach particularly suited to identify and quantify enzymes isoforms and structural proteins, thus making it an efficient tool for the characterization of the changes in cell phenotypes that occur in physiological and pathological conditions. In this data article in support of the research article entitled “Metabolic reprogramming in transformed mouse cortical astrocytes: a proteomic study” [1] we illustrate the changes in protein profile that occur during the metabolic reprogramming undergone by cultured mouse astrocytes in a model of in-vitro cancerous transformation [2].

Metabolic reprogramming in transformed mouse cortical astrocytes: A proteomic study

“…2) or of protein playing structural roles in the cytoskeleton (Fig. 3 in [30]) were observed. Those differential profiles are documented below.…”

“…and 1 is less abundant in TA (Fig. 7 in [30]). Likewise fructose-bis-P aldolase A (ALDO A) isoform was more abundant while its C isoform was less abundant.…”

“…4B-C, 5, 6 and Figs. 4-7 in [30]). In addition, proteomic analysis revealed a differential regulation of the isoforms of GAPDH, and fructose-bis-P aldolase.…”

“…1 in [30]. Proteins whose abundance is higher or lower in TA as compared to NA appear in red and green, respectively.…”

“…Among these spots, 117 spots were over-abundant and 160 were under-abundant in TA (Fig. 2 in [30]). Analysis by MALDI-TOF/TOF allowed the identification of 143 unique proteins from 235 of the 277 differentially expressed spots ( Table 2 and Table 1 in [30]).…”