Data management in Thrombin Generation

Hemker, H.C.; Kremers, Romy

doi:10.1016/j.thromres.2012.10.011

Cited by 105 publications

(119 citation statements)

References 29 publications

Supporting

Mentioning

118

Contrasting

Order By: Relevance

“…Thrombin generation determined in the CAT assay is a reproducible and reliable automated tool for assessment of overall thrombotic-haemostatic functions of plasma in an integrated manner [20][21][22]. This approach allowed us to document a prothrombotic state in persistent asthmatics, with the pattern of thrombin formation similar to that reported by Sneeboer et al [26] in asthma and Undas et al [27] in chronic obstructive pulmonary disease, which is an established risk factor for thromboembolic events [2].…”

Section: Thrombin Generationmentioning

confidence: 94%

“…Commercially available immunoenzymatic assays were used to measure PAI-1 antigen (American Diagnostica, Stamford, CT, USA) and platelet activation markers: PF4 and P-selectin (R&D Systems, Minneapolis, MN, USA). Measurement of plasma a 2 -macroglobulin [20], the Calibrated Automated Thrombogram (CAT) [21,22], computational assessment of thrombin generation kinetics [20], and Clot Lysis Time (CLT) [23] were determined using the previously described methods. A detailed methodology is provided in the online supplement.…”

Section: Laboratory Investigationsmentioning

confidence: 99%

See 1 more Smart Citation

Asthma is associated with enhanced thrombin formation and impaired fibrinolysis

Bazan-Socha

Mastalerz

Cybulska

et al. 2016

Clin Experimental Allergy

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Thrombin Generationmentioning

confidence: 94%

Section: Laboratory Investigationsmentioning

confidence: 99%

Asthma is associated with enhanced thrombin formation and impaired fibrinolysis

Bazan-Socha

Mastalerz

Cybulska

et al. 2016

Clin Experimental Allergy

Self Cite

View full text Add to dashboard Cite

show abstract

“…These two factors combine to cause a rapid decrease in the rate of change (d F /d t ) in the overall fluorescence after the initial “burst” of fluorescence. Using a mathematical calculation known as an H‐transform, it is possible to take the acquired data and to generate a diagnostic plot that accounts for the inner filter effect and the substrate consumption 14. However, even with this correction there can also be quenching from various compounds in the plasma that can change from sample to sample and can in turn affect the results of the plot 15.…”

Section: Substrates and Data Processingmentioning

confidence: 99%

A review of commercially available thrombin generation assays

Kintigh

Monagle

Ignjatović

2018

Research and Practice in Thrombosis and Haemostasis

View full text Add to dashboard Cite

Essentials Overview of the three commercial thrombin generation methods.Description of the sample preparation, data management, and analysis.Description of the similarities and differences in regards to substrates results.Discussion of advantages and disadvantages of the three approaches. Currently there are three commercially available thrombin generation methods. These methods help detect the levels of thrombin generated in patient samples by the use of chromogenic or fluorogenic substrates in plasma or whole blood. Determining the rate of thrombin generation can help indicate if patients are at risk of clotting or bleeding. This review discusses two fluorogenic and one chromogenic method and focuses on similarities and differences of these three methods. The review specifically focuses on the accuracy of commercial substrates used in thrombin generation, and interference that can occur by various plasma proteins, as well as on evaluating the advantages and disadvantages of each method. The commercial chromogenic assay and both fluorogenic assays are able to monitor the rate of thrombin generation and can give indications towards potential coagulation abnormalities. Overall, the main differences between the thrombin generation methods are based on the type of substrate used, sample preparation, and data processing. Despite advancement in this field there are still technical challenges that preclude the widespread use of thrombin generation in clinical applications.

show abstract

“…In all studies, thrombin generation potential (TGP) was measured in plateletpoor plasma (PPP) using the CAT method 36 as extensively described in Lavigne-Lissalde et al 18 Three biological TGP parameters were derived from the thrombogram analysis: the ETP (in nmol min 21 ) which corresponds to the area under the thrombogram curve, the peak thrombin generation (peak in nmol L 21 ) which represents the maximum amount of thrombin produced after induction by 5pM tissue factor (TF), and the lag time (LagT in minutes) which represents the time to the initial generation of thrombin after induction.…”

Section: Biological Measurementsmentioning

confidence: 99%

A meta-analysis of genome-wide association studies identifies ORM1 as a novel gene controlling thrombin generation potential

Rocañín-Arjó

Cohen

Carcaillon

et al. 2014

Blood

View full text Add to dashboard Cite

Key Points• Genetic variations at the ORM1 locus and concentrations of the encoded protein associate with thrombin generation.• These findings may guide the development of novel antithrombotic treatments.Thrombin, the major enzyme of the hemostatic system, is involved in biological processes associated with several human diseases. The capacity of a given individual to generate thrombin, called the thrombin generation potential (TGP), can be robustly measured in plasma and was shown to associate with thrombotic disorders. To investigate the genetic architecture underlying the interindividual TGP variability, we conducted a genome-wide association study in 2 discovery samples (N 5 1967) phenotyped for 3 TGP biomarkers, the endogenous thrombin potential, the peak height, and the lag time, and replicated the main findings in 2 independent studies (N 5 1254). We identified the ORM1 gene, coding for orosomucoid, as a novel locus associated with lag time variability, reflecting the initiation process of thrombin generation with a combined P value of P 5 7.1 3 10 215 for the lead single nucleotide polymorphism (SNP) (rs150611042). This SNP was also observed to associate with ORM1 expression in monocytes (P 5 8.7 3 10 210 ) and macrophages (P 5 3.2 3 10 23 ). In vitro functional experiments further demonstrated that supplementing normal plasma with increasing orosomucoid concentrations was associated with impaired thrombin generation. These results pave the way for novel mechanistic pathways and therapeutic perspectives in the etiology of thrombin-related disorders. (Blood. 2014;123(5):777-785)

show abstract

Data management in Thrombin Generation

Cited by 105 publications

References 29 publications

Asthma is associated with enhanced thrombin formation and impaired fibrinolysis

Asthma is associated with enhanced thrombin formation and impaired fibrinolysis

A review of commercially available thrombin generation assays

A meta-analysis of genome-wide association studies identifies ORM1 as a novel gene controlling thrombin generation potential

Contact Info

Product

Resources

About