Data on exopolysaccharides produced by Bacillus spp. from cassava pulp with antioxidant and antimicrobial properties

KARIRAT, Thipphiya; Saengha, Worachot; Deeseenthum, Sirirat; Ling, Nyuk; Sutthi, Nantaporn; Wang, Tiehui; Luang-In, Vijitra

doi:10.1016/j.dib.2023.109474

Cited by 4 publications

(5 citation statements)

References 8 publications

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“…However, according to some findings, growth independent EPS synthesis which continue even at the stationary phase when cell division and biomass is limited. In the presented findings, the similar accumulation of EPS was reported during early and mid-stationary phase which was due to extracellular release and activity of glycosidases whose function is independent of the viability and growth of cells [41].…”

Section: Determination Of Growth Pattern and Phase Of Eps Production ...supporting

confidence: 84%

“…Suitable optimization enhanced EPS synthesis in many bacillus strains viz. Bacillus licheniformis in optimized media produced the highest EPS yield of 48.57 g/L [41].…”

Section: Assessment Of the Efficacy Of Optimizationmentioning

confidence: 99%

“…Suitable optimization enhanced EPS synthesis in many bacillus strains viz. Bacillus licheniformis in optimized media produced the highest EPS yield of 48.57 g/L [41]. The effectiveness of the optimization procedure was evaluated by comparison of EPS production with unoptimized minimal media and culture conditions viz.…”

Section: Assessment Of the Efficacy Of Optimizationmentioning

confidence: 99%

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Development of Biological Coating from Novel Halophilic Exopolysaccharide Exerting Shelf-Life-Prolonging and Biocontrol Actions for Post-Harvest Applications

Upadhyaya,

Patel,

Patel

et al. 2024

Molecules

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The literature presents the preserving effect of biological coatings developed from various microbial sources. However, the presented work exhibits its uniqueness in the utilization of halophilic exopolysaccharides as food coating material. Moreover, such extremophilic exopolysaccharides are more stable and economical production is possible. Consequently, the aim of the presented research was to develop a coating material from marine exopolysaccharide (EPS). The significant EPS producers having antagonistic attributes against selected phytopathogens were screened from different marine water and soil samples. TSIS01 isolate revealed the maximum antagonism well and EPS production was selected further and characterized as Bacillus tequilensis MS01 by 16S rRNA analysis. EPS production was optimized and deproteinized EPS was assessed for biophysical properties. High performance thin layer chromatography (HPTLC) analysis revealed that EPS was a heteropolymer of glucose, galactose, mannose, and glucuronic acid. Fourier transform infrared spectroscopy, X-ray diffraction, and UV-visible spectra validated the presence of determined sugars. It showed high stability at a wide range of temperatures, pH and incubation time, ≈1.63 × 106 Da molecular weight, intermediate solubility index (48.2 ± 3.12%), low water holding capacity (12.4 ± 1.93%), and pseudoplastic rheologic shear-thinning comparable to xanthan gum. It revealed antimicrobial potential against human pathogens and antioxidants as well as anti-inflammatory potential. The biocontrol assay of EPS against phytopathogens revealed the highest activity against Alternaria solani. The EPS-coated and control tomato fruits were treated with A. solani suspension to check the % disease incidence, which revealed a significant (p < 0.001) decline compared to uncoated controls. Moreover, it revealed shelf-life prolonging action on tomatoes comparable to xanthan gum and higher than chitosan. Consequently, the presented marine EPS was elucidated as a potent coating material to mitigate post-harvest losses.

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Section: Determination Of Growth Pattern and Phase Of Eps Production ...supporting

confidence: 84%

“…Suitable optimization enhanced EPS synthesis in many bacillus strains viz. Bacillus licheniformis in optimized media produced the highest EPS yield of 48.57 g/L [41].…”

Section: Assessment Of the Efficacy Of Optimizationmentioning

confidence: 99%

Section: Assessment Of the Efficacy Of Optimizationmentioning

confidence: 99%

See 1 more Smart Citation

Development of Biological Coating from Novel Halophilic Exopolysaccharide Exerting Shelf-Life-Prolonging and Biocontrol Actions for Post-Harvest Applications

Upadhyaya,

Patel,

Patel

et al. 2024

Molecules

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Section: Methodsmentioning

confidence: 99%

“…OP522324.1) and Escherichia coli TISTR 527 (ATCC 11775) (GenBank accession no. X80725.1) were obtained from Thailand Institute of Scientific and Technological Research (TISTR), while Streptococcus agalactiae EW1 was sourced from diseased Nile tilapia in the northeastern region [27]. Luria-Bertani (LB) broth (HiMedia, Maharashtra, India) was used to grow all bacteria to 10 8 CFU/mL after 24 h at 37 • C. Each 4 mm paper disc was pipetted with 20 µL of sterilized extract (20 mg/mL), placed on LB agar plates inoculated with 100 µL of bacterial suspension, and cultivated at 37 • C for 48 h. The inhibitory zone (mm diameter) as antimicrobial activity was measured in triplicate.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Exploring the Bioactive Potential of Calostoma insigne, an Endangered Culinary Puffball Mushroom, from Northeastern Thailand

Saengha,

Karirat,

Pitisin

et al. 2023

Foods

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Calostoma insigne puffball mushrooms are only found in forests with rich biodiversity in very few countries including Thailand, and their biofunctions remain largely unexplored. This study used the agar disk diffusion assay, the anti-glucosidase assay, and the 3, 4, 5-dimethylthiazol-2-yl-2-5-diphenyltetrazolium bromide (MTT) assay to evaluate the bioactive potential of these endangered puffball mushrooms. Internal transcribed spacer (ITS) gene analysis identified C. insigne, a puffball mushroom with green, globose, and spiny spores. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the polysaccharide structure while scanning electron microscopy (SEM) revealed a fiber-like network. The ethanolic gelatinous fruiting body extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging capacity (57.96%), a ferric ion-reducing antioxidant power (FRAP) value of 1.73 mg FeSO4/g, and α-glucosidase inhibition (73.18%). C. insigne cytotoxicity was effective towards HT-29 colon cancer cells using the MTT assay (IC50 of 770.6 µg/mL at 72 h) and also showed antiproliferative capacity (IC50 of 297.1 µg/mL). This puffball mushroom stimulated apoptotic genes and proteins (caspase-3, Bax, and p21) via an intrinsic apoptotic pathway in HT-29 cells. In the laboratory, the medium formula consisting of 20% potato, 2% sucrose, and 0.2% peptone was optimal to increase fungal mycelial biomass (2.74 g DW/100 mL), with propagation at pH 5.0 and 30 °C. Puffball mushrooms are consumed as local foods and also confer several potential health benefits, making them worthy of conservation for sustainable utilization.

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New insights into the effect of xylooligosaccharide derived from agricultural waste, single or combined dietary supplementation with mixed probiotics on growth, flesh quality, health condition and disease resistance in Nile tilapia (Oreochromis niloticus)

Wangkahart,

Nontasan,

Phudkliang

et al. 2024

Carbohydrate Polymer Technologies and Applications

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Data on exopolysaccharides produced by Bacillus spp. from cassava pulp with antioxidant and antimicrobial properties

Cited by 4 publications

References 8 publications

Development of Biological Coating from Novel Halophilic Exopolysaccharide Exerting Shelf-Life-Prolonging and Biocontrol Actions for Post-Harvest Applications

Development of Biological Coating from Novel Halophilic Exopolysaccharide Exerting Shelf-Life-Prolonging and Biocontrol Actions for Post-Harvest Applications

Exploring the Bioactive Potential of Calostoma insigne, an Endangered Culinary Puffball Mushroom, from Northeastern Thailand

New insights into the effect of xylooligosaccharide derived from agricultural waste, single or combined dietary supplementation with mixed probiotics on growth, flesh quality, health condition and disease resistance in Nile tilapia (Oreochromis niloticus)

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