Data on Manuka Honey/Gellan Gum composite hydrogels for cartilage repair

Abstract

This work contains original data supporting our research paper “Antibacterial effectiveness meets improved mechanical properties: Manuka Honey/Gellan Gum composite hydrogels for cartilage repair”, Bonifacio et al., in press [1], in which innovative composite hydrogels, based on Gellan Gum/Manuka honey/Halloysite nanotubes were described as biomaterials for cartilage regeneration. Here the composites were further examined by means of Fourier Transform Infrared Spectroscopy, in Attenuated Total Reflectance mode (FT-IR/ATR). Materials devoted to cartilage replacement must possess adequate fluid permeability and lubricating capability, therefore, a deeper investigation on water uptake kinetics of freeze-dried specimens up to 21 days in PBS was carried out. Moreover, since the degradation rate of a biomaterial plays a pivotal role in tissue engineering, weight loss measurements of the prepared hydrogels were performed in simulated synovial fluid, in phosphate buffer solution (PBS) and in lysozyme. Scanning Electron Microscopy images provide insight into the morphology of the freeze-dried samples.Finally, additional information on Staphylococcus aureus and Staphylococcus epidermidis ability to adhere onto the prepared hydrogel composites in short times were obtained, as well as the chondrogenic potential of the composites assessed by SDS-PAGE followed by Coomassie blue gel staining.

Investigating Manuka Honey Antibacterial Properties When Incorporated into Cryogel, Hydrogel, and Electrospun Tissue Engineering Scaffolds

“…El-Malek et al [31] incorporated 20% (v/v) MH into a chitosan-gelatin hydrogel, resulting in antibacterial properties to S. aureus , Streptococcus pyogenes , Acinetobacter baumannii , Pseudomonas aeruginosa , and Proteus mirabilis . Similarly, two studies completed by Bonifacio et al [32,33] examined a MH/gellan gum composite hydrogel which led to the inhibition of both S. aureus and S. epidermidis clinical isolates. As this was targeting cartilage repair, mesenchymal stem cells were also seeded on the scaffolds and, in addition to no cytotoxic effect, collagen II expression and synthesis of glycosaminoglycans (GAGs) and proteoglycans was observed.…”

Applications of Beehive Products for Wound Repair and Skin Care

“…Furthermore, scaffolds represent an important help for wound and tissue repair [110], and honey can be very useful for scaffold synthesis [111]. Scaffolds created with manuka honey and gellan gum are able to promote cartilage healing, stimulating the growth and differentiation of chondroblasts [112,113].…”

Antibacterial Efficacy of Manuka Honey-Doped Chitosan-Gelatin Cryogel and Hydrogel Scaffolds in Reducing Infection

“…To initially determine the best method to incorporate MH into the 4%-chitosan-gelatin scaffolds, MH was added to the scaffolds at different stages using four mixing methods. Note that the concentration of MH used (0, 1, 5, or 10%) was based on previous work by our group and others, demonstrating the advantages of these different concentrations related to bacterial clearance and scaffold properties [17,19,20,37].…”

“…This study tested scaffolds against E. coli, P. aeruginosa, S. aureus, Bacillus subtilis, and the fungus Candida albicans, concluding that chitosan-lactic acid combined with 6% MH was effective against S. aureus and E. coli. Similarly, Bonifacio et al [19] combined 2% MH with six types of gellangum-based hydrogels, which were found to be effective against S. aureus and Staphylococcus epidermidis. Our group also incorporated MH into silk-based cryogels and hydrogels at 1, 5, and 10% concentrations and evaluated these scaffolds against biofilm formation by S. aureus [15].…”