“…4 PCR was well-described as: "The process which comprises treating of separate complementary strands of a target nucleic acid with a molar excess of two primers and extending the primers to form complementary primer extension products which in turn act as templates for synthesizing the desired nucleic acid sequence". 5 PCR technique has been used immensely for a wide variety of applications including mutation detection, 6 gene cloning, 7 genotyping, 8 microarray, 9 DNA sequencing, 10 ngerprinting, 11 paternity testing, 12 pathogen detection, 13 forensics 14 and diagnostics. 15 The signicance of PCR originates from its ability to amplify trace amounts of DNA or cDNA (complementary DNA) sequences within minutes in a reaction realized in an automated machine.…”