Databases, quality control and interpretation of DNA profiling in the Home Office Forensic Science Service

Gill, Peter; Evett, I.W.; Woodroffe, Susan; Lygo, Joan E.; Millican, Emma S.; Webster, Mark

doi:10.1002/elps.1150120217

Cited by 38 publications

(8 citation statements)

References 7 publications

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“…There are also differences in measurement error between laboratories [25,26]. These deficiencies are accounted for by use of marker DNA fragments of known nucleotide base pair length to assist in estimating cleaved DNA fragment sizes; however, acknowledgement of remaining error of measurement of the size of detectable fragments is inherent in the application of a tolerance range for considering fragments of similar but different sizes as a "match" [27]. Laboratory error is also inherent in partial genome sequencing [28].…”

Section: Discussionmentioning

confidence: 99%

A computer simulation analysis of the accuracy of partial genome sequencing and restriction fragment analysis in estimating genetic relationships: an application to papillomavirus DNA sequences

Qiao

Weigel

2004

BMC Bioinformatics

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Background: Determination of genetic relatedness among microorganisms provides information necessary for making inferences regarding phylogeny. However, there is little information available on how well the genetic relationships inferred from different genotyping methods agree with true genetic relationships. In this report, two genotyping methods -restriction fragment analysis (RFA) and partial genome DNA sequencing -were each compared to complete DNA sequencing as the definitive standard for classification.

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Section: Discussionmentioning

confidence: 99%

A computer simulation analysis of the accuracy of partial genome sequencing and restriction fragment analysis in estimating genetic relationships: an application to papillomavirus DNA sequences

Qiao

Weigel

2004

BMC Bioinformatics

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“…Within any locus, there is little variation in c (except for D4S139). From casework data the value of c for YNH24 has been estimated [9] (see also [8]) to be 0.008. This value seems to agree with the average value of c estimated from data on the sample of bloodstains, excluding the data of D4S139.…”

Section: "Matching": the Current Procedures And Some Problemsmentioning

confidence: 99%

DNA fingerprinting for forensic identification: Some statistical issues

Majumder

1995

Electrophoresis

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In DNA fingerprinting work, it is well known that, although fragment lengths of a sample are uncorrelated, measurement errors of fragment lengths are correlated. Unless the fragment length is very large, the measurement errors are directly proportional to fragment lengths. In the currently practiced matching procedures for forensic identification, these facts are ignored or inadequately used. Some of these issues are addressed in this study. Based on available empirical observations, an appropriate statistical model and techniques are proposed. These techniques lead to the definition and construction of elliptical match windows, instead of independent interval (linear) windows currently used. Simulation experiments indicate that the performance of the proposed procedure is superior to the current procedures. Further, the proposed procedure does not make use of some assumptions underlying the current procedures. Extensions of the procedure to utilize replicate measurements of crime-scene and/or suspect's samples are indicated.

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“…They performed withinperson comparisons on the duplicate results and betweenperson comparisons on the results from different individuals. They concluded that the method described by Gill et al [5] was sufficiently robust for use in forensic casework.…”

Section: Introductionmentioning

confidence: 97%

“…Gill et al [5] described the basic match/binning methodology used by the FSS. This involves a 2.8% match guideline and a corresponding 5.6% sliding window to provide a conservative estimate of database frequencies.…”

Section: Introductionmentioning

confidence: 99%

Large scale database experiments to assess the significance of matching DNA profiles

1995

Self Cite

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Over 5,700 three-probe VNTR DNA profiles collected by several United Kingdom (UK) laboratories have been compared to examine the probability of randomly matching 2 samples from different individuals. In over 16 million comparisons, using a matching rule corresponding to the matching guideline employed by the UK Forensic Science Service, no profiles were found to match at the 3 loci D1S7 (MS1), D7S21(MS31) and D12S11 (MS43a). The frequency of occurrence of a set of Caucasian profiles have been estimated with 6 reference databases. The results show that there were greater differences in the frequency estimates when using a database of Afro-Caribbean or Asian profiles, rather than a different Caucasian database. The results further demonstrate the power and robustness of the VNTR DNA profiling technique for forensic casework.

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Databases, quality control and interpretation of DNA profiling in the Home Office Forensic Science Service

Cited by 38 publications

References 7 publications

A computer simulation analysis of the accuracy of partial genome sequencing and restriction fragment analysis in estimating genetic relationships: an application to papillomavirus DNA sequences

A computer simulation analysis of the accuracy of partial genome sequencing and restriction fragment analysis in estimating genetic relationships: an application to papillomavirus DNA sequences

DNA fingerprinting for forensic identification: Some statistical issues

Large scale database experiments to assess the significance of matching DNA profiles

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