Dataset of 16S ribosomal DNA sequences of bacteria isolated from marine red algae Kappaphycus alvarezii

Abstract

The data provided in the article contains bacterial community profiles present on the surface of red algae ( Kappaphycus alvarezii ) isolated directly after collection and after 30 days of cultivation in a closed circulation system. The explants of Kappaphycus alvarezii were cultivated in a laboratory setting under controlled growth conditions for 30 days in order to determine bacteria that could adapt to controlled culture conditions. Amplification and sequencing of bacterial 16S rDNA amplicon were performed on bacterial isolates associated with the seedlings. The 16S rDNA gene sequences were analyzed, trimmed, and assembled into contigs using DNA Baser Sequence Assembler (V5) software. Taxonomic identification for the assembled sequences was achieved using the online BLAST (blastn) algorithm, and the construction of a phylogenetic tree was performed using the MEGA7 software. The data reveals a distinct set of microbial variations between day one and day 30. The phylogenetic tree depicts four major clusters, Vibrio, Pseudoalteromonas, Alteromonas , and Bacterioplanes resident on the surface of the K. alvarezii . Comparison between these two bacterial groups provides evidence of the persistent marine bacteria that adapt to the long-term culture in closed circulation systems. Raw data files are available at the GenBank, NCBI database under the accession number of MZ570560 to MZ570580.

Dataset of 16S ribosomal DNA sequence-based identification of endophytic bacteria isolated from healthy and diseased Sabah red algae, Kappaphycus alvarezii

“…On days 2, 5, 10, and 15, colonies were selected and purified using liquid broth. Endophytic bacterial colonies were chosen for each petri dish under consideration based on their stage of growth and morphology [2] .…”

Genomic analysis of endophytic Bacillus altitudinis strain VUMS1 from Kappaphycus alvarezii and its inhibitory effect against Vibrio parahaemolyticus

“…Wizard Genomic DNA Purification Kit (Promega, USA) was used to extract the genomic DNA (gDNA). DNA extraction was performed based on the manufacturer's protocol (Rupert et al, 2022). The extracted gDNA was sent to NeoScience Sdn.…”

“…Endophytic bacteria are good candidates for promoting the growth and overall health of their host plants. In recent studies, endophytic bacteria are used as a biocontrol agent against harmful pathogens (Senthilkumar et al, 2011;Morales-Cedeño et al, 2021;An et al, 2022). Endophytic bacteria are microorganisms that live within the tissues of plants without causing any apparent harm to the host.…”

“…Endophytes have evolved to live inside the host plant's endosphere, which refers to the internal tissues and spaces within the plant, to create a specialised niche that offers them protection from the external environment. Since pathogenic bacteria and endophytic bacteria inhabit the same niche, endophytic bacteria are the best candidate to biologically control plant pathogens (Senthilkumar et al, 2011). Bacillus altitudinis has been found in a variety of settings, including the southern Indian Ocean, deep freshwater in Manasbal Lake, soil and silt (Kumar et al, 2010;Mao et al, 2013;Halder et al, 2017;Shafi et al, 2017).…”

Antibacterial potential of indigenous medicinal plants against methicillin-resistant Staphylococcus aureus isolated from septic wounds

“…Biochemical testing (catalase test, blood hemolysis and coagulase test) was carried out according to Bergey's manual of determinative bacteriology's protocol (Holt et al, 1994). Coagulasepositive isolates were selected and preserved in glycerol (15%) for further reference (Rupert et al, 2022).…”