Dataset on a 173 region awake resting state quantitative cerebral blood volume rat brain atlas and regional changes to cerebral blood volume under isoflurane anesthetization and CO2 challenge

Gharagouzloo, Codi; Timms, Liam; Qiao, Junfei; Fang, Zihang; Nneji, Joseph; Pandya, Aniket; Kulkarni, Praveen; Ven, Anne L. van de; Ferris, Craig F.; Sridhar, Srinivas

doi:10.1016/j.dib.2018.01.021

Cited by 3 publications

(15 citation statements)

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“…The mean signal intensity was used to calculate the qCBV for each brain area. Dataset 1 of (Gharagouzloo et al, 2017) lists all brain areas and the average percent volume of qCBV for each. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Given that a Gaussian distribution implies homogeneity, combined with the observation that all Type I regions had a qCBV maximum of ~10% (Dataset 1 (Gharagouzloo et al, 2017)), suggests that Type I regions are primarily filled with capillaries of uniform size and density. For example, the somatosensory cortices (region #s 130–137), rostral piriform (#103), lateral orbital (#75), ventral orbital (#166), parietal (#116), prelimbic (#115) and secondary motor (#80) sections of the cortex all provided highly Gaussian intensity distributions with

R_{Gauss}^{2} > 0.9

.…”

Section: Resultsmentioning

confidence: 99%

“…The overall distribution of regional qCBV values is captured in Fig. 2d and each value is listed explicitly in Dataset 1 in (Gharagouzloo et al, 2017). There are 44 areas with qCBV of 10% or higher (means highlighted in yellow).…”

Section: Resultsmentioning

confidence: 99%

“…Deviations were generally highest in regions including or bordering large vessels and visual inspection revealed noticeable variation in path and morphology of these vessels in the angiographic images. The resting state qCBV values for all animals in all regions are provided in Dataset 1 of our Data In Brief article (Gharagouzloo et al, 2017). The inter-animal variation was actually lowest on average for the isoflurane state relative to the other states which suggests that, while the dose was adjusted throughout the scan time, there was major dose dependence for the isoflurane induced state.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, since we are analyzing a large number of brain regions (173), the positive false discovery rate (pFDR) was also calculated and is included alongside the p-values for reference (Storey, 2002). Full lists of all changes for both states are presented in Datasets 2&3 of the Data in Brief (Gharagouzloo et al, 2017). …”

Section: Methodsmentioning

confidence: 99%

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Quantitative vascular neuroimaging of the rat brain using superparamagnetic nanoparticles: New insights on vascular organization and brain function

et al. 2017

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A method called Quantitative Ultra-Short Time-to-Echo Contrast Enhanced (QUTE-CE) Magnetic Resonance Imaging (MRI) which utilizes superparamagnetic iron oxide nanoparticles (SPI-ONs) as a contrast agent to yield positive contrast angiograms with high clarity and definition is applied to the whole live rat brain. QUTE-CE MRI intensity data are particularly well suited for measuring quantitative cerebral blood volume (qCBV). A global map of qCBV in the awake resting-state with unprecedented detail was created via application of a 3D MRI rat brain atlas with 173 segmented and annotated brain areas. From this map we identified two distributed, integrated neural circuits showing the highest capillary densities in the brain. One is the neural circuitry involved with the primary senses of smell, hearing and vision and the other is the neural circuitry of memory. Under isoflurane anesthesia, these same circuits showed significant decreases in qCBV suggesting a role in consciousness. Neural circuits in the brainstem associated with the reticular activating system and the maintenance of respiration, body temperature and cardiovascular function showed an increase in qCBV with anesthesia. During awake CO2 challenge, 84 regions showed significant increases relative to an awake baseline state. This CO2 response provides a measure of cerebralvascular reactivity and regional perfusion reserve with the highest response measured in the somatosensory cortex. These results demonstrate the utility of QUTE-CE MRI for qCBV analysis and offer a new perspective on brain function and vascular organization.

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Section: Resultsmentioning

confidence: 99%

R_{Gauss}^{2} > 0.9

.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Quantitative vascular neuroimaging of the rat brain using superparamagnetic nanoparticles: New insights on vascular organization and brain function

et al. 2017

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Neurovascular imaging with QUTE-CE MRI in APOE4 rats reveals early vascular abnormalities

Leaston¹,

Ferris

Kulkarni

et al. 2021

PLoS ONE

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Cerebrovascular abnormality is linked to Alzheimer’s disease and related dementias (ADRDs). ApoE-Ɛ4 (APOE4) is known to play a critical role in neurovascular dysfunction, however current medical imaging technologies are limited in quantification. This cross-sectional study tested the feasibility of a recently established imaging modality, quantitative ultra-short time-to-echo contrast-enhanced magnetic resonance imaging (QUTE-CE MRI), to identify small vessel abnormality early in development of human APOE4 knock-in female rat (TGRA8960) animal model. At 8 months, 48.3% of the brain volume was found to have significant signal increase (75/173 anatomically segmented regions; q<0.05 for multiple comparisons). Notably, vascular abnormality was detected in the tri-synaptic circuit, cerebellum, and amygdala, all of which are known to functionally decline throughout AD pathology and have implications in learning and memory. The detected abnormality quantified with QUTE-CE MRI is likely a result of hyper-vascularization, but may also be partly, or wholly, due to contributions from blood-brain-barrier leakage. Further exploration with histological validation is warranted to verify the pathological cause. Regardless, these results indicate that QUTE-CE MRI can detect neurovascular dysfunction with high sensitivity with APOE4 and may be helpful to provide new insights into health and disease.

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Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts

Leaston¹,

Qiao

Harding

et al. 2021

Front. Neurol.

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This was an exploratory study designed to evaluate the feasibility of a recently established imaging modality, quantitative ultrashort time-to-echo contrast enhanced (QUTE-CE), to follow the early pathology and vulnerability of the blood brain barrier in response to single and repetitive mild head impacts. A closed-head, momentum exchange model was used to produce three consecutive mild head impacts aimed at the forebrain separated by 24 h each. Animals were measured at baseline and within 1 h of impact. Anatomical images were collected to assess the extent of structural damage. QUTE-CE biomarkers for BBB permeability were calculated on 420,000 voxels in the brain and were registered to a bilateral 3D brain atlas providing site-specific information on 118 anatomical regions. Blood brain barrier permeability was confirmed by extravasation of labeled dextran. All head impacts occurred in the absence of any structural brain damage. A single mild head impact had measurable effects on blood brain barrier permeability and was more significant after the second and third impacts. Affected regions included the prefrontal ctx, basal ganglia, hippocampus, amygdala, and brainstem. Our findings support the concerns raised by the healthcare community regarding mild head injuries in participants in organized contact sports and military personnel in basic training and combat.

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Dataset on a 173 region awake resting state quantitative cerebral blood volume rat brain atlas and regional changes to cerebral blood volume under isoflurane anesthetization and CO2 challenge

Cited by 3 publications

References 3 publications

Quantitative vascular neuroimaging of the rat brain using superparamagnetic nanoparticles: New insights on vascular organization and brain function

Quantitative vascular neuroimaging of the rat brain using superparamagnetic nanoparticles: New insights on vascular organization and brain function

Neurovascular imaging with QUTE-CE MRI in APOE4 rats reveals early vascular abnormalities

Quantitative Imaging of Blood-Brain Barrier Permeability Following Repetitive Mild Head Impacts

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