Datasets of microarray analysis to identify Gpr137b-dependent interleukin-4-responsive genes in the mouse macrophage cell line RAW264

Abstract

Macrophages are classified mainly into two subtypes, M1 and M2, which exhibit distinct phenotypes, based on their microenvironment. We have recently demonstrated that Gpr137b is abundantly expressed in RAW264 macrophages, “ Gpr137b is an orphan G-protein-coupled receptor associated with M2 macrophage polarization” (Islam et al., in press) [1]. Although recent studies have suggested that G-protein-coupled receptors (GPCRs) are associated with M1/M2 macrophage polarization (“G-protein-coupled bile acid receptor 1 (GPBAR1, TGR5) agonists reduce the production of proinflammatory cytokines and stabilize the alternative macrophage phenotype” (Hogenauer et al., 2014) [2] , “Leukotriene B4 promotes neovascularization and macrophage recruitment in murine wet-type AMD models” (Sasaki et al., 2018) [3] ), available information about GPCR-mediated macrophage polarization is still limited. This prompted us to generate Gpr137b -knockout (KO) RAW264 clones using the CRISPR/Cas9 genome editing system to elucidate the function of Gpr137b in interleukin (IL)-4-induced M2 macrophage polarization (Islam et al., in press) [1]. Here we present the datasets of a microarray analysis to identify Gpr137b -dependent IL-4-responsive genes in RAW264 cells. The raw microarray data are available in the Gene Expression Omnibus database ( https://www.ncbi.nlm.nih.gov/geo/ ) under the accession number GSE117578, https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117578 .

Intestinal Inflammation Alters the Expression of Hepatic Bile Acid Receptors Causing Liver Impairment

“…Recent data reported that TGR5 is highly expressed in monocytes/macrophages (36,38,39), members of the mononuclear phagocyte system that circulate through the blood and extravasate into inflamed tissues. We performed immunohistochemistry and confirmed that TGR5 is principally localized in the macrophage infiltrate of murine colonic samples, while FXR is mainly found in the nuclei of enterocytes (in uninflamed tissue).…”

“…Recent data reported that TGR5 is highly expressed in monocytes/macrophages (35,37,38), members of the mononuclear phagocyte system that circulate through the blood and extravasate à P < 0.05; Ãà P < 0.01. FXR ¼ farnesoid-X-receptor; PXR ¼ pregnane-X-receptor; TEER ¼ transepithelial electric resistance.…”