Dating the Endometrial Biopsy

Noyes, R. W.; Hertig, Arthur T.; Rock, John

doi:10.1016/s0015-0282(16)30062-0

Cited by 2,824 publications

(662 citation statements)

References 11 publications

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“…None of the patients had received hormone therapy. The normal endometria had been 'dated' on hematoxylin-and eosin-stained sections by using the histological criteria of Noyes et al 12 All cancer patients had been treated surgically with total abdominal hysterectomy and bilateral salpingo-oophorectomy. No lymph node sampling of the iliac nodes was performed.…”

Section: Methodsmentioning

confidence: 99%

Loss of expression and nuclear/cytoplasmic localization of the FOXP1 forkhead transcription factor are common events in early endometrial cancer: relationship with estrogen receptors and HIF-1α expression

et al. 2006

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The FOXP1 gene has been identified as a new member of the winged helix family of transcription factors that have important roles in cellular transformation, differentiation and proliferation. In this study, we examined the expression of FOXP1 in the normal and malignant endometrium (stage I endometrioid adenocarcinoma cases), showing a frequent deregulation of its expression in cancer. Proliferative endometrium showed predominantly nuclear localization of FOXP1, while exclusively weak cytoplasmic staining was present in the secretory phase. Loss of nuclear expression was the most striking event in endometrial adenocarcinoma. Nuclear expression ranged from 0 to 20% (median 0%). Cytoplasmic expression was noted more frequently, ranging from 0 to 90% of cancer cells (median 30%). Overall, 24/82 cases (29.3%) were observed to lack both nuclear and cytoplasmic FOXP1 expression. Tumors with exclusively cytoplasmic expression of FOXP1 were linked with deep myometrial invasion and hypoxia-inducible factors 1a (HIF-1a) expression. On the other hand, the presence of nuclear FOXP1 expression was significantly linked with ER-a reactivity. Survival analysis did not reveal significant differences among patients grouped by FOXP1 expression, presumably due to the high curability of stage I disease. This study provides evidence on pathways to be investigated to elucidate the interplay between FOXP1, ER-a and HIF-1a in hormone dependent cancers.

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Section: Methodsmentioning

confidence: 99%

Loss of expression and nuclear/cytoplasmic localization of the FOXP1 forkhead transcription factor are common events in early endometrial cancer: relationship with estrogen receptors and HIF-1α expression

et al. 2006

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“…The specimens were fixed overnight in 4% paraformaldehyde (in phosphate buffer (pH 7.3)), embedded in paraffin, and cut into 4 mm sections. After being dewaxed in xylene and rehydrated in a graded series of ethanol, the sections were dated according to the standard criteria 25 and immunostained with 4H84 to exclude the presence of the invasive trophoblasts. The remaining sections were used for immunohistochemistrical analysis.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis

2010

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Chronic endometritis is often identified in the patients with unexplained infertility, and is histopathologically characterized by infiltration of plasmacytes within the endometrial stroma. In parallel with stromal plasmacyte infiltration, the endometrial functional layer in chronic endometritis is invaded by B cells, which are a rare leukocyte subset residing within the basal layer in the nonpathological endometrium. In this study, we investigated the molecular expression underlying this unusual increase of B cells in chronic endometritis. Twenty-two out of 76 infertile patients were diagnosed with chronic endometritis from the stromal plasmacyte infiltration, and the endometrium contained numerous stromal B-cell aggregates and glandular single B cells. However, the other major leukocyte subsets, including T cells, natural killer cells, macrophages, and neutrophils were comparable in densities in chronic endometritis and nonpathological endometrium. The microvascular endothelium showed immunoreactivity to adhesion molecule selectin E and chemokine CXCL13 along with immunoreactivity to CXCL1 in the glandular epithelium in chronic endometritis, but not in the nonpathological endometrium. Lipopolysaccharide significantly induced surface selectin E expression and CXCL13 secretion in uterine microvascular endothelial cells, and CXCL1 secretion in endometrial epithelial cells in vitro. These findings indicated that the aberrant local microenvironment triggered possibly by bacterial infection has a role in selective extravasation of circulating B cells in chronic endometritis.

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“…An experienced pathologist later reconfirmed the cycle stage of each tissue sample. For the evaluation of mRNA and protein profile, the menstrual cycle stages of 64 samples were divided into seven groups by histopathology, based on well-established criteria [32]: early-proliferative (EP, n=8), mid-proliferative (MP, n=11), late-proliferative (LP, n=6), early-secretory (ES, n=10), mid-secretory (MS, n=11), late-secretory (LS, n=10) and menstrual (M, n=8). Endometrial tissues were either snap frozen on dry ice immediately after collection and stored at -80 C until RNA and protein extraction, or embedded carefully in OCT in plastic mold and frozen for IHC.…”

Section: Tissue Collectionmentioning

confidence: 99%

Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

Ponnampalam¹,

Gargett²,

Rogers³

2007

Eur J Immunol

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This study reports the discovery and steroid hormonal regulation of a novel glycosylated form of NKp30 in human endometrial epithelium. NKp30 is a member of the immunoglobulin superfamily and one of three existing natural cytotoxicity-triggering receptors. NKp30 is a glycosylated protein and is thought to be selectively expressed in resting and activated natural killer cells. The aims of the present study were to fully characterize NKp30 mRNA and protein in human endometrium during the menstrual cycle, and to investigate the hormonal regulation of NKp30. NKp30 mRNA was significantly up-regulated in fresh tissues during late secretory phase of the menstrual cycle. Interestingly, NKp30 mRNA was also present in clonally derived endometrial epithelial cells (CEE) in comparable amounts to fresh tissue. NKp30 protein was predominantly found in the endometrial glands and luminal epithelia of the secretory phase endometrium. Western blotting and de-glycosylation studies show that a novel glycosylated form of NKp30 is present in endometrial epithelium and that it can dimerize. Further phenotyping of CEE by flow cytometry revealed that they are CK8 + CD49f + NKp30 + CD45 -CD56 -. The data also show that transcription and translation of the novel form of NKp30 can be induced by progesterone treatment after 48 h in endometrial explants in vitro. This is the first report to show the presence of both NKp30 mRNA and a novel glycosylated form of NKp30 protein in endometrial epithelial cells.

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Dating the Endometrial Biopsy

Cited by 2,824 publications

References 11 publications

Loss of expression and nuclear/cytoplasmic localization of the FOXP1 forkhead transcription factor are common events in early endometrial cancer: relationship with estrogen receptors and HIF-1α expression

Loss of expression and nuclear/cytoplasmic localization of the FOXP1 forkhead transcription factor are common events in early endometrial cancer: relationship with estrogen receptors and HIF-1α expression

Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis

Identification and hormonal regulation of a novel form of NKp30 in human endometrial epithelium

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