Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner

Zhao, Chuanke; She, Tiantian; Wang, Lixin; Su, Yahui; Qu, Like; Gao, Yujing; Xu, Shuo; Cai, Shao‐Qing; Shou, Chengchao

doi:10.1016/j.lfs.2015.07.019

Cited by 52 publications

(31 citation statements)

References 41 publications

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“…It has higher cytotoxic effect than BS which shows that the apoptotic effect of BGT may be due to combination of BS and DS or DS alone. Recent study demonstrated that DS promotes autophagy in breast and gastric cancer cells by ROS dependent manner 40 . In the current study, we observed a strong growth inhibition when the cells were treated with a combination of both BS and DS.…”

Section: Discussionmentioning

confidence: 99%

Beta sitosterol and Daucosterol (phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells

Rajavel

Ramar

Archunan

et al. 2017

Sci Rep

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Lung cancer is the leading cause of cancer related deaths both in developed and developing countries. Since majority of the existing therapeutic methods harms both normal and malignant cells, a viable alternative is to switch into safe and beneficial traditional medicinal plants. Hence the present study was framed to identify selective anti-lung cancer agents from the medicinal plant Grewia tiliaefolia (GT). Cell viability experiments showed that benzene extract of GT (BGT) leaf effectively inhibited the growth of A549 cells, while being non-toxic to normal human lung L132 and PBMC cells. Ames and comet assays demonstrated that BGT is of non-mutagenic and non-genotoxic nature in untransformed cells. The hematological and histopathological profiles of the in vivo acute and sub-acute toxicity studies demonstrated that BGT is safe and tolerable. Importantly, western blot analysis and Annexin V-FITC staining confirmed that BGT promotes mitochondrial dependent apoptotic cell death in A549 cells by arresting cell cycle at G2/M phase. Bio-assay guided fractionation revealed the presence of phytosteols (β-sitosterol and daucosterol) which significantly inhibited the growth of A549 cells both alone and in combination. This study warrants that these phytosterols in alone or in combination can be considered as safe and potential drug candidates for lung cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Beta sitosterol and Daucosterol (phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells

Rajavel

Ramar

Archunan

et al. 2017

Sci Rep

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“…Sterols are the major terpenoids in I. indigotica, mainly comprising β-sitosterol and daucosterol 42 . β-Sitosterol was reported to play a critical role in curing lung inflammation 43 , while daucosterol can inhibit cancer cell proliferation 44 . A total of 59 genes in the present genome, which encoded 31 enzymes, were identified to be involved in terpenoid and sterol biosynthesis ( Supplementary Table S14).…”

Section: Identification Of Genes Involved In the Biosynthetic Pathwaymentioning

confidence: 99%

A chromosome-scale genome assembly of Isatis indigotica, an important medicinal plant used in traditional Chinese medicine

Kang

Yang

et al. 2020

Hortic Res

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Isatis indigotica (2n = 14) is an important medicinal plant in China. Its dried leaves and roots (called Isatidis Folium and Isatidis Radix, respectively) are broadly used in traditional Chinese medicine for curing diseases caused by bacteria and viruses such as influenza and viral pneumonia. Various classes of compounds isolated from this species have been identified as effective ingredients. Previous studies based on transcriptomes revealed only a few candidate genes for the biosynthesis of these active compounds in this medicinal plant. Here, we report a high-quality chromosome-scale genome assembly of I. indigotica with a total size of 293.88 Mb and scaffold N50 = 36.16 Mb using single-molecule real-time long reads and high-throughput chromosome conformation capture techniques. We annotated 30,323 highconfidence protein-coding genes. Based on homolog searching and functional annotations, we identified many candidate genes involved in the biosynthesis of main active components such as indoles, terpenoids, and phenylpropanoids. In addition, we found that some key enzyme-coding gene families related to the biosynthesis of these components were expanded due to tandem duplications, which likely drove the production of these major active compounds and explained why I. indigotica has excellent antibacterial and antiviral activities. Our results highlighted the importance of genome sequencing in identifying candidate genes for metabolite synthesis in medicinal plants. Data availabilityThe PacBio long reads and Illumina short reads were uploaded to the NCBI SRA database under BioProject PRJNA549758. The final chromosome-scale genome assembly was submitted to the NCBI with accession number VHIU00000000. The genome fasta and gff3 files were uploaded to Figshare.

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“…Following fixation with 4% paraformaldehyde for 20 min at room temperature, cells were permeabilized with 0.2% Triton X-100 for 30 min at room temperature. Immunofluorescent staining was performed as described previously (24). DNA was stained using DAPI (Sigma-Aldrich; Merck KGaA).…”

Section: Methodsmentioning

confidence: 99%

Negative feedback loop between ZBTB7A and TGF-β in breast cancer

et al. 2017

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Zinc finger and BTB domain containing 7A (ZBTB7A) is aberrantly expressed in breast cancer, but the involvement of ZBTB7A in breast cancer remains controversial. Transforming growth factor-β (TGF-β) is a pleiotropic cytokine which promotes breast cancer metastasis. ZBTB7A and TGF-β are important factors in tumor development. However, the association between ZBTB7A and TGF-β in breast cancer remains unknown. The results of the present study revealed that TGF-β1 induced the expression of ZBTB7A via the phosphoinositide 3-kinase-protein kinase B signaling pathway in human breast cancer cells, and ZBTB7A inhibited the expression of TGF-β1 through indirectly suppressing the promoter activity of TGF-β1. Furthermore, no significant correlation between the expression of ZBTB7A and TGF-β1 were identified in breast cancer tissues using tissue microarray assay and human cancer genomics analysis. These results have identified a negative feedback loop between ZBTB7A and TGF-β signaling, suggesting ZBTB7A as a potential modulator of breast cancer metastasis. Thus, the results of the present study suggested that ZBTB7A is a potential prognostic biomarker for breast cancer.

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Daucosterol inhibits cancer cell proliferation by inducing autophagy through reactive oxygen species-dependent manner

Cited by 52 publications

References 41 publications

Beta sitosterol and Daucosterol (phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells

Beta sitosterol and Daucosterol (phytosterols identified in Grewia tiliaefolia) perturbs cell cycle and induces apoptotic cell death in A549 cells

A chromosome-scale genome assembly of Isatis indigotica, an important medicinal plant used in traditional Chinese medicine

Negative feedback loop between ZBTB7A and TGF-β in breast cancer

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