Daunomycin, an antitumor DNA intercalator, influences histone–DNA interactions

Wójcik, Krzysztof; Zarębski, Mirosław; Cossarizza, Andrea; Dobrucki, Jurek

doi:10.4161/cbt.25328

Cited by 30 publications

(33 citation statements)

References 30 publications

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“…This phenomenon might lead to phosphorylation of the histone H2AX (Wojcik et al, 2013), which is linked to the inhibition of topoisomerase II and the subsequent ATM/ATR-regulated DNA damage-dependent stress responses (Shiloh, 2001), such as the accumulation of p53 protein and the phosphorylation of Chk-1 (Damrot et al, 2006), and mitochondrial abnormalities (Sahin et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Comparative endothelial profiling of doxorubicin and daunorubicin in cultured endothelial cells

Wójcik

Buczek

Majzner

et al. 2015

Toxicology in Vitro

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Section: Discussionmentioning

confidence: 99%

Comparative endothelial profiling of doxorubicin and daunorubicin in cultured endothelial cells

Wójcik

Buczek

Majzner

et al. 2015

Toxicology in Vitro

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“…Before adding the primary antibody Ab0 (which binds the histone H1), incubation in 3% bovine serum albumin was performed in order to prevent nonspecific binding [24]. After 1h of incubation with Ab0, cells were washed with phosphatebuffered saline and then, the secondary antibody Ab1 labeled with AF405 was added for the next 1h incubation.…”

Section: A Cell Culture and Immunofluorescencementioning

confidence: 99%

Measurements on MIMO-FRET Nano-Networks Based on Alexa Fluor Dyes

Wójcik

Solarczyk

Kułakowski

2015

IEEE Trans. Nanotechnology

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Nanocommunication has gained significant attention in the last few years, as a means to establish information transfer between future nanomachines. Comparing with other communication techniques for nanoscale (calcium ions signaling, molecular or catalytic nanomotors, pheromones propagation, bacteria-based communication), the phenomenon called Förster Resonance Energy Transfer (FRET) offers significantly smaller propagation delays and high channel throughput. In this paper, we report our recent experiments on FRET-based nanonetworks performed in the Laboratory of Cell Biophysics of the Jagiellonian University, Kraków. We propose to use Alexa Fluor dyes as nano transmitters and receivers, as they enable to create multiple-input multioutput (MIMO) FRET communication channels and thus enhance FRET efficiency. We measure FRET efficiency values, calculate bit error rates for the measured scenarios, and extend the calculations to consider a general case of MIMO (n, m) FRET channels.

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“…It is important to note, however, that live cells actively remove AO (15); therefore, the color of the fluorescence emission of acidic vesicles probably depends on the balance between the pH-dependent influx of the dye, a passive diffusion through the vesicular membrane in the direction of the concentration gradient, and an active removal of AO from the cytoplasm to the extracellular space. It is also noted that at this concentration AO induced chromatin aggregation ( Figs. 2A and 2C), most likely due to dissociation of the linker (H1) histones (16,17).…”

Section: Tracking Acidic Vesicles Loaded With Aomentioning

confidence: 99%

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

2014

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Acidic vesicles can be imaged and tracked in live cells after staining with several low molecular weight fluorescent probes, or with fluorescently labeled proteins. Three fluorescent dyes, acridine orange, LysoTracker Red DND-99, and quinacrine, were evaluated as acidic vesicle tracers for confocal fluorescence imaging and quantitative analysis. The stability of fluorescent signals, achievable image contrast, and phototoxicity were taken into consideration. The three tested tracers exhibit different advantages and pose different problems in imaging experiments. Acridine orange makes it possible to distinguish acidic vesicles with different internal pH but is fairly phototoxic and can cause spectacular bursts of the dye-loaded vesicles. LysoTracker Red is less phototoxic but its rapid photobleaching limits the range of useful applications considerably. We demonstrate that quinacrine is most suitable for long-term imaging when a high number of frames is required. This capacity made it possible to trace acidic vesicles for several hours, during a process of drug-induced apoptosis. An ability to record the behavior of acidic vesicles over such long periods opens a possibility to study processes like autophagy or long-term effects of drugs on endocytosis and exocytosis. V C 2014International Society for Advancement of Cytometry

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Daunomycin, an antitumor DNA intercalator, influences histone–DNA interactions

Cited by 30 publications

References 30 publications

Comparative endothelial profiling of doxorubicin and daunorubicin in cultured endothelial cells

Comparative endothelial profiling of doxorubicin and daunorubicin in cultured endothelial cells

Measurements on MIMO-FRET Nano-Networks Based on Alexa Fluor Dyes

Evaluation of acridine orange, LysoTracker Red, and quinacrine as fluorescent probes for long‐term tracking of acidic vesicles

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