Daunomycin-loaded superparamagnetic iron oxide nanoparticles: Preparation, magnetic targeting, cell cytotoxicity, and protein delivery research

Abstract

The clinical use of daunomycin is restricted by dose-dependent toxicity and low specificity against cancer cells. In the present study, modified superparamagnetic iron oxide nanoparticles were employed to load daunomycin and the drug-loaded nanospheres exhibited satisfactory size and smart pH-responsive release. The cellular uptake efficiency, targeted cell accumulation, and cell cytotoxicity experimental results proved that the superparamagnetic iron oxide nanoparticle-loading process brings high drug targeting without decreasing the cytotoxicity of daunomycin. Moreover, a new concern for the evaluation of nanophase drug delivery's effects was considered, with monitoring the interactions between human serum albumin and the drug-loaded nanospheres. Results from the multispectroscopic techniques and molecular modeling calculation elucidate that the drug delivery has detectable deleterious effects on the frame conformation of protein, which may affect its physiological function.

Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity

“…Current investigations have also synthesized IONPs employing different shells with anticancer agents conventionally administered, such as β-cyclodextrin [ 135 ], carmustine [ 136 ], cetuximab [ 137 , 138 , 139 ], cytarabine [ 140 ], daunomycin [ 141 ], docetaxel [ 142 , 143 ], epirubicin [ 144 ], 5-fluorouracil [ 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 ], gemcitabine [ 22 , 154 , 155 , 156 , 157 , 158 ], methotrexate [ 159 , 160 , 161 ], mitoxantrone [ 162 , 163 , 164 , 165 ] and paclitaxel [ 27 , 104 , 166 , 167 , 168 , 169 , 170 , 171 , 172 ]. It is noteworthy, however, that these nanosystems demonstrate magnetic properties only in the presence of external magnetic fields to prevent agglomerations of nanoparticles [ 111 ] and to allow a satisfactory performance in the target sites.…”

<p>Rotating Magnetic Nanoparticle Clusters as Microdevices for Drug Delivery</p>

“…11 Considering MNP modeling relevant to cancer, studies have shown that a variety of chemotherapeutic drugs can be bound to, and transported by MNPs. [60][61][62][63] MNP-chemotherapy constructs have demonstrated growth-inhibitory effects in vitro and in animal studies for many types of cancer cells, including glioblastoma. 2,[64][65][66] Ingenious larger nanostructures ("micro-robots") which can be actuated by external magnetic fields have also been described, with the goal of targeting drug delivery.…”

Calcium carbonate end-capped, folate-mediated Fe3O4@mSiO2 core-shell nanocarriers as targeted controlled-release drug delivery system

“…Fe 3 O 4 Nps were synthesized by a coprecipitation of ferric and ferrous chlorides in alkaline medium following the method we have previously reported. 22 Fe 3 O 4 @SiO 2 core-shell nanocomposites were fabricated via sol-gel process of hydrolysis and condensation of TEOS. Briefly, 50 mg Fe 3 O 4 compound was transferred to a beaker, dispersed in 30 ml water, assisted with ultrasounds.…”