Day-to-day reproducibility of prolonged ambulatory colonic manometry in healthy subjects

Background An important limitation of gastrointestinal motility testing is high variability. Conditions that could contribute to variability, including the duration of pretest fasting and time of day, are rarely reported and have not been examined systematically. This study aimed to explore whether these conditions, as well as age, sex, and strain of mice, affect the results of a standard laboratory test of upper gastrointestinal motility. Methods Male and female 8‐week‐old C57BL/6J mice received a gastric gavage of fluorescein isothiocyanate (FITC)‐conjugated dextran. FITC‐dextran distribution was measured 30 minutes later. Mean geometric centers (MGCs) were calculated to determine the effects of short versus prolonged fasting and morning versus afternoon testing. The influence of age was assessed in 2‐ to 10‐week‐old animals, and the influence of strain was determined in C57BL/6J, BALB/c, and CD‐1 mice. Key results Motility was sexually dimorphic. MGC progressed 19% further in 8‐week‐old C57BL/6J males versus females when tested in the morning after a short fast. Similar patterns were observed in morning or afternoon testing after overnight fasting. In males, motility was unaffected by time of day; however, MGC progressed 31% further in females tested in the afternoon versus morning after a short fast. Sex differences also were present in CD‐1 but not BALB/c mice. Testing in neonates revealed strikingly low variability and no sex differences. Conclusions & inferences Fasting duration, time of day, age, sex, and strain of mice all influence upper gastrointestinal motility testing. Sex differences are not present in neonatal pups, but develop soon after weaning.

show abstract

“…Gastrointestinal motility measurements are complicated by high variability in both humans and animal models. Here, we highlight three key points.…”

Section: Discussionmentioning

confidence: 99%

Sexual dimorphism in upper gastrointestinal motility is dependent on duration of fast, time of day, age, and strain of mice

Soni

Halder

Conner

et al. 2019

Neurogastroenterology Motil

View full text Add to dashboard Cite

show abstract

“…Nonetheless, in 2010, Rao et al. investigated the day‐to‐day reproducibility of prolonged 24‐hour CM performed 2 weeks apart in healthy adults and demonstrated that the study findings were reproducible, particularly for the assessment of key physiologic changes, including HAPCs . In their study, colonic motility catheters were placed under minimal sedation unlike our own in which general anesthesia was used for catheter placement.…”

Section: Discussionmentioning

confidence: 94%

Prospective evaluation of same day versus next day colon manometry results in children with medical refractory constipation

Arbizu

Nurko

Heinz

et al. 2017

Neurogastroenterology Motil

View full text Add to dashboard Cite

Background We evaluated the change on colon manometry (CM) parameters and interpretation comparing results when the study is performed the same day after the motility catheter is placed under anesthesia or the following day. Methods CM catheter was placed with colonoscopy under anesthesia and recorded on day 1 and repeated on day 2. Study parameters including motility index (MI) during fasting, post-prandial and post-Bisacodyl challenge phase; gastrocolonic response; number, presence and propagation of high amplitude propagating contractions (HAPCs); and, study interpretation were compared between both days. Key Results MI (fasting, post-Bisacodyl phase, p<0.05), HAPC number (10.1 vs. 6.6, p=0.01) and proportion of patients having HAPCs (92% vs. 70%, p=0.002) was significantly higher on day 2 vs. day 1. HAPC propagation improved on day 2 vs. day 1 (fully propagated, 49% vs. 37%; partially propagated, 43% vs. 33%; absent 8% vs. 30%). Study interpretation changed from day 1 to day 2. On day 1, 37% had a normal study and 63% had an abnormal study. On day 2, all patients with a normal study on day 1 remained normal, and patients with an abnormal study on day 1, 53% remained abnormal and 47% had a normal study. Conclusions & Inferences CM parameters are affected the day catheter is placed with colonoscopy under anesthesia. The number, presence and propagation of HAPCs was significantly higher/improved on day 2 compared to day 1. Overall, CM interpretation changed from abnormal to normal from day 1 to day 2 in 47% of the patients.

show abstract

“…It may also be useful to examine the optimal time for treatment. Since there is a lack of waking patterns in colonic motility in patients with STC , TES in the morning may be more effective.…”

Section: Discussionmentioning

confidence: 99%

Home-Based Transabdominal Interferential Electrical Stimulation for Six Months Improves Paediatric Slow Transit Constipation (STC)

Yik

Hutson

2018

Neuromodulation: Technology at the Neural Interface

View full text Add to dashboard Cite

Day-to-day reproducibility of prolonged ambulatory colonic manometry in healthy subjects

Abstract: Background-Although colonic manometry provides useful information regarding colonic physiology, considerable variability has been reported both for regional motility and manometric patterns. Whether colonic manometry is reproducible is not known.

Cited by 16 publications

References 42 publications

Sexual dimorphism in upper gastrointestinal motility is dependent on duration of fast, time of day, age, and strain of mice

Sexual dimorphism in upper gastrointestinal motility is dependent on duration of fast, time of day, age, and strain of mice

Prospective evaluation of same day versus next day colon manometry results in children with medical refractory constipation

Home-Based Transabdominal Interferential Electrical Stimulation for Six Months Improves Paediatric Slow Transit Constipation (STC)

Contact Info

Product

Resources

About