Day to Day Variability and Reliability of Blood Oxidative Stress Markers within a Four-Week Period in Healthy Young Men

Abstract

The present study aimed to determine the day to day variability and reliability of several blood oxidative stress markers at rest in a healthy young cohort over a four-week period. Twelve apparently healthy resistance trained males (24.6 ± 3.0 yrs) were tested over 7 visits within 4 weeks with at least 72 hrs between visits at the same time of day. Subjects rested 30 minutes prior to blood being obtained by vacutainer. Results. The highest IntraClass correlations (ICC's) were obtained for protein carbonyls (PC) and oxygen radical absorbance capacity (ORAC) (PC = 0.785 and ORAC = 0.780). Cronbach's α reliability score for PC was 0.967 and for ORAC was 0.961. The ICC's for GSH, GSSG, and the GSSG/TGH ratio ICC were 0.600, 0.573, and 0.570, respectively, with Cronbach's α being 0.913, 0.904, and 0.903, respectively. Xanthine oxidase ICC was 0.163 and Cronbach's α was 0.538. Conclusions. PC and ORAC demonstrated good to excellent reliability while glutathione factors had poor to excellent reliability. Xanthine oxidase showed poor reliability and high variability. These results suggest that the PC and ORAC markers were the most stable and reliable oxidative stress markers in blood and that daily changes across visits should be considered when interpreting resting blood oxidative stress markers.

Candidate proteomic biomarkers in systemic sclerosis discovered using mass-spectrometry: an update of a systematic review (2014–2020)

“…An important aspect is the fact that markers of oxidative stress were found to be upregulated in many other pathologies, not only autoimmune, but also in various cancers or in elders [21], [22]. In addition, there are numerous reports in which oxidative stress molecules appear to have wide day to day fluctuations [23]. As such, evaluating enzymes that are upstream in the metabolic chain could be more useful, or at least as useful as evaluation of the end-products.…”

Intra-Individual Variation and Reliability of Biomarkers of the Antioxidant Defense System by Considering Dietary and Lifestyle Factors in Premenopausal Women

“…The reported ICC values in the literature varied greatly. For example, in studies which did not focus on circadian effects or changes within menstrual cycle, the following ICC values were reported: bilirubin 0.4–0.8 [ 21 ], uric acid 0.7–1.0 [ 21 ], vitamin C 0.4–0.8 [ 20 , 23 ], α- and/or γ-tocopherol 0.1–0.9 [ 19 , 23 , 26 ], retinol 0.4–0.9 [ 19 , 20 , 26 ], carotenoids 0.2–0.8 [ 19 , 20 , 23 ], total antioxidant capacity (equivalent to TEAC) 0.01 [ 66 ] and reduced/oxidized glutathione 0.6 [ 25 ]. Overall, the ICC values of the present study are in the range of previously reported ICC values, with the exception of glutathione.…”

“…Overall, the ICC values of the present study are in the range of previously reported ICC values, with the exception of glutathione. In this particular study, the ICC for reduced/oxidized glutathione, not total glutathione, was calculated in a population of 12 resistance-trained men with 7 visits within one month [ 25 ]. Besides the fact that the available studies differ considerably in their designs, we want to emphasize that ICC and CV are population-specific estimates and thus not pertinent without restrictions to population groups with inherently different levels of heterogeneity [ 67 ].…”

“…Data on intra-individual variation and/or the intraclass correlation coefficient (ICC) as indicator of relative reliability were reported for some blood antioxidant biomarkers [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ]. However, several previous reliability studies represent secondary analyses, gave no information on health status of the subjects, restricted fruit and vegetable consumption, included only subjects with unchanged vitamin intake, included supplement users, used non-fasting blood samples or investigated predominantly non-enzymatic biomarkers with two measuring points several years apart.…”

The Effect of Smoking on Biomarkers of (Anti) oxidant Status

“…In biomarker studies a number of modifying factors have to be taken into account such as storage conditions [10], the influence of post-prandial effects [11], circadian rhythm [12,13], body mass index [14] and the smoking behavior. In the present study the influence of smoking on number biomarkers of oxidative stress was investigated in healthy young men.…”