Daytime light enhances the amplitude of circadian output in a diurnal mammal

Abstract

Mammalian circadian rhythms are orchestrated by a master pacemaker in the hypothalamic suprachiasmatic nuclei (SCN), which receives information about the 24 h light:dark cycle from the retina. The accepted function of this light signal is to reset circadian phase in order to ensure appropriate synchronisation with the celestial day. Here, we ask whether light also impacts another key property of the circadian oscillation, its amplitude. To this end, we measured rhythms in behavioural activity and body temperature, and SCN electrophysiological activity in the diurnal murid rodent Rhabdomys pumilio following stable entrainment to 12:12 light:dark cycles at 4 different daytime intensities (ranging from 12.77 to 14.80 log melanopsin effective photons/cm 2 /s). Rhabdomys showed strongly diurnal activity and body temperature rhythms in all conditions, but measures of rhythm robustness were positively correlated with daytime irradiance under both entrainment and subsequent free run. Whole-cell and extracellular recordings of electrophysiological activity in ex vivo SCN revealed substantial differences in electrophysiological activity between dim and bright light conditions. At lower daytime irradiance, daytime peaks in SCN spontaneous firing rate and membrane depolarisation were substantially depressed, leading to an overall marked reduction in the amplitude of circadian rhythms in spontaneous activity. Our data reveal a previously unappreciated impact of daytime light intensity on SCN physiology and the amplitude of circadian rhythms, and highlight the potential importance of daytime light exposure for circadian health.

Postoperative cognitive dysfunction: spotlight on light, circadian rhythms, and sleep

“…This environment delivering low light levels during the day may prevent patients from maintaining stable circadian rhythms ( Durrington et al, 2017 ). In fact, the lack of circadian-effective daytime light has been shown to be associated with decreased circadian rhythm amplitude ( Bano-Otalora et al, 2020 ), poor sleep quality, and delayed sleep onset ( Blume et al, 2019 ). Bernhofer et al also found that low daytime light levels in hospitals predicted sleep fragmentation and mood disturbances among patients ( Bernhofer et al, 2014 ).…”

Daily electrical activity in the master circadian clock of a diurnal mammal

“…Rhabdomys pumilio (the four striped mouse) represents an excellent opportunity to address this question. This species is strongly diurnal (Dewsbury & Dawson, 1979;Schumann et al, 2005;Bano-Otalora et al, 2020) and is a murid rodent, facilitating comparison with established findings from closely related nocturnal species (mice and rats). We adopted a parallel approach of experimental recording and advanced computational modelling to understand the R. pumilio SCN.…”