Daytime restricted feeding modifies the daily variations of liver gluconeogenesis: Adaptations in biochemical and endocrine regulators

Pérez-Mendoza, Moisés; Rivera-Zavala, Julieta Berenice; Dı́az-Muñoz, Mauricio

doi:10.3109/07420528.2014.908898

Cited by 25 publications

(47 citation statements)

References 39 publications

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“…Hence, based on reports of physiological consequences of continuous UAG infusion, it is suggested that the high concentration of UAG (which we found to be associated with dRF) could improve glucose metabolism and inhibit lipolysis, thus displaying effects with hypoglycaemic properties (42). Indeed, we have observed a hypoglycaemic state in rats under dRF (43).…”

Section: Discussionsupporting

confidence: 52%

“…The increase of GH seen after feeding (ZT 6 h) in the dRF group is not easy to explain given the proven inhibitory action of insulin on GH secretion . Particularly, the dRF paradigm promotes a large peak of insulin after mealtime at ZT 6 h . A tentative interpretation is that the rheostatic regulation associated with dRF could be altering the signalling by JAK2 and STAT5B, which mediate the inhibitory action of insulin on GH secretion .…”

Section: Discussionmentioning

confidence: 99%

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Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Arellanes-Licea¹,

Báez-Ruíz²,

Carranza³

et al. 2014

J Neuroendocrinology

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Daytime restricted feeding promotes the re-alignment of the food entrained oscillator (FEO). Endocrine cues which secretion is regulated by the transition of fasting and feeding cycles converge in the FEO. The present study aimed to investigate the ghrelin, growth hormone (GH) and insulin-like growth factor (IGF)-1 system because their release depends on rhythmic and nutritional factors, and the output from the system influences feeding and biochemical status. In a daily sampling approach, rats that were fed ad lib. were compared with rats on a reversed (daytime) and restricted feeding schedule by 3 weeks (dRF; food access for 2 h), also assessing the effect of acute fasting and refeeding. We undertook measurements of clock protein BMAL1 and performed somatometry of peripheral organs and determined the concentration of total, acylated and unacylated ghrelin, GH and IGF-1 in both serum and in its main synthesising organs. During dRF, BMAL1 expression was synchronised to mealtime in hypophysis and liver; rats exhibited acute hyperphagia, stomach distension with a slow emptying, a phase shift in liver mass towards the dark period and decrease in mass perigonadal white adipose tissue. Total ghrelin secretion during the 24-h period increased in the dRF group as a result of elevation of the unacylated form. By contrast, GH and IGF-1 serum concentration fell, with a modification of GH daily pattern after mealtime. In the dRF group, ghrelin content in the stomach and pituitary GH content decreased, whereas hepatic IGF-1 remained equal. The daily patterns and synthesis of these hormones had a rheostatic adaptation. The endocrine adaptive response elicited suggests that it may be associated with the regulation of metabolic, behavioural and physiological processes during the paradigm of daytime restricted feeding and associated FEO activity.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Arellanes-Licea¹,

Báez-Ruíz²,

Carranza³

et al. 2014

J Neuroendocrinology

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“…DRF promotes a phase shift in BMAL1 and PER1 (2,3) , an oxidised redox state in the mitochondria and cytosol (7) , higher levels of SIRT1 in hepatic cytosol before mealtime, and an elevated presence of cytosolic PGC-1α in the 24-h profile (14) . All these changes are associated with an enhanced 'fasting' response in DRF rats at times before food access (2,3,7,14) . In our results, the nucleus:cytosol ratio of SIRT1 exhibits a peak at the end of the dark phase ( Fig.…”

Section: Circadian Aspectsmentioning

confidence: 99%

“…2(a)) (7) . DRF is characterised by the presence of food anticipatory behaviour (FAB) before mealtime, when the metabolic fasting response is more active in the liver (2,(7)(8)(9)14) . The metabolic products of lipid catabolism, KB (Fig.…”

Section: Circadian Aspectsmentioning

confidence: 99%

Daytime restricted feeding modifies the daily regulation of fatty acidβ-oxidation and the lipoprotein profile in rats

et al. 2017

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Daytime restricted feeding (2 h of food access from 12.00 to 14.00 hours for 3 weeks) is an experimental protocol that modifies the relationship between metabolic networks and the circadian molecular clock. The precise anatomical locus that controls the biochemical and physiological adaptations to optimise nutrient use is unknown. We explored the changes in liver oxidative lipid handling, such as β-oxidation and its regulation, as well as adaptations in the lipoprotein profile. It was found that daytime restricted feeding promoted an elevation of circulating ketone bodies before mealtime, an altered hepatic daily rhythmicity of 14CO2 production from radioactive palmitic acid, and an up-regulation of the fatty acid oxidation activators, the α-subunit of AMP-activated protein kinase (AMPK), the deacetylase silent mating type information regulation homolog 1, and the transcriptional factor PPARγ-1α coactivator. An increased localisation of phosphorylated α-subunit of AMPK in the periportal hepatocytes was also observed. Liver hepatic lipase C, important for lipoprotein transformation, showed a change of daily phase with a peak at the time of food access. In serum, there was an increase of LDL, which was responsible for a net elevation of circulating cholesterol. We conclude that our results indicate an enhanced fasting response in the liver during daily synchronisation to food access, which involves altered metabolic and cellular control of fatty acid oxidation as well a significant elevation of serum LDL. These adaptations could be part of the metabolic input that underlies the expression of the food-entrained oscillator.

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“…9 Besides its important biochemical role, GDH has been implicated in pathological conditions such as cancer, hyperinsulinism, and ethanol-induced cell necrosis. [10][11][12] Using a food restriction protocol (2 h of daytime food access for three weeks), we have reported a set of biochemical adaptations in liver mitochondrial activity and metabolic nitrogen-handling responses that could influence GDH functional properties: (1) enhanced mitochondrial synthesis of ATP, 13 (2) reduced mitochondrial lipid peroxidative activity, 14 (3) increased gluconeogenesis and ureagenesis, 15,16 and (4) modified activity and expression of glutamine synthetase and GABA transaminase. 17,18 This protocol has also been used to study an alternative circadian clock, different from the suprachiasmatic nucleus, which is entrained by food access (known as the food entrainable oscillator, FEO).…”

Section: Introductionmentioning

confidence: 99%

Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location

Vázquez‐Martínez¹,

Méndez²,

Turrubiate³

et al. 2017

Exp Biol Med (Maywood)

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Impact statementFor the first time, we are reporting the changes in daily rhythmicity of glutamate dehydrogenase (GDH) mRNA, protein and activity that occur in the liver during the expression of the food entrained oscillator (FEO). These results are part of the metabolic adaptations that modulate the hepatic timing system when the protocol of daytime restricted feeding is applied. As highlight, it was demonstrated higher GDH protein and activity in the mitochondrial fraction. These results contribute to a better understanding of the influence of the FEO in the energy and nitrogen handling in the liver. They could also be significant in the pathophysiology of hepatic diseases related with circadian abnormalities. AbstractGlutamate dehydrogenase is an important enzyme in the hepatic regulation of nitrogen and energy metabolism. It catalyzes one of the most relevant anaplerotic reactions. Although its relevance in liver homeostasis has been widely described, its daily pattern and responsiveness to restricted feeding protocols has not been studied. We explored the daily variations of liver glutamate dehydrogenase transcription, protein, activity, and histochemical and subcellular location in a protocol of daytime food synchronization in rats. Restricted feeding involved food access for 2 h each day for three weeks. Control groups included food ad libitum as well as acute fasting (21 h fasting) and refeeding (22 h fasting followed by 2 h of food access). Glutamate dehydrogenase mRNA, protein, activity, and histological location were measured every 3 h by qPCR, Western blot, spectrophotometry, and immunohistochemistry, respectively, to generate 24-h profiles. Restricted feeding promoted higher levels of mitochondrial glutamate dehydrogenase protein and activity, as well as a loss of 24-h rhythmicity, in comparison to ad libitum conditions. The rhythmicity of glutamate dehydrogenase activity detected in serum was changed. The data demonstrated that daytime restricted feeding enhanced glutamate dehydrogenase protein and activity levels in liver mitochondria, changed the rhythmicity of its mRNA and serum activity, but without effect in its expression in hepatocytes surrounding central and portal veins. These results could be related to the adaptation in nitrogen and energy metabolism that occurs in the liver during restricted feeding and the concomitant expression of the food entrainable oscillator.Keywords: Glutamate dehydrogenase, mitochondria, food synchronization, daily variations Experimental Biology and Medicine 2017; 242: 945-952.

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Daytime restricted feeding modifies the daily variations of liver gluconeogenesis: Adaptations in biochemical and endocrine regulators

Cited by 25 publications

References 39 publications

Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Daytime restricted feeding modifies the daily regulation of fatty acidβ-oxidation and the lipoprotein profile in rats

Restricted feeding modulates the daily variations of liver glutamate dehydrogenase activity, expression, and histological location

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