DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation

Abstract

Meiotic progression requires exquisitely coordinated translation of maternal messenger (m)RNA that has accumulated during oocyte growth. A major regulator of this program is the cytoplasmic polyadenylation element binding protein 1 (CPEB1). However, the temporal pattern of translation at different meiotic stages indicates the function of additional RNA binding proteins (RBPs). Here, we report that deleted in azoospermia-like (DAZL) cooperates with CPEB1 to regulate maternal mRNA translation. Using a strategy that monitors ribosome loading onto endogenous mRNAs and a prototypic translation target, we show that ribosome loading is induced in a DAZL-and CPEB1-dependent manner, as the oocyte reenters meiosis. Depletion of the two RBPs from oocytes and mutagenesis of the 3′ untranslated regions (UTRs) demonstrate that both RBPs interact with the Tex19.1 3′ UTR and cooperate in translation activation of this mRNA. We observed a synergism between DAZL and cytoplasmic polyadenylation elements (CPEs) in the translation pattern of maternal mRNAs when using a genome-wide analysis. Mechanistically, the number of DAZL proteins loaded onto the mRNA and the characteristics of the CPE might define the degree of cooperation between the two RBPs in activating translation and meiotic progression.

A MAPK cascade couples maternal mRNA translation and degradation to meiotic cell cycle progression in mouse oocytes

“…We provide evidence that the insufficient accumulation of these maternal factors is the primary reason for the developmental defects seen in ERK1/2-inhibited oocytes. Studies on mouse oocytes indicate that DAZL functions as a CPEB1-downstream translational activator, and several putative targets, including Tex19.1, Tpx2 and Dazl itself, have been identified (Sousa Martins et al, 2016). In this study, we add Btg4 to the list of DAZL-regulated maternal transcripts.…”

Dynamic Changes in Proteome during Yak Oocyte Maturation Analyzed Using iTRAQ Technology

“…Oocytes are important female germ cells, and many protein changes are involved in in vitro maturation. Therefore, it is crucial to study the dynamic changes in proteins during each period of oocyte maturation to improve yak fertility [ 9 , 10 , 11 ]. iTRAQ is a protein quantification technology based on tandem mass spectrometry that can identify almost all proteins in an animal [ 12 , 13 ].…”