Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs

Lee, Kyung Ho; Lee, Seongju; Kim, Byunghyuk; Chang, Sunghoe; Kim, Soo Woong; Paick, Jae‐Seung; Rhee, Kunsoo

doi:10.1038/sj.emboj.7601304

Cited by 58 publications

(65 citation statements)

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“…mDAZL also interacts in vitro with dynein light chain, a component of the microtubule-associated dyneindynactin motor complex which has an important role in mRNA localization (Lee et al 2006). Indeed, when mDAZL was ectopically expressed in cultured somatic cells, it was able to regulate the cytoplasmic distribution of reporter mRNAs containing either the CDC25C (but Model for DAZL-mediated translational stimulation of target mRNAs.…”

Section: Mechanism Of Dazl-mediated Translational Stimulationmentioning

confidence: 95%

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Brook¹,

Smith²,

Gray³

2009

Reproduction

111

114

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Gametogenesis is a highly complex process that requires the exquisite temporal, spatial and amplitudinal regulation of gene expression at multiple levels. Translational regulation is important in a wide variety of cell types but may be even more prevalent in germ cells, where periods of transcriptional quiescence necessitate the use of post-transcriptional mechanisms to effect changes in gene expression. Consistent with this, studies in multiple animal models have revealed an essential role for mRNA translation in the establishment and maintenance of reproductive competence. While studies in humans are less advanced, emerging evidence suggests that translational regulation plays a similarly important role in human germ cells and fertility. This review highlights specific mechanisms of translational regulation that play critical roles in oogenesis by activating subsets of mRNAs. These mRNAs are activated in a strictly determined temporal manner via elements located within their 3 0 UTR, which serve as binding sites for trans-acting factors. While we concentrate on oogenesis, these regulatory events also play important roles during spermatogenesis. In particular, we focus on the deleted in azoospermia-like (DAZL) family of proteins, recently implicated in the translational control of specific mRNAs in germ cells; their relationship with the general translation initiation factor poly(A)-binding protein (PABP) and the process of cytoplasmic mRNA polyadenylation.

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Section: Mechanism Of Dazl-mediated Translational Stimulationmentioning

confidence: 95%

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Brook¹,

Smith²,

Gray³

2009

Reproduction

111

114

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“…In addition to this interaction with PABP, DAZ family proteins also interact with a number of other proteins, including DAZAP1, DAZAP2, PUM2, hQK3, DZIP1, DZIP2, DZIP3, and Dynein light chain, many of which are RNA binding proteins as well as being capable of forming hetero-and homodimers with other DAZ family members (Ruggiu and Cooke 2000;Tsui et al 2000a;Xu et al 2001;Moore et al 2003Moore et al , 2004Urano et al 2005;Lee et al 2006). The functional significance of many of these interactions remains to be clarified in vivo, although it seems probable that interactions with different proteins will have differing effects on post-transcription regulation of mRNAs, e.g., an interaction with PABP promotes translation, interaction with Pum2 and DAZAP1 (Moore et al 2003;Urano et al 2005;Morton et al 2006) may be involved in translational repression, and interactions with dynein light chain may be required for specific localization of messages (Tsui et al 2000a;Lee et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Translation of the synaptonemal complex component Sycp3 is enhanced in vivo by the germ cell specific regulator Dazl

Reynolds¹,

Collier²,

Bingham³

et al. 2007

RNA

117

139

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DAZ-related genes are essential for gametogenesis in diverse metazoa: in human males, a loss of DAZ genes is associated with infertility. These genes, expressed only in germ cells, regulate the translation of a yet undefined set of specific transcripts, and loss of function results in numerous defects throughout the mitotic and meiotic process of germ cell development. In a mouse model, absence of the autosomal Dazl gene results in a final block at zygotene of meiotic prophase. Sycp3 is also essential for meiosis, specifically for the formation of the synaptonemal complex lateral element with a mouse knockout model displaying a block in meiotic prophase similar to the Dazl knock out. Sycp3 was identified as a potential target for translational regulation by Dazl in male mouse germ cells. This was confirmed by both RNA binding and translation assays. In the Dazl knockout mouse model, Sycp3 protein levels were decreased, indicating that Dazl is required for efficient translation of the Sycp3 mRNA in vivo. Taken together these data support Sycp3 as a biologically relevant target of Dazl-mediated translation in mammals. This suggests that azoospermia associated with a decrease in DAZ gene function in humans may in part be a consequence of failure at synapsis caused by reduced levels of SYCP3 protein.

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“…Among DAZ (deleted in azoospermia) family of RBPs that were extensively studied in males (Lee et al 2006), DAZL is the only family member detected in the cytoplasm of mouse oocytes at all oogenic stages (Ruggiu et al 1997). In male germ cells, DAZL binds to a subset of mRNAs via a specific DAZL-binding motif and regulates their translation (Lee et al 2006). In addition, it interacts with the dynein-dynactin complex suggesting that it controls their transport and recruitment to polysomes.…”

Section: Rbps and Translational Controlmentioning

confidence: 99%

“…Similarly, absence of Dazl results in a complete loss of female germ cells before birth (Ruggiu et al 1997; Table 1). Among DAZ (deleted in azoospermia) family of RBPs that were extensively studied in males (Lee et al 2006), DAZL is the only family member detected in the cytoplasm of mouse oocytes at all oogenic stages (Ruggiu et al 1997). In male germ cells, DAZL binds to a subset of mRNAs via a specific DAZL-binding motif and regulates their translation (Lee et al 2006).…”

Section: Rbps and Translational Controlmentioning

confidence: 99%

“…On the other hand, DAZL has been shown in various human tumoral cell lines to exhibit a cytoplasmic granular expression (http://www.proteinatlas.org/ ENSG00000092345/subcellular). In addition, when overexpressed in HeLa cells, DAZL concentrates in cytoplasmic RNA granules that most probably correspond to SGs because they contain TIA1, a characteristic marker of SGs (Lee et al 2006; Box 1). These data strongly suggest that in somatic cells, DAZL participates to the storage of its target mRNAs at specific cytoplasmic sites.…”

Section: Rbps and Translational Controlmentioning

confidence: 99%

See 1 more Smart Citation

RNA-binding proteins, RNA granules, and gametes: is unity strength?

Nguyen-Chi¹,

Morello²

2011

Reproduction

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Changes in mRNA translation and degradation represent post-transcriptional processes operating during gametogenesis and early embryogenesis to ensure regulated protein synthesis. Numerous mRNA-binding proteins (RBPs) have been described in multiple animal models that contribute to the control of mRNA translation and decay during oogenesis and spermatogenesis. An emerging view from studies performed in germ cells and somatic cells is that RBPs associate with their target mRNAs in RNA-protein (or ribonucleoprotein) complexes (mRNPs) that assemble in various cytoplasmic RNA granules that communicate with the translation machinery and control mRNA storage, triage, and degradation. In comparison with Xenopus, Caenorhabditis elegans, or Drosophila, the composition and role of cytoplasmic RNA-containing granules in mammalian germ cells are still poorly understood. However, regained interest for these structures has emerged with the recent discovery of their role in small RNA synthesis and transposon silencing through DNA methylation. In this review, we will briefly summarize our current knowledge on cytoplasmic RNA granules in murine germ cells and describe the role of some of the RBPs they contain in regulating mRNA metabolism and small RNA processing during gametogenesis.

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Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs

Cited by 58 publications

References 50 publications

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Translation of the synaptonemal complex component Sycp3 is enhanced in vivo by the germ cell specific regulator Dazl

RNA-binding proteins, RNA granules, and gametes: is unity strength?

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