DC discharge plasma modification of chitosan/gelatin/PLLA films: Surface properties, chemical structure and cell affinity

Демина, Т. С.; Zaytseva-Zotova, Daria; Yablokov, M. Yu.; Gilman, A. B.; Акопова, Т. А.; Марквичева, Елена; Зеленецкий, А. Н.

doi:10.1016/j.surfcoat.2012.07.059

Cited by 52 publications

(30 citation statements)

References 34 publications

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“…In a study aimed at the evaluation of plasma treatment effects on the surface properties of PLLA, film air plasma was used at a pressure of 10-20 Pa, and a discharge current of 50 mA for 60 s was applied. The CA of wettability measurements of the films showed that plasma modification led to an increase in hydrophilicity and SE [71]. The use of oxygen plasma treatment on fibril tip surfaces in polymer elastomeric polyurethane (PU) and polydimethylsiloxane (PDMS) fibrillar adhesives improved their wettability and adhesion capacity.…”

Section: Oxygen In Plasma Gas and The Surface Wettability Of Modifiedmentioning

confidence: 99%

Wettability and Other Surface Properties of Modified Polymers

Kasálková¹,

Slepička²,

Kolská³

et al. 2015

Wetting and Wettability

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Surface wettability is one of the crucial characteristics for determining of a material's use in specific application. Determination of wettability is based on the measurement of the material surface contact angle. Contact angle is the main parameter that characterizes the drop shape on the solid surface and is also one of the directly measurable properties of the phase interface. In this chapter, the wettability and its related properties of pristine and modified polymer foils will be described. The wettability depends on surface roughness and chemical composition. Changes of these parameters can adjust the values of contact angle and, therefore, wettability. In the case of pristine polymer materials, their wettability is unsuitable for a wide range of applications (such as tissue engineering, printing, and coating). Polymer surfaces can easily be modified by, e.g., plasma discharge, whereas the bulk properties remain unchanged. This modification leads to oxidation of the treated layer and creation of new chemical groups that mainly contain oxygen. Immediately after plasma treatment, the values of the contact angles of the modified polymer significantly decrease.In the case of a specific polymer, the strongly hydrophilic surface is created and leads to total spreading of the water drop. Wettability is strongly dependent on time from modification.Wettability plays a key role, e.g., in the development of biomaterials in tissue engineering and regenerative medicine. Biocompatibility tests of the cell adhesion, proliferation and viability are performed in an aqueous medium, and it is necessary to control the surface wettability. Various cell types have different requirements on surface properties, but while maintaining suitable parameters, the optimal value of © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.water contact angle for cell adhesion is in the interval of 50-70°. In the case of polymers, which have usually higher values of contact angles, the decrease in water contact angle and adjustment of the surface may be accomplished by introducing selected chemical groups (by plasma treatment), chemical compounds (by grafting, i.e., chemical bath deposition), or nanoparticles. In the case of grafting of polyethylene glycol (PEG) on the plasma activated high-density polyethylene was under "properly" selected conditions of modification (molecular weight of PEG, concentration of PEG in solution) prepared layers that positively influenced the cell adhesion. In addition, low concentration of gold nanoparticles increased the number of adhered cells without decreasing cell viability.Whether the surface of the polymeric substrate is attractive for the adhesion and proliferation of cells is determined not only by wettability but also by a range of other surface properties...

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Section: Oxygen In Plasma Gas and The Surface Wettability Of Modifiedmentioning

confidence: 99%

Wettability and Other Surface Properties of Modified Polymers

Kasálková¹,

Slepička²,

Kolská³

et al. 2015

Wetting and Wettability

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show abstract

“…Therefore, it can be assumed that the tested film indicates high wettability. Demina et al (2012) found out that DC discharge plasma modification of chitosan, gelatin and poly(L,L-lactide) films caused a reduction in the contact angle of wettability. They observed the decrease in contact angle of film from initial value of 76 to 22 % and 10 % after plasma treatment on cathode and anode, respectively.…”

Section: Contact Angle Measurementsmentioning

confidence: 99%

Antibacterial Activity and Physical Properties of Edible Chitosan Films Exposed to Low-pressure Plasma

Ulbin-Figlewicz

Zimoch-Korzycka

Jarmoluk

2014

Food Bioprocess Technol

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The challenge for food industry is developing gentle processes concept, which will prevent food spoilage and leave a food natural, minimally processed, fresh-like and safe. A new technique of food preservation could be usage of combined methods of green process, such as cold gas plasma with bioactive substances, and protective coatings. The aim of this study was to determine the antibacterial activity of chitosan films incorporated with lysozyme exposed to helium plasma treatment as well as evaluate their physical properties. The edible films have been prepared basing on low molecular weight chitosan by casting from lactic acid solution with water solution of lysozyme in three various concentrations (0, 0.5 and 1 %). Dried films were then modified by exposition on cold helium plasma treatment for 0, 5 and 10 min. Obtained films were tested against growth of Listeria monocytogenes, Yersinia enterocolitica and Pseudomonas fluorescens. In order to characterize chitosan-based films, their mechanical properties, theromogravimetric analysis (TGA), dynamic mechanical thermal analysis (DMTA), contact angle measurement, water vapour permeability (WVP) and scanning electron microscopy (SEM) were evaluated. The films with 1 % lysozyme incorporation enhanced the inhibition efficiency of chitosanbased films against gram-positive (L. monocytogenes) and gram-negative (P. fluorescens) bacteria, where reduction zones were 42.5 and 69.8 mm, respectively. Besides that, hydrolytic changes of chitosan chain caused by lysozyme activity were confirmed by TGA and DMTA. Contact angles and WVP of tested films were not significantly affected by helium plasma exposition, nor lysozyme dosage. Hydrophilic natures of chitosan-based films were confirmed by both tests.Microscopy image of cross-section structure was smooth and continuous due to lysozyme addition in film composition. Application of chitosan films incorporated with lysozyme and low-pressure plasma treatment could be used as innovative preservation method in a wide range of food products.

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“…Plasma modification of the film samples was carried out using a direct‐current (DC) discharge set‐up (Figure ) . The chitosan samples were placed in a vacuum reaction chamber (1) on one of two symmetrical parallel plate aluminum electrodes, namely cathode (2) or anode (3); 18 cm in diameter; 5 cm distance between the electrodes) and treated by DC discharge (800 V) induced from DC power supply (5).…”

Section: Methodsmentioning

confidence: 99%

DC Discharge Plasma Modification of Chitosan Films: An Effect of Chitosan Chemical Structure

Демина

Drozdova

Yablokov

et al. 2015

Plasma Processes & Polymers

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Surface plasma treatment of chitosan films is a widely used technique to control a variety of their properties, such as permeability, hemostatic activity and ability to support cell adhesion, proliferation and growth. Among other factors to be controlled, the chemical structure of initial chitosan is rarely taken into consideration. This work is aimed to highlight this factor by the example of low pressure direct-current discharge of various chitosan film samples. Contact angle measurements, X-ray photoelectron spectroscopy and scanning electron microscopy revealed differences in the film surface properties of the initial and plasma-treated films. The effect of the plasma treatment on cell viability of L929 mouse fibroblasts was studied by MTT-assay.

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DC discharge plasma modification of chitosan/gelatin/PLLA films: Surface properties, chemical structure and cell affinity

Cited by 52 publications

References 34 publications

Wettability and Other Surface Properties of Modified Polymers

Wettability and Other Surface Properties of Modified Polymers

Antibacterial Activity and Physical Properties of Edible Chitosan Films Exposed to Low-pressure Plasma

DC Discharge Plasma Modification of Chitosan Films: An Effect of Chitosan Chemical Structure

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