DC-LAMP stains pulmonary adenocarcinoma with bronchiolar Clara cell differentiation

Progenitor stem cell marker expression by pulmonary carcinomas

“…Other subtypes of adenocarcinomas show focal or isolated staining for these markers, similar to what has been shown previously. 16 By contrast, p63, which is a protein associated with epithelial stratification and squamous differentiation, 20,21 was expressed diffusely in 14% of adenocarcinomas, 27% of large cell carcinomas, and 0% of the small cell carcinomas studied. As expected, 100% of the squamous cell carcinomas showed diffuse and strong positivity for this marker.…”

“…Antibodies used were separated into different categories, such as embryonic stem cell marker, OCT-4 (Abcam, Cambridge, MA, USA; dilution 1:50); hematopoietic stem cell markers, such as CD34 (DAKO,USA; dilution 1:1000), KIT (DAKO; dilution 1:800), and CD133 (Miltenyi Biotec, USA; dilution 1:20); markers of asymmetric division, such as Musashi-1 (R&D, USA; dilution 1:800) and Musashi-2 (Protein Tech Group, USA; dilution 1:400); cell cycle differentiation markers, such as p21 (Calbiochem, USA; dilution 1:100); and markers for committed lineage, such as p63 (DAKO; dilution 1:800) for squamous/ basal cells, and CC10 and DC-Lamp markers for Clara cell. 16 Paraffin sections were de-paraffinized and submitted to antigen retrieval with citrate acid (pH 6) as previously described, 16 or with EDTA (pH 8) for the evaluation of p21. Staining was performed on an automated stainer (Ventana Medical System, Tucson, AZ, USA).…”

Long-Term Protective Effect of Mature DC-LAMP+ Dendritic Cell Accumulation in Sentinel Lymph Nodes Containing Micrometastatic Melanoma

“…DC-LAMP is most homologous to CD68, a lysosomal glycoprotein expressed by macrophages (12). Besides mature dendritic cells, DC-LAMP mRNA has been found in human and murine lungs and recently in pulmonary adenocarcinomas with Clara cell differentiation (13,14).…”

Bronchioloalveolar Carcinoma and Minimally Invasive Adenocarcinoma

“…note, there are no differences in prognosis or natural history of the disease from BAC that have Clara cell or type II pneumocyte differentiation; therefore, the distinction of the 2 cell types has no clinical relevance. 14 The paramount definition of BAC is that of a noninvasive tumor. Therefore, areas of fibrosis or scar associated with the tumor nodule and the presence of complex growth patterns such as acinar and papillary structures are exclusion criteria for the diagnosis of BAC.…”