DC-PMD, Dünnschicht-Chromatographie mit Gradienten-Elution im Vergleich zur Säulenflüssigkeits-Chromatographie

Bürger, K.

doi:10.1007/bf00528586

Cited by 66 publications

(8 citation statements)

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“…Burger (17) improved this technique but maintained the general principles of PMD. The Burger (17) method is called automated multiple development (AMD).…”

Section: E Automated Multiple Developmentmentioning

confidence: 95%

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Gradient Development in Thin-Layer Chromatography

Gotkiewicz¹

2003

Handbook of Thin-Layer Chromatography

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“…Burger (17) improved this technique but maintained the general principles of PMD. The Burger (17) method is called automated multiple development (AMD).…”

Section: E Automated Multiple Developmentmentioning

confidence: 95%

“…Burger (17) and Jaenchen (18,19) described a fully automatic machine for multiple development of a plate. An elution gradient is employed in accordance with the gradient program (see also Chap.…”

Section: A Devices For Achieving a Mobile-phase Gradientmentioning

confidence: 99%

Gradient Development in Thin-Layer Chromatography

Gotkiewicz¹

2003

Handbook of Thin-Layer Chromatography

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“…The AMD technique uses a solvent gradient and several development steps to separate compounds of widely different polarity. Most reported AMD applications have used gradients on normal phase plates [10][11][12][13][14][15][16][17][18][19]. This paper presents the qualitative and quantitative determination of rosmarinic acid, sinesetine and eupatorine from two extracts of Orthosiphon stamineus Benth.…”

Section: Figure 1 Structure Of Rosmarinic Acid (I) Sinesetin (Ii) Amentioning

confidence: 99%

Characterization of Orthosiphon Stamineus Benth extracts by reversed-phase thin layer Chromatographic methods

Oláh¹,

Hanganu²,

Bodoki³

et al. 2017

Studia UBB Chemia

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ABSTRACT. TLC is a powerful method used for separation of complex mixtures such as plant extracts. Employing different TLC techniques the separations can be improved. This paper presents a study of the Orthosiphon stamineus Benth extracts using isocratic reversed-phase thin layer chromatography (RP-TLC) and reversed-phase automated multiple development technique (RP-AMD). Methanol (SI) and a mixture of methanolwater-methyl acetate (SII) were used as extraction agents. Orthosiphon stamineus Benth. belongs to the Lamiaceae family. Its leaves contain rosmarinic acid, sinesetine and eupatorine as main compounds. After TLC separation the bioactive compounds from plant extracts were identified by comparison of the R f values and in situ UV-Vis spectra with those of the standards and quantified using the calibration method. The rosmarinic acid was better extracted in the solvent mixture methanol-water-methyl acetate (10:10:80, v/v), while the sinesetine and eupatorine, which are more lipophilic, were better extracted in methanol. The study revealed the AMD technique superiority in comparison with the isocratic one.

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“…A special device for an automated multiple developments (AMD) of a chromatogram was described by Perry et al (1973) and a programmable setup constructed by Burger (1984) and produced by Camag is known as AMD or AMD 2 system. A full separation process comprising 20-25 steps takes a long time.…”

Section: Automated Multiple Development Of Biomoleculesmentioning

confidence: 99%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

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DC-PMD, Dünnschicht-Chromatographie mit Gradienten-Elution im Vergleich zur Säulenflüssigkeits-Chromatographie

Cited by 66 publications

References 1 publication

Gradient Development in Thin-Layer Chromatography

Gradient Development in Thin-Layer Chromatography

Characterization of Orthosiphon Stamineus Benth extracts by reversed-phase thin layer Chromatographic methods

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

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