dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation

Haellman, Viktor; Martín, Pablo; Akmammedov, Arslan; Saxena, Pratik; Beerenwinkel, Niko; Paro, Renato; Teixeira, Augusto; Fussenegger, Martin

doi:10.1016/j.ymben.2022.06.003

Cited by 1 publication

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This system has been optimized for use in induced pluripotent stem cells, to control their differentiation toward desired cell lineages. [ 72,73 ]…”

Section: Transcription Regulation Relying On Natural Dna‐binding Domainsmentioning

confidence: 99%

“…This system has been optimized for use in induced pluripotent stem cells, to control their differentiation toward desired cell lineages. [72,73] More recent systems employ metabolites from bacterial central carbon metabolism. [6,7,9] In glucose-limiting conditions, Escherichia coli (E. coli) can utilize alternative carbon sources, including xylose and gluconate.…”

Section: Small-molecule-inducible Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Teixeira,

Fussenegger

2023

Advanced Science

Self Cite

View full text Add to dashboard Cite

Arming human cells with synthetic gene circuits enables to expand their capacity to execute superior sensing and response actions, offering tremendous potential for innovative cellular therapeutics. This can be achieved by assembling components from an ever‐expanding molecular toolkit, incorporating switches based on transcriptional, translational, or post‐translational control mechanisms. This review provides examples from the three classes of switches, and discusses their advantages and limitations to regulate the activity of therapeutic cells in vivo. Genetic switches designed to recognize internal disease‐associated signals often encode intricate actuation programs that orchestrate a reduction in the sensed signal, establishing a closed‐loop architecture. Conversely, switches engineered to detect external molecular or physical cues operate in an open‐loop fashion, switching on or off upon signal exposure. The integration of such synthetic gene circuits into the next generation of chimeric antigen receptor T‐cells is already enabling precise calibration of immune responses in terms of magnitude and timing, thereby improving the potency and safety of therapeutic cells. Furthermore, pre‐clinical engineered cells targeting other chronic diseases are gathering increasing attention, and this review discusses the path forward for achieving clinical success. With synthetic biology at the forefront, cellular therapeutics holds great promise for groundbreaking treatments.

show abstract

“…This system has been optimized for use in induced pluripotent stem cells, to control their differentiation toward desired cell lineages. [ 72,73 ]…”

Section: Transcription Regulation Relying On Natural Dna‐binding Domainsmentioning

confidence: 99%

Section: Small-molecule-inducible Systemsmentioning

confidence: 99%

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Teixeira,

Fussenegger

2023

Advanced Science

Self Cite

View full text Add to dashboard Cite

show abstract

dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation

Cited by 1 publication

References 60 publications

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Synthetic Gene Circuits for Regulation of Next‐Generation Cell‐Based Therapeutics

Contact Info

Product

Resources

About