In the rapid flooding process of a damaged ship, the air trapped in a damaged cabin will be compressed into an air cushion, which causes the air cushion effect (ACE). The ACE has a significant influence on the flooding process of an airtight cabin. In this paper, a three-cabin model is designed according to a parent ship, and a series of model experiments are carried out. First, the air-tightness ratio is defined by combining various air vents with different opening areas, and the flooding model experiments of different air-tightness ratios are carried out. The heeling and trimming angles of the model, the overpressure of the air cushion, and the water height inside the flooding cabin are recorded and analyzed, and the characteristics of ACE are summarized. Second, different forms of air vents with a same air-tightness ratio are designed, and the influence of the air vent distribution on the flooding process is analyzed. With the quasi-static method used for comparisons, the numerical results are compared with the experimental ones to check the effectiveness of the quasi-static method on the rapid flooding problems. The research on ACE can guide the design of ship subdivisions and provide a reference for the rescue of shipwreck accidents.