dCITI-Seq: droplet combinational indexed transposon insertion sequencing

Tu, Jing; Qiao, Yi; Xu, Zheyun; Lü, Na; Long, Naiyun; Zhang, Lu

doi:10.1007/s00216-022-03902-1

Cited by 3 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our investigation not only provides a thorough exposition of the comprehensive Tn5 assembly system but also attests to the high efficiency of Tn5 within the HetHydrogels. Looking ahead, we envisage the construction of Tn5 index libraries through the assembly of diverse insert sequences, [ 43 ] facilitating their application in single‐cell barcoding analyses. Furthermore, Tn5 variants harboring distinct insert sequences can be seamlessly integrated into reverse transcription reactions and multi‐step Tn5 reactions, [ 32 ] thereby enabling the realization of high‐dimensional single‐cell multi‐omics library construction processes.…”

Section: Discussionmentioning

confidence: 99%

Harnessing HetHydrogel: A Universal Platform to Dropletize Single‐Cell Multiomics

Zhou,

Li,

et al. 2024

Small Methods

View full text Add to dashboard Cite

A universal platform is developed for dropletizing single cell plate‐based multiomic assays, consisting of three main pillars: a miniaturized open Heterogeneous Hydrogel reactor (abbreviated HetHydrogel) for multi‐step biochemistry, its tunable permeability that allows Tn5 tagmentation, and single cell droplet barcoding. Through optimizing the HetHydrogel manufacturing procedure, the chemical composition, and cell permeation conditions, simultaneous high‐throughput mitochondrial DNA genotyping and chromatin profiling at the single‐cell level are demonstrated using a mixed‐species experiment. This platform offers a powerful way to investigate the genotype‐phenotype relationships of various mtDNA mutations in biological processes. The HetHydrogel platform is believed to have the potential to democratize droplet technologies, upgrading a whole range of plate‐based single cell assays to high throughput format.

show abstract

Section: Discussionmentioning

confidence: 99%

Harnessing HetHydrogel: A Universal Platform to Dropletize Single‐Cell Multiomics

Zhou,

Li,

et al. 2024

Small Methods

View full text Add to dashboard Cite

show abstract

Recent Innovations and Technical Advances in High‐Throughput Parallel Single‐Cell Whole‐Genome Sequencing Methods

Qiao,

Cheng,

Miao

et al. 2024

Small Methods

View full text Add to dashboard Cite

Single‐cell whole‐genome sequencing (scWGS) detects cell heterogeneity at the aspect of genomic variations, which are inheritable and play an important role in life processes such as aging and cancer progression. The recent explosive development of high‐throughput single‐cell sequencing methods has enabled high‐performance heterogeneity detection through a vast number of novel strategies. Despite the limitation on total cost, technical advances in high‐throughput single‐cell whole‐genome sequencing methods are made for higher genome coverage, parallel throughput, and level of integration. This review highlights the technical advancements in high‐throughput scWGS in the aspects of strategies design, data efficiency, parallel handling platforms, and their applications on human genome. The experimental innovations, remaining challenges, and perspectives are summarized and discussed.

show abstract

Droplet-based single-cell sequencing: Strategies and applications

Shang,

Wang,

et al. 2024

Biotechnology Advances

View full text Add to dashboard Cite

dCITI-Seq: droplet combinational indexed transposon insertion sequencing

Cited by 3 publications

References 29 publications

Harnessing HetHydrogel: A Universal Platform to Dropletize Single‐Cell Multiomics

Harnessing HetHydrogel: A Universal Platform to Dropletize Single‐Cell Multiomics

Recent Innovations and Technical Advances in High‐Throughput Parallel Single‐Cell Whole‐Genome Sequencing Methods

Droplet-based single-cell sequencing: Strategies and applications

Contact Info

Product

Resources

About